After deparaffinization and rehydration, tissue sections were treated with EDTA, pH 9, in a steamer. Anti-Smad4 antibody (sc-7966; Santa Cruz Biotechnology, Dallas, TX) (1:20) was applied on tissue sections for a 1-hour incubation at room temperature in a humidity chamber. After Tris-buffered saline wash, the antigen–antibody binding was detected with the Bond polymer refine detection system (DS9800; Leica Microsystems, Buffalo Grove, IL) and 3,3′-diaminobenzidine tetra hydrochloride-positive chromogen (K3468; DAKO, Carpinteria, CA). Tissue sections were immersed briefly in hematoxylin for counterstaining and were covered with cover glasses. The University of Chicago clinical immunohistochemistry (IHC) laboratory completed staining for p53 (p53 Ab-6, clone DO-1, 1:200; Thermo Scientific) and p16 (p16 [JC8], sc-56330, 1:50; Santa Cruz Biotechnology).
Anti smad4 antibody sc 7966
Manufactured by Santa Cruz Biotechnology
The Anti-Smad4 antibody (sc-7966) is a research-use only product manufactured by Santa Cruz Biotechnology. It is a primary antibody that recognizes the Smad4 protein, a key regulator of the TGF-beta signaling pathway.
Automatically generated - may contain errors
2 protocols using anti smad4 antibody sc 7966
1
Immunohistochemical Analysis of Smad4, p53, and p16
2
Immunohistochemical Analysis of Smad4, p53, and p16
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After deparaffinization and rehydration, tissue sections were treated with EDTA, pH 9, in a steamer. Anti-Smad4 antibody (sc-7966; Santa Cruz Biotechnology, Dallas, TX) (1:20) was applied on tissue sections for a 1-hour incubation at room temperature in a humidity chamber. After Tris-buffered saline wash, the antigen–antibody binding was detected with the Bond polymer refine detection system (DS9800; Leica Microsystems, Buffalo Grove, IL) and 3,3′-diaminobenzidine tetra hydrochloride-positive chromogen (K3468; DAKO, Carpinteria, CA). Tissue sections were immersed briefly in hematoxylin for counterstaining and were covered with cover glasses. The University of Chicago clinical immunohistochemistry (IHC) laboratory completed staining for p53 (p53 Ab-6, clone DO-1, 1:200; Thermo Scientific) and p16 (p16 [JC8], sc-56330, 1:50; Santa Cruz Biotechnology).
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