Silicon phthalocyanine dichloride (SiPCCl2), 3‐aminopropyltrimethoxysilane (APTES), Hoechst 33342, tetraethylorthosilicate (TEOS), and 2, 7‐dichlorodihydroflfluorescein diacetate (DCFH‐DA) were obtained from Sigma‐Aldrich. Hexadecyl trimethylammonium chloride (CTAC), triethanolamine (TEA), and FeCl3 were obtained from Sinopharm Chemical Reagent Co. Ltd. Dylight550‐NHS was purchased from Thermo Fisher Scientific (Waltham, USA). ICG‐NHS was obtained from Xi'an Rixi Biological Technology Co. Ltd (Xi'an, China). Calcein‐AM/propidium iodide (PI), and 1, 3‐diphenylisobenzofuran (DPBF) were obtained from KeyGen BioTech (Shanghai, China). Annexin V‐FITC/PI and CCK8 were obtained from Dojindo Laboratories. Annexin V‐APC/PI was obtained from Jiangsu KeyGEN BioTECH Corp., Ltd (Jiangsu, China) Neutrophil (mouse) isolation Kit was purchased from Cayman Chemical (Michigan, USA). Anti‐CD11c‐APC, anti‐CD137‐APC, anti‐CD11b‐APC, anti‐CD86‐PE‐Cy7, antiCD3‐APC, anti‐CD80‐PE, anti‐CD8‐PE, anti‐Ly‐6G/Ly‐6C‐PE, CD54, CD95, anti‐CD8 antibody, and anti‐CD69‐FITC was purchased from BioLegend, Inc (San Diego, CA, USA). ELISA kits from Neobioscience Technology (Shenzhen, China) were obtained to detect the IFN‐γ, TNF‐α, IL‐12, and IL‐2. H22 mouse liver cancer cell‐specific neoantigen (sequence: HTDAHAQAFAALFDSMH) was obtained from GenScript USA Inc.
Anti cd137 apc
Manufactured by BioLegend
Sourced in United States
Anti-CD137-APC is a fluorescently labeled antibody that binds to the CD137 receptor, also known as 4-1BB. CD137 is a costimulatory molecule expressed on the surface of activated T cells and natural killer cells. The APC (Allophycocyanin) fluorescent label allows for the detection and analysis of CD137-expressing cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd137 rabbit mab, by Cell Signaling Technology (5 mentions)
Sybr primescript rt pcr kit, by Takara Bio (5 mentions)
Trizol, by Thermo Fisher Scientific (5 mentions)
Rnalater, by Thermo Fisher Scientific (5 mentions)
Mojosort magnet, by BioLegend (5 mentions)
Mojosort human cd8 nanobeads, by BioLegend (5 mentions)
Agonistic anti cd137 mab, by BPS Biosciences (4 mentions)
Anti cytokeratin mouse mab, by Abcam (4 mentions)
Mojosort human cd8 cell isolation kit, by BioLegend (4 mentions)
Anti cd3 fitc, by BioLegend (3 mentions)
Anti cd45 percp, by BioLegend (3 mentions)
Anti cd8 apc, by BioLegend (3 mentions)
Anti cd8 mouse antibody 66868 1 ig, by Proteintech (2 mentions)
Anti cd8 mouse antibody 66868 1 ig for ihc, by Proteintech (2 mentions)
Human cd3 mab okt3, by BioLegend (2 mentions)
Anti cd8 antibody, by BioLegend (1 mentions)
Anti cd11b apc, by BioLegend (1 mentions)
Anti cd11c apc, by BioLegend (1 mentions)
Anti cd8 pe, by BioLegend (1 mentions)
Dylight 550 nhs, by Thermo Fisher Scientific (1 mentions)
8 protocols using anti cd137 apc
1
Multimodal Nanoplatform for Cancer Theranostics
2
Activated T cell Immunophenotyping in Mice
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Adequate single cells were isolated from the spleen, peripheral blood, colon adjacent tissues, and adenoma tissues of mice. These cells were incubated with the appropriate surface antibodies: anti-CD45-PE/Cy7 (Biolegend, Cat# 103113), anti-CD3-PE/Cy7 (Biolegend, Cat# 100219), anti-CD4-FITC (Invitrogen, 11-0041-85), anti-CD8a-APC (Invitrogen, 47-0081-82), anti-CD11b-FITC (Biolegend, Cat# 101206), anti-Gr-1-PE (Biolegend, Cat# 108408), anti-CD107a-FITC (Biolegend, Cat# 121605), and anti-CD137-APC (Biolegend, Cat# 106109). For intracellular staining, cells were treated with the Fixation/Permeabilization solution (Invitrogen, 00-5223-56) overnight at 4 °C before incubating with intracellular antibodies: anti-IFNγ-PE (Biolegend, Cat# 163503). CD4+ T cells were defined as CD45+CD4+CD8a−; CD8+ T cells were defined as CD45+CD4−CD8a+; activated CD4+ T cells were defined as CD3+CD4+CD137+IFNγ+; and activated CD8+ T cells were defined as CD3+CD8a+CD107a+IFNγ+ [23 (link),24 (link)]. Data acquisition was performed on an Accuri C6 Plus and analyzed using FlowJo (RRID:SCR_008520).
