Barnstead e pure ultrapure water purification system

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Barnstead™ E-Pure™ Ultrapure Water Purification System is a water purification device that produces high-purity water. It utilizes a multi-stage filtration process to remove impurities and contaminants, resulting in ultrapure water suitable for various laboratory applications.

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Ultrapure water (169 citations) Water purification system (27 citations) Pure water (14 citations) Barnstead Ultrapure Water Purification System (4 citations) Barnstead E-pure (3 citations) Barnstead E-pure system (2 citations) E-Pure water (2 citations) E-pure system (2 citations)

9 protocols using barnstead e pure ultrapure water purification system

Phytotoxicity Evaluation of Mellein

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Sucrose, sodium nitrate, potassium chloride, dipotassium phosphate, magnesium sulfate, iron (II) sulfate, and agar were purchased from Fisher Chemicals (Fair Lawn, NJ, USA). Enantiomerically pure (R)-(-)-mellein purchased from Cayman Chemical (Ann Arbor, MI, USA) with a listed purity of ≥98%, which was confirmed by GC-MS analysis (Chen, 2017 (link)), was used as an LC-MS/MS standard and for phytotoxicity testing with soybean seedlings. 3-Nitropropionic acid for use as an LC-MS/MS standard was purchased from Sigma-Aldrich (Vienna, Austria). For culture medium preparation and phytotoxicity assays, reverse osmosis water was purified by a Barnstead E-Pure Ultrapure Water Purification System (Thermo Scientific, Marietta, OH, USA).
Reagents for LC-MS/MS analysis consisted of LC-MS grade ammonium acetate and glacial acetic acid purchased from Sigma-Aldrich (Vienna, Austria), LC-MS Chromasolv grade methanol purchased from Honeywell (Seelze, Germany), HiPerSolv Chromanorm HPLC gradient grade acetonitrile purchased from VWR Chemicals (Vienna, Austria), and reverse osmosis water purified using a Purelab Ultra system (ELGA LabWater, Celle, Germany).

Khambhati V.H., Abbas H.K., Sulyok M., Tomaso-Peterson M., Chen J, & Shier W.T. (2023). Mellein: Production in culture by Macrophomina phaseolina isolates from soybean plants exhibiting symptoms of charcoal rot and its role in pathology. Frontiers in Plant Science, 14, 1105590.

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Photochemical Water Oxidation Catalysis

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Unless indicated otherwise, chemicals were purchased from Sigma-Aldrich and used without further purification. These chemicals include benzoyl chloride (ACS reagent, 99%), 3-(N-morpholino)-propanesulfonic acid (MOPS) (≥99.5%), ammonium molybdate tetrahydrate (≥99.5%), nickel(ii) chloride (98%), potassium iodide (BioUltra, ≥99.5%), 4,4′,4′′,4′′′-(porphine-5,10,15,20-tetrayl)tetrakis(benzoic acid) (porphyrin, 75% dye content), Synperonic F-108 (Fluka Analytical), tetrabutylammonium hexafluorophosphate (≥99.0%), potassium chloride (Fisher Scientific, certified ACS), titanium(iv) oxysulfate solution (15 wt% in dilute sulfuric acid), acetonitrile (Extra dry, Acros Organics), hydrogen peroxide (50% aqueous solution), sulfuric acid (Fisher Scientific, certified ACS Plus), 18-crown-6 (≥99.0%), potassium dioxide. Fluorine-doped tin oxide (FTO) glass (Tech 7, thickness 2.2 mm, 2.5 cm × 2.5 cm) was purchased from Hartford Glass Co. BH4 was synthesized based on our prior report.21 (link) Deionized water was obtained via filtration through a Thermo Scientific Barnstead E-pure Ultrapure Water Purification System until greater than 18 MΩ cm in conductivity.

Sun J., Yu Y., Curtze A.E., Liang X, & Wu Y. (2019). Dye-sensitized photocathodes for oxygen reduction: efficient H2O2 production and aprotic redox reactions †Electronic supplementary information (ESI) available. See DOI: 10.1039/c9sc01626k. Chemical Science, 10(21), 5519-5527.

