Sodium pyruvate

Manufactured by Cytiva

Sourced in United States

Sodium pyruvate is a salt compound that serves as a key intermediate in cellular metabolism. It is a source of pyruvate, an important molecule in the glycolytic pathway and the Krebs cycle. Sodium pyruvate is commonly used in cell culture media to support cell growth and maintain cellular energy production.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (4 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Hepes, by Cytiva (3 mentions) L glutamine, by Cytiva (3 mentions) Rpmi 1640, by Cytiva (3 mentions) Hepes, by Thermo Fisher Scientific (3 mentions) Dexamethasone (dex), by MP Biomedicals (2 mentions) Dmem hg, by Merck Group (2 mentions) Non essential amino acids (neaa), by Lonza (2 mentions) Its premix, by Corning (2 mentions) 2 7 dichlorofluorescein dcfh, by Merck Group (2 mentions) Lamivudine 3tc, by Merck Group (2 mentions) Non essential amino acids (neaa), by Cytiva (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (2 mentions) Heat inactivated fetal bovine serum, by Merck Group (1 mentions) Polycarbonate transwells, by Corning (1 mentions) Dynabeads m 450, by Thermo Fisher Scientific (1 mentions) Tgf β1, by Thermo Fisher Scientific (1 mentions) Agarose, by Thomas Scientific (1 mentions)

17 protocols using sodium pyruvate

Murine Cell Line Culture Protocols

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The murine P3X63Ag8 (CRL-1580; termed P3X herein) plasma cell line and A20 (TIB-208) B cell line were purchased from the American Type Tissue Collection. All murine cells were grown in RPMI 1640 medium (Mediatech, Inc.) supplemented with 10% heat-inactivated fetal bovine serum (SIGMA, Inc.), 10 mM HEPES (HyClone Laboratories), 1 mM sodium pyruvate (HyClone Laboratories), 1X non-essential amino acids (HyClone Laboratories), and 0.05 mM 2-ME (Sigma-Aldrich).

Majumder P., Scharer C.D., Choi N.M, & Boss J.M. (2014). B cell differentiation is associated with reprogramming the CTCF-dependent chromatin architecture of the murine MHC-II locus. Journal of immunology (Baltimore, Md. : 1950), 192(8), 3925-3935.

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Magnetic Particle-Enhanced Chondrogenesis

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Serum proteins were non-specifically adsorbed onto the surface of magnetic particles (“MP”; DynaBeads M-450, ThermoFisher Scientific) by incubating 100 µl particle solution (4×10⁸ MPs/ml) with 900 µl FBS at room temperature overnight on a rotisserie rotator (Labquake, Barnstead Thermolyne) to facilitate particle incorporation into the cell sheet. Cell culture inserts (3.0 µm pore, 6.5 mm diameter polycarbonate transwells, Corning) were incubated with 450 µl DMEM-LG with 10% FBS in the wells of a 24-well plate for 2 hours. Next, 0.6×10⁶ hMSCs were mixed with 0.6×10⁶ MPs (“hMSC + MP”) and seeded onto the insert in 100 µl chondrogenic media consisting of Dulbecco's Modified Eagle's Medium - high glucose (DMEM-HG; Sigma-Aldrich), 1% ITS+ Premix (Corning), 10⁻⁷ M dexamethasone (MP Biomedicals), 1 mM sodium pyruvate (HyClone Laboratories), 100 mM non-essential amino acids (Lonza Group), 37.5 mg/ml ascorbic acid-2-phosphate (Wako Chemicals USA) and 10 ng/ml transforming growth factor beta 1 (TGF-β1, PeproTech). Lastly, an additional 450 µl of chondrogenic media was added to the plate well. Control sheets without MPs (“hMSC”) were prepared in the same manner. hMSC ± MP were cultured in 1 ml chondrogenic medium replaced every 2 days for 3 weeks.

Dikina A.D., Lai B.P., Cao M., Zborowski M, & Alsberg E. (2017). Magnetic field application or mechanical stimulation via magnetic microparticles does not enhance chondrogenesis in mesenchymal stem cell sheets. Biomaterials science, 5(7), 1241-1245.

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Agarose Molds for 3D Cell Culture

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agarose molds for cell culture were prepared as previously described [34 (link)]. Briefly, a polycarbonate sheet (Small Parts Inc., Miramar, FL) was machined to contain annular wells with concentric 2 mm diameter posts surrounded by a 3.75 mm wide trough. A polydimethylsiloxane (PDMS; Sylgard 184, Dow Corning, Midland, MI) negative mold of the polycarbonate template was cured and steam autoclaved for sterilization. Two percent w/v agarose (Denville Scientific Inc., Metuchen, NJ) in DMEM-LG was autoclaved and used to fill the PDMS mold. After cooling, the ring-shaped culture wells were removed from the PDMS mold, moved into 6-well plates (BD, Franklin Lakes, NJ) and incubated overnight in basal pellet medium (BPM) comprised of Dulbecco’s Modified Eagle’s Medium - high glucose (DMEM-HG; Sigma-Aldrich), 1% ITS+ Premix (Corning Inc, Corning, NY), 10⁻⁷ M dexamethasone (MP Biomedicals, Solon, OH), 1 mM sodium pyruvate (HyClone Laboratories), 100 µM non-essential amino acids (Lonza Group, Basel, Switzerland), 37.5 µg/ml ascorbic acid-2-phosphate (Wako Chemicals USA Inc.) and 100 u/ml penicillin-streptomycin (Corning Inc.).