3
Multiparameter Flow Cytometry Analysis
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Flow cytometry analyses were performed on an LSR Fortessa flow cytometer (BD Biosciences). Automatic compensation was performed using CompBeads (BD Biosciences). Fluorescence minus one controls were performed to define gates of positivity. Following antibodies and reagents were used: biotinylated anti-CD11b, anti-CD33 PE-Cy7, anti-HLA-DR PE-Cy7, anti-CD15 FITC, anti-CD8 PE, anti-CD4 APC-Hy, anti-CD127 FITC, anti-CD16 FITC, anti-CD45 RO PE, andi-CD45 RA FITC, anti-CD95 PD-CF594, anti-PD-1 APC, anti CD62L APC (BD Biosciences); anti-CD14 APC-Cy7, anti-CD3 BV 605, anti-CD3 PE/Dazzle 594, anti-CD4 PE-Cy7, anti-CD4 PerCPCy5.5, anti-CD56 PE-Cy7, andi-CD28 FITC, anti-CD27 APC, anti-ICOS APC-Cy7, anti-CD137 PE, anti-CD137 APC, Zombie Yellow Fixable Viability Kit (BioLegend); eFluor 450 labeled streptavidin, anti-CD8 APC-H7, anti-Foxp3 APC, anti-CCR7 APC, anti-TIM3 eFluor 450, anti-TIGIT PE, anti-LAG3 PerCPeFluor710 (eBioscience), anti-CD25 PE (Myltenyi Biotec) and anti-OX40 APC (R&D Systems).
4
Evaluation of Anti-CD137 Immunotherapy
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RNAlater® was purchased from Ambion, USA. TRIzol was purchased from Invitrogen, USA. DEPC was purchased from Bio Basic Inc, Canada. The SYBR® PrimeScript® RT-PCR Kit was purchased from TaKaRa, Japan for two-step RT-PCR. PCR primers were designed by TaKaRa, Japan and synthesized by Yingjun Biotechnology Co., Ltd, China. An anti-CD137 rabbit mAb (#34549) used for IHC and IF and was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST, USA. An agonistic anti-CD137 mAb (#79097) was purchased from BPS Bioscience, USA. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST, USA. Anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from the Proteintech group, China. MojoSort™ Magnet, MojoSort™ Human CD8 Nanobeads and MojoSort™ Human CD8 Cell Isolation Kit were purchased from BioLegend, USA. An NF-κB p65 rabbit mAb (#8242) for flow cytometry and IF was purchased from CST, USA. An anti-cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam, England. A purified anti-human CD3 mAb (OKT3, #317326) for cell incubation and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for flow cytometry were purchased from BioLegend, USA.
5
Immune Checkpoint Receptor Analysis Protocol
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RNAlater® was purchased from Ambion. TRIzol was purchased from Invitrogen. DEPC was purchased from Bio Basic Inc. The SYBR®PrimeScript® RT-PCR Kit was purchased from TaKaRa for two-step RT-PCR. PCR primers were designed by TaKaRa and synthesized by Yingjun Biotechnology Co., Ltd. An anti-CD137 rabbit mAb (#34549) used for IHC and IF was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST. An agnostic anti-CD137 mAb (#79097) was purchased from BPS Bioscience. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST. An anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from Proteintech group. MojoSort TM Magnet, MojoSort TM Human CD8 Nanobeads and MojoSort TM Human CD8 Cell Isolation Kit were purchased from BioLegend. A NF-κB p65 rabbit mAb (#8242) for ow cytometry and IF was purchased from CST. An anti-Cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam. A puri ed anti-human CD3 mAb (OKT3, #317326) for cell incubation, and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for ow cytometry were purchased from BioLegend.
