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Hiseq4000 ngs sequencer

Manufactured by Illumina
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The HiSeq4000 is a next-generation sequencing (NGS) instrument manufactured by Illumina. It is designed to perform high-throughput DNA sequencing. The HiSeq4000 utilizes Illumina's proprietary sequencing-by-synthesis technology to generate sequencing data.

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Lab products found in correlation

Dna sample preparation kit, by Illumina (1 mentions) Set a to d, by Illumina (1 mentions) Nextera xt, by Illumina (1 mentions)

2 protocols using hiseq4000 ngs sequencer

1

High-throughput Nextera XT Library Prep

Cited 2 times
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Sequencing libraries were prepared using the standard Illumina Nextera XT. DNA Sample Preparation kit (Illumina, #FC-131-1096) and the combination of 384 Combinatorial Dual Indexes (Illumina- Set A to D, #FC-131-2001 to FC-131-2004). Using the Mosquito liquid handling robot, the Nextera XT chemistry was miniaturized126 (link),127 (link) (Supp. Dataset 10–14 and Supp. Information). All the final 384-pooled libraries were sequenced using Illumina HiSeq4000 NGS sequencer in a paired-end—150 bases length.
Richter M.L., Deligiannis I.K., Yin K., Danese A., Lleshi E., Coupland P., Vallejos C.A., Matchett K.P., Henderson N.C., Colome-Tatche M, & Martinez-Jimenez C.P. (2021). Single-nucleus RNA-seq2 reveals functional crosstalk between liver zonation and ploidy. Nature Communications, 12, 4264.
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2

Single-cell RNA Sequencing Protocol

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All the final 384-pooled libraries were sequenced using Illumina HiSeq4000 NGS sequencer in a paired-end 150 bases length. Each 384-pooled library was sequenced in one lane leading on average to ~1 million reads per nuclei.
Richter M.L., Deligiannis I.K., Danese A., Lleshi E., Coupland P., Vallejos C.A., Colomé‐Tatché M., & Martinez‐Jimenez C.P. (2020). “Single-nucleus RNA-seq2 reveals a functional crosstalk between liver zonation and ploidy”.
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