Plan apochromat vc pfs 1.4 na oil immersion objective

Live imaging was performed as described previously (Carvalho et al., 2018 (link)). Dechorionated stage 15 embryos were mounted on their ventral side on glass-bottomed culture dishes (MatTek) with embryo glue (double-sided tape diluted in heptane) and covered with halocarbon oil 27 (Sigma-Aldrich). Embryos were wounded as described above for the wounding assay except that the laser power was lower in order to inflict smaller wounds that are able to close during the imaging procedure.
Time-lapse microscopy of transgenic embryos was performed at 25°C on a Nikon/Andor Revolution XD spinning-disk confocal microscope with a 512 EMCCD camera (iXon 897) with a 60× Plan Apochromat VC PFS 1.4 NA oil-immersion objective or a 60× Plan Apochromat VC PFS 1.2 NA water-immersion objective (Nikon) and using the iQ software.
Individual z slices with a step size of 0.28 µm, (Figs 2, 3 and 4; Figs S1 and S3I–J), 0.36 µm (Fig. 7, Fig. S2) or 0.5 µm (Figs 5 and 6; Fig. S3A,B,E,F), were acquired for a single time point or every 30 s, 2 min, 2.5 min or 10 min for 30–160 min. For F-actin, myosin, E-cad and Rho effectors imaging, Z stacks were acquired with frame averaging of 2. For mitochondrial morphology quantification, images were acquired with frame averaging of 4.