6
CD137 Expression Analysis in Tumor Samples
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RNAlater® was purchased from Ambion. TRIzol was purchased from Invitrogen. DEPC was purchased from Bio Basic Inc. The SYBR® PrimeScript® RT-PCR Kit was purchased from TaKaRa for two-step RT-PCR. PCR primers were designed by TaKaRa and synthesized by Yingjun Biotechnology Co., Ltd. An anti-CD137 rabbit mAb (#34549) used for IHC and IF and was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST. An agonistic anti-CD137 mAb (#79097) was purchased from BPS Bioscience. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST. Anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from the Proteintech group. MojoSort TM Magnet, MojoSort TM Human CD8 Nanobeads and MojoSort TM Human CD8 Cell Isolation Kit were purchased from BioLegend. An NF-κB p65 rabbit mAb (#8242) for ow cytometry and IF was purchased from CST. An anti-cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam. A puri ed anti-human CD3 mAb (OKT3, #317326) for cell incubation and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for ow cytometry were purchased from BioLegend.
7
Detailed Immunology Reagents Protocol
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RNAlater® was purchased from Ambion, USA. TRIzol was purchased from Invitrogen, USA. DEPC was purchased from Bio Basic Inc, Canada. The SYBR® PrimeScript® RT-PCR Kit was purchased from TaKaRa, Japan for two-step RT-PCR. PCR primers were designed by TaKaRa, Japan and synthesized by Yingjun Biotechnology Co., Ltd, China. An anti-CD137 rabbit mAb (#34549) used for IHC and IF and was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST, USA. An agonistic anti-CD137 mAb (#79097) was purchased from BPS Bioscience, USA. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST, USA. Anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from the Proteintech group, China. MojoSort TM Magnet, MojoSort TM Human CD8 Nanobeads and MojoSort TM Human CD8 Cell Isolation Kit were purchased from BioLegend, USA. An NF-κB p65 rabbit mAb (#8242) for ow cytometry and IF was purchased from CST, USA. An anti-cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam, England. A puri ed anti-human CD3 mAb (OKT3, #317326) for cell incubation and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for ow cytometry were purchased from BioLegend, USA.
8
Detailed Immunology Reagents Protocol
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RNAlater® was purchased from Ambion, USA. TRIzol was purchased from Invitrogen, USA. DEPC was purchased from Bio Basic Inc, Canada. The SYBR® PrimeScript® RT-PCR Kit was purchased from TaKaRa, Japan for two-step RT-PCR. PCR primers were designed by TaKaRa, Japan and synthesized by Yingjun Biotechnology Co., Ltd, China. An anti-CD137 rabbit mAb (#34549) used for IHC and IF and was purchased Cell Signaling Technology (CST, USA). An IHC detection reagent (HRP, rabbit, #8114) was purchased from CST, USA. An agonistic anti-CD137 mAb (#79097) was purchased from BPS Bioscience, USA. An anti-Foxp3 rabbit mAb (#12653) used for IHC was purchased from CST, USA. Anti-CD8 mouse antibody (#66868-1-Ig) for IHC and IF was purchased from the Proteintech group, China. MojoSort TM Magnet, MojoSort TM Human CD8 Nanobeads and MojoSort TM Human CD8 Cell Isolation Kit were purchased from BioLegend, USA. An NF-κB p65 rabbit mAb (#8242) for ow cytometry and IF was purchased from CST, USA. An anti-cytokeratin mouse mAb (#ab756) used for IHC was purchased from Abcam, England. A puri ed anti-human CD3 mAb (OKT3, #317326) for cell incubation and anti-CD45-PerCP (#368506), anti-CD3-FITC (#300406), anti-CD8-APC (#301014) and anti-CD137-APC (#309809) antibodies for ow cytometry were purchased from BioLegend, USA.
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