Hy 101193

EEC cells were treated with 0, 12.5 or 25 μM heme (H8130, Solarbio, Beijing, China), and the relevant cells were collected after 48 h. EEC cells were treated with HO-1 inhibitor (Zinc Protoporphyrin, 5 μM, HY-101193, MedChem Express, NJ, USA) or Nrf2 inhibitor (ML385, 5 μM, HY-100523, MedChem Express, NJ, USA), and the relevant cells were collected after 24 h.

HASMCs were purchased from China Center for Type Culture Collection (CCTCC, GPC0113). Cells were cultured in Dulbecco Modified Eagle medium (DMEM, Hyclone) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Hyclone) in an incubator with 5% CO₂ at 37°C. After reaching a density of 70–80%, cells were treated with erastin (HY-15763, MedChemExpress), zVAD-fmk (HY-16658B, MedChemExpress), necrosulfonamide (HY-100573, MedChemExpress), 3-Methyladenine (3MA, HY-19312, MedChemExpress), Zinc Protoporphyrin (ZnPP, HY-101193, MedChemExpress), hemin (HY-19424, MedChemExpress) and Deferoxamine (DFO, Ba33112, MedChemExpress) for 24 h, respectively. Human monocytic cell line THP-1 was acquired from CCTCC (GDC0100) and cultured in Roswell Park Memorial Institute 1640 medium (RPMI 1640; Hyclone) supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Hyclone) in an incubator with 5% CO₂ at 37°C.