For mES cells, similar western-blot experiments were carried out using antibodies against actin (Abcam), histone H3 (Abcam) and SNRP70 (Abcam). We confirmed NAI-N3 treatment didn’t affect fractionation. Protein was quantified using the Pierce BCA protein assay kit (Thermo Fischer Scientific). We loaded the protein from each compartment in proportion to the amount obtained (roughly 1:1:2 for chromatin:nucleoplasm:cytoplasm).
Snrp70
SNRP70 is a protein that plays a role in the spliceosome, a complex of small nuclear ribonucleoproteins (snRNPs) and other proteins that catalyze the removal of introns from precursor messenger RNA. SNRP70 is a component of the U1 snRNP, which is involved in the early stages of spliceosome assembly.
Lab products found in correlation
3 protocols using snrp70
Subcellular Fractionation and Molecular Analyses
Subcellular Fractionation and Molecular Analyses
For mES cells, similar western-blot experiments were carried out using antibodies against actin (Abcam), histone H3 (Abcam) and SNRP70 (Abcam). We confirmed NAI-N3 treatment didn’t affect fractionation. Protein was quantified using the Pierce BCA protein assay kit (Thermo Fischer Scientific). We loaded the protein from each compartment in proportion to the amount obtained (roughly 1:1:2 for chromatin:nucleoplasm:cytoplasm).
Antibody Panel for Cell Signaling Analysis
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