All non-cancer cell lines used were human and were cultured at 37°C under 5% CO2 and 20% oxygen. THLE3 (normal liver) cells were a kind gift of the Kwan Yong Choi Lab (POSTECH, Korea). CCD841CoN (normal colon), CCD18Lu (normal lung), HS67 (normal thymus), HIEC6 (normal small intestine) were purchased from ATCC, and GM05565 and GM02037 (normal skin fibroblasts) were purchased from the Corriel Institute. THLE3 cells were cultured in BEBM Bronchial Epithelial Cell Growth Basal Medium (Lonza Cat#CC-3171) supplemented with the BEGM SingleQuots (Lonza Cat#CC-4175), 5ng/mL EGF, 70ng/mL Phosphoethanolamine, and 10% FBS. CCD841CoN, CCD18Lu, GM05565, and GM02037 were cultured in Eagle’s Minimum Essential Medium (ATCC Cat#30–2003) with 10% FBS (Sigma #F2442) and 100 units/ml Penicillin-Streptomycin. HS67 were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate) supplemented with 10% FBS, 100 units/ml Penicillin-Streptomycin, and 2mM L-Glutamine. HIEC6 were cultured in OptiMEM 1 Reduced Serum Medium (Gibco Cat#31985), 20 mM HEPES, 10 mM GlutaMAX (Gibco Cat#35050061), 10ng/ml EGF, 4% FBS, and 100 units/ml Penicillin-Streptomycin.
Hiec6
Manufactured by Thermo Fisher Scientific
Sourced in United States
The HIEC6 is a laboratory equipment designed for high-performance liquid chromatography (HPLC) analysis. It is a compact and versatile system that provides reliable and accurate separation and detection of a wide range of analytes.
Automatically generated - may contain errors
Lab products found in correlation
Glutamax, by Thermo Fisher Scientific (4 mentions)
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (3 mentions)
Ccd 841 con, by American Type Culture Collection (2 mentions)
Ccd 18lu, by American Type Culture Collection (2 mentions)
Gm05565, by American Type Culture Collection (2 mentions)
Bebm bronchial epithelial cell growth basal medium, by Lonza (2 mentions)
Gm02037, by American Type Culture Collection (2 mentions)
Hiec 6, by American Type Culture Collection (2 mentions)
Begm singlequots, by Lonza (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Caco 2, by Procell (1 mentions)
Sw480, by Procell (1 mentions)
Hct116, by Procell (1 mentions)
Hiec 6, by BioVector (1 mentions)
Ht 29, by BioVector (1 mentions)
Hepg2, by Thermo Fisher Scientific (1 mentions)
Atcc formulated eagle s minimum essential medium, by Thermo Fisher Scientific (1 mentions)
4 protocols using hiec6
1
Cell Line Culture Optimization and Maintenance
2
Cell Line Culture Optimization and Maintenance
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All non-cancer cell lines used were human and were cultured at 37°C under 5% CO2 and 20% oxygen. THLE3 (normal liver) cells were a kind gift of the Kwan Yong Choi Lab (POSTECH, Korea). CCD841CoN (normal colon), CCD18Lu (normal lung), HS67 (normal thymus), HIEC6 (normal small intestine) were purchased from ATCC, and GM05565 and GM02037 (normal skin fibroblasts) were purchased from the Corriel Institute. THLE3 cells were cultured in BEBM Bronchial Epithelial Cell Growth Basal Medium (Lonza Cat#CC-3171) supplemented with the BEGM SingleQuots (Lonza Cat#CC-4175), 5ng/mL EGF, 70ng/mL Phosphoethanolamine, and 10% FBS. CCD841CoN, CCD18Lu, GM05565, and GM02037 were cultured in Eagle’s Minimum Essential Medium (ATCC Cat#30–2003) with 10% FBS (Sigma #F2442) and 100 units/ml Penicillin-Streptomycin. HS67 were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose, pyruvate) supplemented with 10% FBS, 100 units/ml Penicillin-Streptomycin, and 2mM L-Glutamine. HIEC6 were cultured in OptiMEM 1 Reduced Serum Medium (Gibco Cat#31985), 20 mM HEPES, 10 mM GlutaMAX (Gibco Cat#35050061), 10ng/ml EGF, 4% FBS, and 100 units/ml Penicillin-Streptomycin.
3
Cultivation of Colorectal Cancer Cell Lines
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CRC cell lines (HCT116, Caco2, LoVo, SW480 and HT29) were obtained from Procell Life Science & Technology Co., Ltd. The normal colon epithelial cell line (HIEC-6) was obtained from BioVector Science Lab, Inc. The HT-29 cell line was authenticated by STR. HIEC-6 cells were cultured in DMEM (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.). CRC cell lines were cultured in DMEM (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS, 100 U/ml penicillin and 100 µg/ml streptomycin (both from Gibco; Thermo Fisher Scientific, Inc.) All cell lines were cultured in a humidified incubator at 37˚C in a 5% CO2 atmosphere.
4
Culturing HIEC-6 and HepG2 Cells
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HIEC-6 (ATCC® CRL-3266™) and HepG2 (ATCC® HB-8065™) cells were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA). HIEC-6 cells were cultured using OptiMEM 1 Reduced Serum Medium (Thermo Fisher Scientific, Gibco No. 31985, Waltham, MA, USA) with 20 mM HEPES, 10 mM GlutaMAX (Thermo Fisher Scientific, Gibco No. 35050, Waltham, MA, USA), 10 ng/mL Epidermal Growth Factor, and 4% fetal bovine serum (FBS). HepG2 cells were grown using ATCC-formulated Eagle’s Minimum Essential Medium (Thermo Fisher Scientific, Gibco No. 30-2003, Waltham, MA, USA), with 10% FBS. Cells were maintained at 37 °C with 90% relative humidity and 5% CO2 in culture medium.
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