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Hafnium Tetrachloride Synthesis Protocols

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Hafnium(IV) tetrachloride (98%) was used as the Hafnium source. Acetone, acetonitrile, hexanes, methanol, and pentane were all HPLC Plus (>99.9%). Hydrochloric acid (35.0–37.0%), sulfuric acid (95.0–98.0%), Sodium chloride (>99%) and deuterated water (D₂O) were used as purchased from Sigma-Aldrich. N-Methyl formamide (99%) and sodium hydroxide pellets (98%) were purchased from Alfa Aesar. Poly(ethylene oxide) (MW 100 000) was purchased from Polysciences Inc. Propylene oxide (99.5%) and Trifluoroacetic acid (99.5%) were purchased from Acros Organics. Perfluorooctanoic acid (>98%) was purchased from TCI America. Ultrapure water (≥18.2 MΩ∙cm) used for synthesis and adsorption kinetics experiments was obtained from a Thermo Scientific Barnstead E-Pure Ultrapure water purification system.

Hussain F.A., Janisse S.E., Heffern M.C., Kinyua M, & Velázquez J.M. (2022). Adsorption of perfluorooctanoic acid from water by pH-modulated Brönsted acid and base sites in mesoporous hafnium oxide ceramics. iScience, 25(4), 104138.

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Preparation of Supramolecular Glycoconjugate

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SMG consists of 31 amino acids in the main peptide chain whose sequence is available elsewhere (38 (link)). A C18 fatty di-acid side chain is conjugated to the peptide backbone. The molecular weight of an SMG monomer is 4,114 Da. SMG was purchased from PolyPeptide in lyophilized powder form. The powder as received was dispersed into phosphate buffer (sodium phosphate dibasic dihydrate,

{Na}_{2} {HPO}_{4} \cdot 2 H_{2} O

, 8 mM) with addition of sodium chloride (NaCl, 140 mM). Ultrapure water (Barnstead™ E-Pure™ Ultrapure Water Purification System, Thermo Scientific, Massachusetts, USA) was used, with a resistivity of

18 .2 MΩ cm

, total organic carbon below 10 ppb. The pH was adjusted to 7.4. The batch solution was made to have a SMG concentration of 20 mg/mL.

Li Q., Tangry V., Allen D.P., Seibert K.D., Qian K.K, & Wagner N.J. (2024). Surface-mediated spontaneous emulsification of the acylated peptide, semaglutide. Proceedings of the National Academy of Sciences of the United States of America, 121(5), e2305770121.

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Synthesis and Characterization of Monodisperse Silica Particles

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Monodisperse silica particle were purchased from Pinfire -Gems & Colloids and characterized by electron microscopy in a FEI Quanta 600 FEG Environmental Scanning Electron Microscopy (ThermoFisher Scientific, Hillsboro, OR, USA). Acrylic acid (AA), azobisisobutyronitrile (AIBN), ethylene glycol dimethylacrylate (EGDMA), hydrofluoric acid (HF), and dimethylformamide (DMF) were purchase from Sigma-Aldrich (St. Louis, MO, USA). CHL, purity >95% was purchase from Key Organics (Bedform, MA, USA) while CHL analytical standard was obtained from Sigma-Aldrich (St. Louis, MO, USA). Ethanol 200 proof (anhydrous) was purchase from Decon Laboratories Inc. (King of Prussia, PA, USA).
Glass microscope slides with dimensions 1 × 13 × 76 mm were obtained from Fisher Scientific (Pittsburgh, PA, USA). Polymethylmethacrylate (PMMA) slides were purchased from ePlastic (San Diego, CA, USA) and cut into the same dimension as the glass slides. Ultra-pure water (18.2 MΩ•cm at 25 °C) was used in all experiments and obtained from a Thermo Scientific™ Barnstead™ E-Pure™ Ultrapure Water Purification System (Waltham, MA, USA).
All reactants and solvents were purchased as reagent grade and used without further purification.

Rossi E., Salahshoor Z., Ho K.V., Lin C.H., Errea M.I, & Fidalgo M.M. (2021). Detection of chlorantraniliprole residues in tomato using field-deployable MIP photonic sensors. Mikrochimica acta, 188(3).

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Ultrapure Water Preparation Protocol

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Water with a resistivity of 18.2 MΩ/cm was acquired from a Thermo Scientific Barnstead E-Pure Ultrapure Water Purification system. All reagents were purchased from Sigma Aldrich and used without additional purification.

Liz J.S., Anderes L., Sumner J.W., Massung R.F., Gern L., Rutti B, & Brossard M. (2000). PCR Detection of Granulocytic Ehrlichiae in Ixodes ricinus Ticks and Wild Small Mammals in Western Switzerland. Journal of Clinical Microbiology, 38(3), 1002-1007.

