Beyoclick edu cell proliferation detection kit

The proliferation was detected by BeyoClick™ EdU cell proliferation detection kit (#C0085S, Beyotime, Shanghai, China) according to the manufacturer’s protocol. Under fluorescence microscope (200×) the nucleus was dyed blue, and EdU-positive cells were dyed red. To measure cell proliferation quantitatively, the proportion of EdU-positive cells was calculated.

The EdU assay kit used was BeyoClick™ EdU Cell Proliferation Detection Kit (Beyotime, China). Briefly, the logarithmically growing target cells were collected and seeded into 35mm glass bottom dishes (Biosharp, China). After the termination of the incubation, the EdU working solution was prepared and labelled as described in the instructions. The confocal microscope was used to photograph images, and Image J was used to count EdU-positive cells.

The logarithmically growing target cells were collected and seeded into 35 mm glass bottom dishes (Biosharp, China) (500 cells were seeded per well). Subsequently, cells were incubated with Edu by adding EdU to the culture medium for 2 h. After incubation the confocal microscope (Olympus, Japan) was used to photograph them, and Image J was used to count EdU-positive cells. In this experiment, more than 50 cells in the field of view were randomly selected for counting, and statistical analysis was performed from five different regions, and three independent experiments were carried out. BeyoClick™ EdU Cell Proliferation Detection Kit (Beyotime Biotechnology, China) was used for the EdU assay.