Onvansertib

Antibodies and reagents were acquired from designated suppliers:
TRIM26 (ab188017, Abcam), TRIM26 (27013-1-AP, Proteintech), TRIM26 (sc-393832, Santa Cruz Biotechnology), GPX4 (ab125066, Abcam), GPX4 (sc-166570, Santa Cruz Biotechnology), PLK1 (ab189139, Abcam), p-PLK1 (ab155095, Abcam), β-actin (ab8226, Abcam), K48-ubiquitin (ab140601, Abcam), K63-ubiquitin (12930, Cell Signaling Technology), anti-Flag (66008, Proteintech), anti-Myc (16286, Proteintech), p-S/T (61 G, abmart), p-S/T (612549, BD Biosciences), Anti-His (66005, Proteintech), anti-Flag (14793, Cell Signaling Technology), anti-Myc (2276, Cell Signaling Technology), Anti-His (12689, Cell Signaling Technology), anti-HA (3724, Cell Signaling Technology),
MDA (ab27642, Abcam), 4-HNE (ab48506, Abcam), MG132 (S1748, Beyotime), cycloheximide (HY12320, MedChemExpress), Chloroquine (HY-17589A, MedChemExpress), Onvansertib (HY-15828, MedChemExpress), MLN0905 (HY-15155, MedChemExpress), Recombinant Human PLK1 (ab271716, Abcam).

Onvansertib (purity 99.7%) was purchased from Med Chem Express (Monmouth Junction, NJ, USA). HPLC grade methanol and acetonitrile were obtained from Merck Life Science Co. Ltd. (Shanghai, China). Formic acid and phosphate-buffered saline were bought from Sigma Aldrich Trading Co., Ltd (Shanghai, China). Nicotinamide adenine dinucleotide phosphate (NADPH) was purchased from Roche Diagnostics GmbH (Mannheim, Germany). Ultrapure deionized water was obtained by a Milli-Q water system (Millipore, Burlington, MA, USA). The cell lines were purchased from the American Type Culture Collection (Manassas, VA, USA). PLK1 kinase (Batch NO. 05–157) was purchased from CarnaBio (Natick, MA, USA). The rat and human liver microsomes were purchased from Corning (Tewksbury, MA, USA). The HCT116 cell line was obtained from National Collection of Authenticated Cell Cultures (Shanghai, China). The NCI-H2030 cell line was obtained from the American type culture collection.