Carbon formvar grids

Lipids DOPC and DOPS, and PEG-PE, cholesterol and rhodamine-PE, the mini-extruder, extrusion membranes, and accessories were purchased from Avanti Polar Lipids. Endotoxin test cartridges (0.05–5.0 EU/mL) were purchased from Charles River. Accugene 10× TBE buffer, PCR tubes, and 96-well PCR plates (Axygen) were purchased from VWR. SYBR Safe and PicoGreen stains were purchased from Life Technologies Corporation. Agarose and cell lysis buffer were purchased from Lonza. Glycerol, Tris base, EDTA, Triton X-114, octyl β-d-glucopyranoside (OG), Tween20, magnesium chloride, magnesium sulfate, sodium chloride, and paraformaldehyde were purchased from Sigma-Aldrich. RPMI, PBS, FBS, and penicillin-streptomycin were purchased from Gibco. Carbon Formvar grids and uranyl formate were purchased from Electron Microscopy Sciences. The 96- and 384-well fluorescence assay plates were purchased from BD Biosciences. Amicon Ultra filtration devices, Optiprep media, and Seton ultracentrifugation tubes were purchased from Fisher Scientific.

Endotoxin test cartridges (0.05–5.0 EU/mL) were purchased from Charles River. Accugene 10× TBE buffer, PCR tubes, and 96-well PCR plates (Axygen) were purchased from VWR. SYBR Safe stain and CyQuant direct cell proliferation assay were purchased from Life Technologies Corporation. Agarose was purchased from Lonza. Glycerol, Tris base, EDTA, Triton X-114, Tween20, magnesium chloride, magnesium sulfate, and sodium chloride were purchased from Sigma-Aldrich. Actin was purchased from Cytoskeleton Inc. RPMI, DMEM, PBS, FBS, and penicillin-streptomycin were purchased from Gibco. Carbon Formvar grids and uranyl formate were purchased from Electron Microscopy Sciences. Amicon Ultra filtration devices and Seton ultracentrifugation tubes were purchased from Fisher Scientific. DNA gel extraction spin column was purchased from Bio-Rad.