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Automated chemistry analyzer

Manufactured by Beckman Coulter
Sourced in United States, Germany

The Automated Chemistry Analyzer is a laboratory instrument designed to perform a variety of clinical chemistry tests. It automates the process of measuring the concentration of specific analytes in biological samples, such as blood, urine, or other bodily fluids. The core function of this analyzer is to provide accurate and reliable test results through a streamlined and efficient analytical process.

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9 protocols using automated chemistry analyzer

1

Plasma Biochemical Analysis Protocol

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The ALT and creatinine levels in the plasma sample were determined by an automated chemistry analyzer (Beckman Coulter, Brea, CA, USA) [19 (link)].
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2

Comprehensive HBV Biomarker Analysis

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Blood biochemical parameters, including ALT, were measured using an automated chemistry analyzer (Beckman Coulter). Serum HBV DNA levels were quantified using real-time PCR (Shanghai Kehua Bio-Engineering Co., Ltd.) with a lower limit of detection of 500 IU/ml. Quantitative serum HBsAg levels and the presence of HBeAg and anti-HBe antibody were measured using the Abbott Architect immunoassay system (Abbott Laboratories). HBV genotypes were determined by direct sequencing of the pre-S/S gene and comparison with the reference sequences in GenBank (NCBI).
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3

Streptozotocin-Induced Diabetic Rat Model

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The following drugs, chemicals, and instruments were used in the study. Streptozotocin (Fisco Research laboratories, India), glibenclamide (Julphar pharmaceuticals, Ethiopia), citric acid (Lab tech chemicals, India), ascorbic acid (Blulux Labratories, India), n-hexane (Loba chemie, India), chloroform (Aristar, England), sodium hydroxide (BluluxLabratories, India), DPPH (Sigma-Aldrich, Germany), sodium citrate (Lab tech chemicals, India), Methanol (Nice chemicals, India), 5% glucose solution (Reyoung pharmaceuticals, China), What man filter paper No.1, test tube, beakers, funnels, measuring cylinder, glass rod, spatula, pipettes, gavages (oral feeding syringes), Syringes (1 mL, 3 mL, and 5 mL) with needles, desiccators, digital analytical balance (EPH-400 Abron Exports), pH meter (Bante Instruments, UK), Sensor Card glucometer and strip (Alliance international, Taiwan), Lyophilizer (Labfreez, China), Hot air oven (Medit-Medizin Technik, Germany), Automated chemistry analyzer (Beckman coulter, Germany), UV-spectrophotometer (Agilent Technologies, Malaysia), rotary evaporator (Hamato, Japan), Tween-80 (Avishkar Lab Tech chemicals, India). All chemicals used were of analytical grade.
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4

Metabolic Parameters in Animal Study

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Fasting blood glucose concentration was determined using a commercial kit (Allmedicus, gyeonggi-do, Republic of Korea) at week 8. Fasting serum insulin (Shibayagi Co. Ltd, Gumma Pref, Japan) was also measured using a commercial kit. Serum lipids including triglyceride (TG), total cholesterol (TC), and LDL-cholesterol were determined using an Automated Chemistry Analyzer (Beckman Coulter Inc, Brea, Cal, USA).
Homeostasis model assessment of insulin resistance (HOMA-IR) was calculated as blood glucose (mM) * insulin concentration (mU/L)/ 22.5, utilizing serum obtained at the time the animals were euthanized [27 (link)28 (link)].
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5

Liver Enzyme Analysis in Blood

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At the end of the experiments, blood was collected and centrifuged (2000× g, 20 min). Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were analyzed using an automated chemistry analyzer (Beckman Coulter Inc., Brea, CA, USA) according to the manufacturer’s instructions.
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6

Plasma Fibrinogen and Serum Albumin Measurement

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Venous blood samples from fasting patients were obtained within 24 hours after admission. Plasma fibrinogen was measured on ACL‐Top 700 LAS (Werfen, USA) with an automated clot rate assay as previously described.
16 (link) Serum albumin was tested with an automated chemistry analyzer (Beckman, Japan). All measurements were performed by laboratory personnel with standard biochemical techniques in the clinical laboratory of the Second Hospital of Hebei Medical University. The intra‐ and interassay coefficients of variation were 1.8% and 3.2%, and 1.5% and 1.6%, respectively. We performed all measurements blind to clinical outcomes.
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7

Antioxidant Evaluation of Natural Compounds

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DPPH (Sigma-Aldrich, Germany), sodium citrate (Lab tech chemicals, India), methanol (Nice chemicals, India), 5% glucose solution (Reyoung pharmaceuticals, China), Whatman filter paper No.1, test tube, beakers, funnels, measuring cylinder, glass rod, spatula, pipettes, gavages (oral feeding syringes), Syringes (1 mL, 3 mL, and 5 mL) with needles, desiccators, digital analytical balance (EPH-400 Abron Exports), pH meter (Bante Instruments, UK), Hot air oven (Medit-Medizin Technik, Germany), Automated chemistry analyzer (Beckman Coulter, Germany), UV-spectrophotometer (Agilent Technologies, Malaysia), rotary evaporator (Hamato, Japan), Tween-80 (Avishkar Lab Tech chemicals, India), Streptozotocin (Fisco Research laboratories, India), glibenclamide (Julphar pharmaceuticals, Ethiopia), ascorbic acid (Blulux Labratories, Inida), Sensor Card glucometer and strip (Alliance international, Taiwan), Lyophilizer (Labfreez, China), ethyl acetate (Loba chemie, India), chloroform (Aristar, England), sodium hydroxide (Blulux Laboratories, India).
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8

Liver Function Markers Evaluation

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Blood samples were collected from the abdominal aorta of the liver transplant models, and serum levels of liver function markers, aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TBIL) (Nangjing Jiancheng Bioengineering Institute, Nanjing, China) were detected using an automated chemistry analyzer (Beckman, CA, USA).
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9

Plasma and Serum Extraction from Blood

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Whole blood was collected from the abdominal aorta and immediately transferred to heparinized tubes as previously described [26] . The heparinized and nonheparinized blood was centrifuged at 3, 000 × g for 20 minutes using a bench top centrifuge (Anke TGL-16B) to remove blood cells and recover plasma and serum, which were immediately stored at -20°C until further use. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of the different animal groups were detected by an automated chemistry analyzer (Beckman Coulter).
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