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Non targeting smart pool sirna

Manufactured by Horizon Discovery
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Non-targeting smart pool siRNA is a collection of small interfering RNA (siRNA) sequences designed to have no known target in the human, mouse, or rat genome. It is used as a control in RNA interference (RNAi) experiments to help distinguish specific gene knockdown effects from non-specific effects.

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Lab products found in correlation

Sirna pool, by Horizon Discovery (2 mentions) Oligofectamine reagent, by Thermo Fisher Scientific (2 mentions) Sc 37455, by Santa Cruz Biotechnology (2 mentions) Sc 29827, by Santa Cruz Biotechnology (2 mentions) L 003506 00, by Horizon Discovery (2 mentions) Oligonucleotide mix, by Horizon Discovery (2 mentions) Oligonucleotide targeting, by Horizon Discovery (2 mentions) Non targeting sirna control sc 37007, by Horizon Discovery (2 mentions) On targetplus smartpool sirna, by Horizon Discovery (2 mentions) On targetplus smartpool sirna against mouse, by Horizon Discovery (1 mentions)

5 protocols using non targeting smart pool sirna

1

Targeting DNA Repair Proteins with siRNA

Cited 1 time
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siRNAs targeting human 53BP1 (sc-37455) and BRG1 (sc-29827) and TOP2A (sc-36695) were obtained from Santa Cruz Biotechnology. siRNA pools targeting MDC1 (L-003506-00) and PICH (L-031581-01) were obtained from Dharmacon. Oligonucleotide mix targeting the 3′ UTR of endogenous human TOPBP1 (Dharmacon) contained the following oligos: 5′-GUAAAUAUCUGAAGCUGUAUU-3′, 5′-GCACAAGGUUUAAUGAGGAUU-3′, 5′-GCUGUAGCUUAGUGGAAAUUU-3′. Oligonucleotide targeting the 3′ UTR of BLM (Dharmacon) was as follows: 5′-GCUAGGAGUCUGCGUGCGAUU-3′. siRNA targeting TOP2B was 5′-UCGGGCUAGGAAAGAAGUAAA-3′50 (link):. Santa Cruz Biotechnology non-targeting siRNA control (sc-37007) or Dharmacon non-targeting smart pool siRNA (D-00180-10-20) were used as control siRNAs where appropriate. Oligonucleotides were transfected into human cells using Oligofectamine reagent (Life Technologies), according to the manufacturer’s instructions.
Broderick R., Nieminuszczy J., Blackford A.N., Winczura A, & Niedzwiedz W. (2015). TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution. Nature communications, 6, 6572.
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2

siRNA-mediated Sqstm1 and Flna Silencing

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ON-TARGETplus SMARTpool siRNA against mouse Sqstm1 (L-047628-01), Flna (L-058520-01) and nontargeting SMARTpool siRNA (D-001810-04) were purchased from Dharmacon. Final siRNA concentrations of 100 nM were used for 96 h for silencing, and transfections were carried out using Lipofectamine 2000 as per company instructions.
Hewitt G., Carroll B., Sarallah R., Correia-Melo C., Ogrodnik M., Nelson G., Otten E.G., Manni D., Antrobus R., Morgan B.A., von Zglinicki T., Jurk D., Seluanov A., Gorbunova V., Johansen T., Passos J.F, & Korolchuk V.I. (2016). SQSTM1/p62 mediates crosstalk between autophagy and the UPS in DNA repair. Autophagy, 12(10), 1917-1930.
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3

Knockdown of p21 in RRCL

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Knockdown of p21 was achieved using ON-TARGET plus SMART pool siRNA containing a mixture of 4 siRNAs designed to specifically target CDKN1A (Dharmacon, Lafayette, CO). Non-targeting SMART pool siRNA (Dharmacon) was used as a negative control. Efficient knockdown of p-21 was confirmed by Western blotting. Once conditions were optimized, RRCL were transfected with 2 μg of siRNA targeting CDKN1A or control using an Amaxa Nucleofector (Lonza laboratories, Anaheim CA) and cells were incubated with BTZ (0– 25 nM) for an additional 24 or 48 hrs. Differences in cell viability, senescence and mitotic index were determined by ATP quantification, β-galactosidase and Wright-Giemsa staining respectively.
Gu J.J., Kaufman G.P., Mavis C., Czuczman M.S, & Hernand ez-Ilizaliturri F.J. (2016). Mitotic catastrophe and cell cycle arrest are alternative cell death pathways executed by bortezomib in rituximab resistant B-cell lymphoma cells. Oncotarget, 8(8), 12741-12753.
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4

HKII Silencing for Metabolic Regulation

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We silenced HKII using siRNA interference by using ON-TARGET plus SMART pool siRNA containing a mixture of 4 siRNAs designed to specifically target HKII (Dharmacon, Lafayette, CO). Non-targeting SMART pool siRNA (Dharmacon) was used as a negative control. Efficient knockdown of HKII was confirmed by Western blotting. Once conditions were optimized, RSCL, RRCL or TRCL were transfected with siRNA targeting HKII or control using an Amaxa Nucleofector (Lonza laboratories, Anaheim CA) and cells were incubated for an additional 48 hours. Differences in glucose consumption and cell viability were determined as previously described.
Gu J.J., Singh A., Xue K., Mavis C., Barth M., Yanamadala V., Lenz P., Grau M., Lenz G., Czuczman M.S, & Hernandez-Ilizaliturri F.J. (2017). Up-regulation of hexokinase II contributes to rituximab-chemotherapy resistance and is a clinically relevant target for therapeutic development. Oncotarget, 9(3), 4020-4033.
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5

Targeting DNA Repair Proteins with siRNA

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
siRNAs targeting human 53BP1 (sc-37455) and BRG1 (sc-29827) and TOP2A (sc-36695) were obtained from Santa Cruz Biotechnology. siRNA pools targeting MDC1 (L-003506-00) and PICH (L-031581-01) were obtained from Dharmacon. Oligonucleotide mix targeting the 3′ UTR of endogenous human TOPBP1 (Dharmacon) contained the following oligos: 5′-GUAAAUAUCUGAAGCUGUAUU-3′, 5′-GCACAAGGUUUAAUGAGGAUU-3′, 5′-GCUGUAGCUUAGUGGAAAUUU-3′. Oligonucleotide targeting the 3′ UTR of BLM (Dharmacon) was as follows: 5′-GCUAGGAGUCUGCGUGCGAUU-3′. siRNA targeting TOP2B was 5′-UCGGGCUAGGAAAGAAGUAAA-3′50 (link):. Santa Cruz Biotechnology non-targeting siRNA control (sc-37007) or Dharmacon non-targeting smart pool siRNA (D-00180-10-20) were used as control siRNAs where appropriate. Oligonucleotides were transfected into human cells using Oligofectamine reagent (Life Technologies), according to the manufacturer’s instructions.
Broderick R., Nieminuszczy J., Blackford A.N., Winczura A, & Niedzwiedz W. (2015). TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution. Nature communications, 6, 6572.
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