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Isotope labeled l arginine hcl d7 arginine

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Isotope labeled L-arginine:HCl (D7-arginine) is a stable isotope-labeled amino acid. It is composed of the amino acid L-arginine with seven deuterium atoms replacing hydrogen atoms.

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Lab products found in correlation

L arginine, by Merck Group (5 mentions) L citrulline, by Merck Group (5 mentions) Leucine enkephalin, by Waters Corporation (5 mentions) Sodium tetraborate, by Merck Group (5 mentions) Methanol, by Merck Group (4 mentions) Formic acid, by Merck Group (4 mentions) Asymmetric dimethylarginine 2 3 3 4 4 5 5 d7 adma, by Cambridge Isotopes (3 mentions) Acetonitrile, by Merck Group (3 mentions) L ornithine monohydrochloride, by Merck Group (3 mentions) Asymmetric dimethylarginine, by Cambridge Isotopes (2 mentions) Hydrochloride salts of unlabeled dimethylamine, by Merck Group (2 mentions) Benzoyl chloride bcl, by Merck Group (2 mentions) Labeled l ornithine hydrochloride, by Merck Group (2 mentions) Acetonitrile acn, by Merck Group (1 mentions) Benzoyl chloride, by Merck Group (1 mentions) Lc ms grade acetonitrile, by Merck Group (1 mentions)

5 protocols using isotope labeled l arginine hcl d7 arginine

1

Quantification of Methylated Arginine Analogs

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Benzoyl chloride (BCl), hydrochloride salts of unlabeled dimethylamine (DMA), hexadeutero-dimethylamine (D6-DMA, 99%), L-arginine, SDMA, ADMA, L-citrulline, and sodium tetraborate were procured from Sigma-Aldrich (Poznan, Poland). Isotope labeled L-arginine:HCl (D7-arginine, 98%) and asymmetric dimethylarginine (2,3,3,4,4,5,5-D7-ADMA, 98%) were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). Methanol, acetonitrile, water, and formic acid were acquired from Merck Millipore (Warsaw, Poland), and leucine–enkephalin was from Waters (Milford, MA, USA).
Fleszar M.G., Wiśniewski J., Zboch M., Diakowska D., Gamian A, & Krzystek-Korpacka M. (2019). Targeted metabolomic analysis of nitric oxide/L-arginine pathway metabolites in dementia: association with pathology, severity, and structural brain changes. Scientific Reports, 9, 13764.
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2

Arginine Metabolite Analysis Protocol

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Benzoyl chloride (BCl), hydrochloride salts of unlabeled dimethylamine (D0-DMA), hexadeutero-dimethylamine (D6-DMA, declared as 99 at.% 2H), l-arginine, SDMA, ADMA, l-citrulline, and sodium tetraborate were procured from Sigma-Aldrich (Poznan, Poland). Isotope-labeled l-arginine:HCl (D7-arginine, 98%) and asymmetric dimethylarginine (2,3,3,4,4,5,5-D7-ADMA, 98%) were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). Methanol, acetonitrile, water, and formic acid were acquired from Merck Millipore (Warsaw, Poland), and leucine–enkephalin was obtained from Waters (Milford, MA, USA).
Fleszar M.G., Wiśniewski J., Krzystek-Korpacka M., Misiak B., Frydecka D., Piechowicz J., Lorenc-Kukuła K, & Gamian A. (2018). Quantitative Analysis of l-Arginine, Dimethylated Arginine Derivatives, l-Citrulline, and Dimethylamine in Human Serum Using Liquid Chromatography–Mass Spectrometric Method. Chromatographia, 81(6), 911-921.
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3

Arginine/Nitric Oxide Pathway Metabolites

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The Arg/NO pathway metabolites were of analytical standard: hydrochloride salts of unlabeled dimethylamine (DMA), hexadeutero- dimethylamine (D6-DMA, declared as 99 atom% 2H), L-arginine, SDMA, ADMA, L-citrulline, L-ornithine monohydrochloride, labeled L-ornithine hydrochloride (3,3,4,4,5,5-D6-ornithine), benzoyl chloride (BCl) and sodium tetraborate were procured from Sigma-Aldrich (Poznan, Poland). Isotope labeled L-arginine: HCl (D7-arginine, 98%) and asymmetric dimethylarginine (2,3,3,4,4,5,5-D7-ADMA, 98%) were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). Methanol, acetonitrile (ACN), water, and formic acid (FA) were acquired from Merck Millipore (Warsaw, Poland), and leucine–enkephalin was obtained from Waters (Warsaw, Poland) [34 (link),35 ].
Leśnik P., Łysenko L., Fleszar M.G., Fortuna P., Woźnica-Niesobska E., Mierzchała-Pasierb M, & Janc J. (2022). Measurement of Serum Levels of 5 Amino Acids and Dimethylamine Using Liquid Chromatography-Tandem Mass Spectrometry in Patients without Septic Associated Acute Kidney Injury and with Septic Associated Acute Kidney Injury Requiring Continuous Renal Replacement Therapy. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 28, e937784-1-e937784-10.
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4

Targeted Metabolomic Analysis of Methylarginines

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Benzoyl chloride (BCl), hydrochloride salts of unlabeled dimethylamine (D0-DMA), hexadeutero-dimethylamine (D6-DMA, declared as 99 atom % 2H), L-arginine, SDMA, ADMA, L-citrulline, L-Ornithine monohydrochloride, labeled L-Ornithine hydrochloride (3,3,4,4,5,5-D6-ornithine), and sodium tetraborate were procured from Sigma-Aldrich (Poznan, Poland). Isotope labeled L-arginine:HCl (D7-arginine, 98%) and asymmetric dimethylarginine (2,3,3,4,4,5,5-D7-ADMA, 98%) were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). Methanol, acetonitrile, water, and formic acid were acquired from Merck Millipore (Warsaw, Poland), and leucine–enkephalin was obtained from Waters (Milford, MA, USA).
Bednarz-Misa I., Fortuna P., Fleszar M.G., Lewandowski Ł., Diakowska D., Rosińczuk J, & Krzystek-Korpacka M. (2020). Esophageal Squamous Cell Carcinoma Is Accompanied by Local and Systemic Changes in L-arginine/NO Pathway. International Journal of Molecular Sciences, 21(17), 6282.
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5

Quantification of Methylated Arginine Derivatives

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LC/MS grade acetonitrile, methanol, and water were obtained from Merck Millipore (Warsaw, Poland). Analytical standards: hydrochloride salts of unlabeled dimethylamine (D0-DMA), hexadeutero- dimethylamine (D6-DMA, declared as 99 atom % 2H), L-arginine, SDMA, ADMA, L-citrulline, L-ornithine monohydrochloride, labeled L-ornithine hydrochloride (3,3,4,4,5,5-D6-ornithine), benzoyl chloride (BCl) and sodium tetraborate were procured from Sigma-Aldrich (Poznan, Poland). Isotope labeled L-arginine:HCl (D7-arginine, 98%) and asymmetric dimethylarginine (2,3,3,4,4,5,5-D7-ADMA, 98%) were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). Leucine–enkephalin was purchased from Waters (Milford, MA, USA).
Bednarz-Misa I., Fleszar M.G., Fortuna P., Lewandowski Ł., Mierzchała-Pasierb M., Diakowska D, & Krzystek-Korpacka M. (2021). Altered L-Arginine Metabolic Pathways in Gastric Cancer: Potential Therapeutic Targets and Biomarkers. Biomolecules, 11(8), 1086.
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