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Screen-Printed Carbon Sensors for Glucose Sensing

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Example 6

Screen-printed carbon sensors on PET substrates were obtained from Steven Label, Inc. (Santa Fe Springs, Calif.). The active area of the working electrode was defined by the deposited area of a Glucose-oxidizing catalyst, which was roughly 0.1 mm². A proprietary redox polymer used for Glucose oxidase (GOx) wiring and a proprietary flux-limiting membrane polymer were synthesized according to published procedures, and obtained from Nanosyn, Inc. (Santa Rosa, Calif.) and Regis Technologies, Inc. (Morton Grove, Ill.), respectively. Glucose oxidase (GOx, EC 1.1.3.4, activity 130 U/mg) from Aspergillus sp. II was obtained from Toyobo Co, Ltd. (Osaka, Japan). Poly(ethylene glycol) (400) diglycidyl ether (PEGDGE 400) and glyceryl triglycidyl ether was obtained from Polysciences, Inc. (Warrington, Pa.). Multi-walled carbon nanotubes (CNTs, OD 20-40 nm, length 10-20 μm) were obtained from MK Nano (Mississauga, Ontario, Canada). Glucose and the common chemicals used for buffer solutions were obtained from Sigma-Aldrich (St. Louis, Mo.). All aqueous solutions were made using >18.0 MΩ·cm⁻¹deionized water obtained from a Thermo Scientific Barnstead E-Pure ultrapure water purification system.

US11371957B2. Method and apparatus for analyte detection using an electrochemical biosensor (2022-06-28). ABBOTT DIABETES CARE INC. [US]. Inventors: Stephen M. Oja [US], Benjamin Feldman [US].

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Crystalline Thiamine Mononitrate Formulation

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Crystalline thiamine mononitrate (TMN) was purchased from Spectrum Chemical Mfg. Corp. (New Brunswick, NJ, USA). The polymers used in the study were pectin (PEC) from citrus peel with a ~61% degree of esterification and polyvinylpyrrolidone (PVP) with a molecular weight of 40,000, both of which were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). The RH conditions (reported here at 25 °C) were created in desiccators using saturated salt solutions of lithium chloride (LiCl, 11% RH) (EMD Millipore, Billerica, MA, USA) or sodium chloride (NaCl, 75% RH) (Sigma-Aldrich Inc.). HPLC grade trifluoroacetic acid (TFA) was purchased from Sigma-Aldrich Inc., and acetonitrile was purchased from Fisher Scientific Co., LLC (Pittsburgh, PA, USA). A Barnstead E-Pure ultrapure water purification system (ThermoScientific, Waltham, MA, USA) with a resistivity of ~17.5 MΩ·cm was used to deionize and purify all water used in this study.

Arioglu-Tuncil S., Voelker A.L., Taylor L.S, & Mauer L.J. (2020). Amorphization of Thiamine Mononitrate: A Study of Crystallization Inhibition and Chemical Stability of Thiamine in Thiamine Mononitrate Amorphous Solid Dispersions. International Journal of Molecular Sciences, 21(24), 9370.

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Thiamine Mononitrate and Chloride Characterization

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Two salt forms of thiamine were used in this study: thiamine mononitrate, C₁₂H₁₇N₄OS·NO₃ (TMN) (Spectrum Chemical Mfg. Corp., New Brunswick, NJ) and thiamine chloride hydrochloride, C₁₂H₁₇ClN₄OS·HCl (TClHCl) (Fisher Scientific, Fair Lawn, NJ). Nitric acid (HNO₃) (J.T. Baker, Center Valley, PA), hydrochloric acid (HCl) (Acros Organics, Fair Lawn, NJ), and sodium hydroxide (NaOH) (Sigma-Aldrich, St. Louis, MO) were used to adjust the pH of thiamine solutions. For use in high performance liquid chromatography (HPLC), HPLC grade acetonitrile and trifluoroacetic acid (TFA) were obtained from Fisher Scientific. All water used throughout the study was deionized and purified using a Barnstead E-pure ultrapure water purification system with a resistivity greater than 17.5 MΩ·cm at 25 °C (ThermoScientific, Waltham, MA).

Voelker A.L., Taylor L.S, & Mauer L.J. (2021). Effect of pH and concentration on the chemical stability and reaction kinetics of thiamine mononitrate and thiamine chloride hydrochloride in solution. BMC Chemistry, 15(1), 47.

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