To obtain the FTIR spectra, 30 μL of the hydrogels were deposited onto disposable KBr infrared sample cards (Sigma-Aldrich, Rehovot, Israel), which were then allowed to dry under vacuum. The measurements were carried out in a nitrogen purged Nexus 470 FTIR spectrometer (Nicolet, Offenbach, Germany) equipped with a deuterated triglycine sulfate (DTGS) detector.
Kbr infrared sample cards
Manufactured by Merck Group
Sourced in Israel
KBr infrared sample cards are a common laboratory tool used in infrared spectroscopy. They provide a transparent medium for holding solid or liquid samples during infrared analysis. The cards are made of potassium bromide (KBr), a material that is transparent to infrared radiation, allowing the sample to be analyzed without interference from the card material.
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Lab products found in correlation
4 protocols using kbr infrared sample cards
1
FTIR Characterization of Hydrogels
2
FTIR Analysis of Peptide Structures
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FTIR spectroscopy was performed with 30 μl samples of peptide solutions, deposited onto disposable KBr infrared sample cards (Sigma-Aldrich, Rehovot, Israel), which were then allowed to dry under vacuum. Transmission infrared spectra were collected using a nitrogen-purged Nexus 470 FTIR spectrometer (Nicolet, Offenbach, Germany) equipped with a deuterated triglycine sulfate (DTGS) detector. Measurements were made by averaging 64 scans in 4 cm−1 resolution. The amide I region was deconvoluted by the second derivative method using the Peakfit software version 4.12 (Systat Software Inc., San Jose, CA, USA).
3
FTIR Characterization of F-Cu Nanosheets
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The F-Cu nanosheets were deposited on disposable KBr infrared sample cards (Sigma-Aldrich) and subsequently dried under vacuum. The spectra were collected from 4000 to 400 cm−1 at room temperature using a nitrogen-purged Nicolet Nexus 470 FTIR spectrometer (Nicolet) equipped with a deuterated triglycine sulfate detector. One hundred and twenty-eight scans were collected with a spectral resolution of 4 cm−1. The background was subtracted from a control KBr spectrum.
4
Peptide FTIR Spectroscopy Protocol
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A 30 µl aliquot of the peptide solution was deposited onto disposable KBr infrared sample cards (Sigma-Aldrich, Rehovot, Israel), which were then allowed to dry under open air and followed by vacuum. The samples were saturated twice with 30 µl of D2O and vacuum dried. FTIR spectra were collected using a nitrogen-purged Nicolet Nexus 470 FTIR spectrometer (Nicolet, Offenbach, Germany) equipped with a deuterated triglycine sulfate detector. Measurements were performed at 4 cm−1 resolution and obtained values were averaged over 32 scans. The absorbance maxima values were determined using an OMNIC analysis programme (Thermo Scientific Nicolet). The background was subtracted using a control spectrum.
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