Dikina A.D., Strobel H.A., Lai B.P., Rolle M.W, & Alsberg E. (2015). Engineered cartilaginous tubes for tracheal tissue replacement via self-assembly and fusion of human mesenchymal stem cell constructs. Biomaterials, 52, 452-462.

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Hepatoma Cell Line Protocol

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Human hepatoma cells (HepG2) and HBV cell line, HepG2.2.2.15 (derivative of HepG2) (kind gift of Dr. S. Jameel, International Center for Genetic Engineering & Biotechnology, New Delhi, India), were grown in RPMI-1640 medium (Invitrogen, USA), supplemented with 10% heat-inactivated bovine serum (Gibco, USA), 1x penicillin-streptomycin (Invitrogen, USA), and 1x sodium pyruvate (HyClone Laboratories, USA) at 37°C in a humidified chamber with 5% CO₂ supply. 2,7-Dichlorofluorescein (DCFH; Sigma, USA) was used as cytotoxin on cultured HepG2 cells. The approved nucleoside analog-based anti-HBV drug, Lamivudine (3TC; Sigma, USA), was used as standard.

Arbab A.H., Parvez M.K., Al-Dosari M.S., Al-Rehaily A.J., Al-Sohaibani M., Zaroug E.E., AlSaid M.S, & Rafatullah S. (2015). Hepatoprotective and Antiviral Efficacy of Acacia mellifera Leaves Fractions against Hepatitis B Virus. BioMed Research International, 2015, 929131.

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Culturing HepG2.2.15 Cells for HBV Studies

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HBV-reporter cells, HepG2.2.15 (human hepatoblastoma line, HepG2-derived) were kindly obtained from Dr. S. Jameel (International Center for Genetic Engineering & Biotechnology, New Delhi, India). HepG2.2.15 cell were maintained in RPMI-1640 medium (Invitrogen, USA), supplemented with 10% heat-inactivated bovine calf serum (Gibco, USA), 1x penicillin-streptomycin (Invitrogen, USA), and 1x sodium pyruvate (HyClone Laboratories, USA) at 37 ⁰C in a humidified chamber with 5% CO₂ supply.

Parvez M.K., Tabish Rehman M., Alam P., Al-Dosari M.S., Alqasoumi S.I, & Alajmi M.F. (2018). Plant-derived antiviral drugs as novel hepatitis B virus inhibitors: Cell culture and molecular docking study. Saudi Pharmaceutical Journal : SPJ, 27(3), 389-400.

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Cultivating Cancer Cell Lines for Assays

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The hepatocellular carcinoma cell lines Hep G2 and Hep 3B, the colorectal carcinoma cell line HT-29, and the pancreatic adenocarcinoma cell line Panc-1 were obtained from the American Type Culture Collection (ATCC^®, Manassas, VA, USA). Catalog number and passage number are provided in the supplementary material. Hep G2 and Hep 3B cell lines were cultured in Eagle's Minimal Essential Medium (EMEM) +2 mM L-Glutamine (Lonza, Walkersville, MD, USA), HT-29 in Mc Coy's 5A + 1.5 mM L-Glutamine, +2.2 g/L Sodium Bicarbonate and Panc-1 in Dulbecco's Modified Essential Medium (DMEM) + 4.5 g/L D-Glucose, +2 mM L-Glutamine, +110 mg/L Sodium Pyruvate (HyClone Laboratories, Logan, UT, USA). All medias were supplemented with 10% fetal calf serum (FCS) (HyClone Laboratories, Logan, UT, USA) and Penicillin/Streptomycin; 100 μg/ml each (Gibco, Grand Island, NY, USA). Cells were cultured in a humidified atmosphere of 5% CO₂ at 37 °C. For testing, cells were seeded in black walled, clear bottom 96 well plates (1 × 10⁴ cells per well; cytotoxicity, caspase 3/7 activity, reactive oxygen species & TUNEL assay) or in 24 well plates for radioactive experiments (7.5 × 10⁴ cells per well) one day prior to treatment and testing.

Ludwig J.M., Gai Y., Sun L., Xiang G., Zeng D, & Kim H.S. (2016). SW43‐DOX ± loading onto drug‐eluting bead, a potential new targeted drug delivery platform for systemic and locoregional cancer treatment – An in vitro evaluation. Molecular Oncology, 10(7), 1133-1145.

