Larval eye discs were fixed for 10 min in 4% paraformaldehyde. Primary antibodies used were: mouse anti-α-synuclein (BD Transduction, 1:500) and rabbit anti-Nedd4 (Abcam, 1:500). The Cy5 conjugated (Jackson ImmunoResearch, 1:1000) secondary antibody was used. Samples were imaged using a Zeiss LSM 510 META confocal microscope.
Rabbit anti nedd4
Manufactured by Abcam
Rabbit anti-Nedd4 is a primary antibody that specifically binds to the Nedd4 protein. Nedd4 is an E3 ubiquitin-protein ligase that plays a role in the ubiquitination and subsequent proteasomal degradation of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti α synuclein, by BD (2 mentions)
Lsm 510 meta, by Zeiss (2 mentions)
Ecl detection reagent, by Cytiva (1 mentions)
Rabbit anti actin, by Merck Group (1 mentions)
Complete protease inhibitor cocktail, by Merck Group (1 mentions)
Mouse anti tubulin, by Developmental Studies Hybridoma Bank (1 mentions)
2 protocols using rabbit anti nedd4
1
Immunofluorescence Imaging of Larval Eyes
2
Protein Expression Analysis in Genotypes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For each genotype 5 male adult heads were homogenized in lysis buffer (150 mM NaCl, 10 mM Tris pH7.4, 1% Triton-X, 0.1% SDS and complete protease inhibitor cocktail, SIGMA). Samples were quantified and loaded on a NuPAGE 10–12% Bis–Tris gel (Invitrogen). The following primary antibodies were: mouse anti-tubulin (Developmental Studies Hybridoma Bank, 1:1000), mouse anti-α-synuclein, Syn1 (BD Transduction, 1:400), rabbit anti-Nedd4 (Abcam, 1:5000), and rabbit anti-actin (Sigma, 1:1000). Blots were visualized using HRP-conjugated secondary antibodies and the ECL Detection Reagent (Amersham).
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