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Assessing Anti-HBV Activity of Compounds

Cited 1 time

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The HBV reporter
hepatoma cell line HepG2.2.15 cells (kind gift of
Dr. S. Jameel, International Center for Genetic Engineering &
Biotechnology, New Delhi, India) were grown and maintained in RPMI-1640
medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco,
USA), 1× penicillin–streptomycin mix, and 1× sodium
pyruvate (HyClone Laboratories, USA) at 37 °C in a humid chamber
with 5% CO₂ supply. The approved nucleoside analogue-based
anti-HBV drug lamivudine (Sigma-Aldrich, Germany) and the anti-HBV-active
natural compound quercetin (Sigma-Aldrich, Germany) were used as standard
or positive controls.^{32 (link),60 (link)} Stocks of E. schimperi methanol extract, the isolated compounds, and standards were prepared
in RPMI after dissolving in DMSO (<1%, final) as mentioned elsewhere.^{13 (link)} DMSO served as the vehicle or negative control.

Parvez M.K., Ahmed S., Al-Dosari M.S., Abdelwahid M.A., Arbab A.H., Al-Rehaily A.J, & Al-Oqail M.M. (2021). Novel Anti-Hepatitis B Virus Activity of Euphorbia schimperi and Its Quercetin and Kaempferol Derivatives. ACS Omega, 6(43), 29100-29110.

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HepG2 Hepatocyte Cell Culture Protocol

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Human liver carcinoma cell line HepG2 and its derivative HBV reporter line HepG2.2.15 (kind gift of Dr. S. Jameel, International Center for Genetic Engineering & Biotechnology, New Delhi, India). Cells were grown and maintained in RPMI-1640 medium (Gibco, USA), supplemented with 10% heat-inactivated bovine serum (Gibco, USA), 1xpenicillin-streptomycin mix, and 1x sodium pyruvate (HyClone Laboratories, USA) at 37 ⁰C in a humified chamber with 5% CO₂ supply. 2,7-Dichlorofluorescein (DCFH; Sigma, USA) was used as an inducer of in vitro hepatotoxicity. The approved nucleoside analog-based anti-HBV drug, lamivudine (3TC; Sigma, USA), was used as standard.

Parvez M.K., Al-Dosari M.S., Arbab A.H, & Niyazi S. (2019). The in vitro and in vivo anti-hepatotoxic, anti-hepatitis B virus and hepatic CYP450 modulating potential of Cyperus rotundus. Saudi Pharmaceutical Journal : SPJ, 27(4), 558-564.

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Transcriptomic Analysis of T Cell Subsets

Cited 2 times

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CD3⁺ T cells were enriched from frozen PBMCs using negative magnetic bead selection (Miltenyi Biotec) and cultured in media (RPMI-1640, penicillin/streptomycin/glutamine, sodium-pyruvate (all Cytiva HyClone) and 1% human serum (Access Cell Culture)) with or without IL-6 (20 ng/mL, BD Biosciences) for 4 h at 37°C. Cells were then stained for viability in PBS for 10 min with the live/dead blue fixable dye (ThermoFisher) and stained for CD3, CD4, CD8, CD45RA, and CD45RO see Supplementary Table 2 for 20 min in FACS buffer. Cells were sorted as CD4⁺ naïve (live CD3⁺CD4⁺CD45RA⁺), CD4⁺ memory (live CD3⁺CD4⁺CD45RO⁺) and CD8⁺ naïve (live CD3⁺CD8⁺CD45RA⁺) subsets and 1000 cells of each population were flow sorted and captured in PCR tubes containing 1× reaction buffer (10X lysis buffer, RNase inhibitor, nuclease-free H₂O, Cell Signaling Technology) and analyzed by RNA-seq as previously described (30 (link)). Low-quality libraries (median coefficient of variation (CV) of coverage > 0.9, total reads <2.5 million) were excluded. Counts were then normalized and log₂ transformed, followed by batch correction using the limma R package (31 (link)). Gene set enrichment analysis was performed using the Molecular Signatures Database v7.5.1 (MSigDB) Gene Ontology Biological Processes (GO : BP) collection (32 (link), 33 (link)). R software was used to display enriched pathways when indicated.

Lambert K., Diggins K.E., Jones B.E., Hundhausen C., Maerz M.D., Hocking A.M., Sanda S., Greenbaum C.J., Linsley P.S., Cerosaletti K, & Buckner J.H. (2022). IL-6-Driven pSTAT1 Response Is Linked to T Cell Features Implicated in Early Immune Dysregulation. Frontiers in Immunology, 13, 935394.

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Culturing Myeloma Cell Lines

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Raji (ATCC, CCL-86, 70053718), MM.1s (ATCC, CRL-2974, 70042525), and RS4;11 (ATCC, CRL-1873, 70036117) were thawed from liquid nitrogen and cultured in RPMI-1640 (Cytiva, SH30027.01) with 10% FBS, HEPES (Gibco, 15630-080), Sodium Pyruvate (Cytiva, SH30239.01), and Penicillin/Streptomycin (P/S, Gibco, 15140-122). Cells were kept at 37°C and 5% CO 2 . Cells were maintained by the addition of media every one to three days.

Cracchiolo M.J., Davis L., Matiatos A.P., Davini D.W., Husnain M., Simpson R.J., Voudouris V, & Katsanis E. (2024). Comparable efficacy of oral bendamustine versus intravenous administration in treating hematologic malignancies. Cancer chemotherapy and pharmacology.

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