Gct premier

¹H NMR and ¹³C{¹H} NMR high-resolution nuclear magnetic resonance spectra were recorded on a Varian Mercury Plus 400 (400 MHz/101 MHz) or a Varian Unity Inova 500 (500 MHz/126 MHz) spectrometer at room temperature. Chemical shifts are reported relative to TMS (δ 0.00) or CDCl₃ (δ 7.26) for ¹H NMR and CDCl₃ (δ 77.2) for ¹³C{¹H} NMR. IR spectra were recorded as thin films (PerkinElmer Spectrum 100 FT-IR Spectrometer). High-resolution mass spectra were obtained on a Thermo-Electron LTQ-FT 7T Fourier transform ion cyclotron resonance (FT-ICR) spectrometer with an atmospheric pressure chemical ionization (APCI) source with direct infusion run in positive ion mode at 5 kV. Additional accurate mass measurement analyses were conducted on either a Waters GCT Premier, time-of-flight, GCMS with electron ionization (EI), or an LCT Premier XE, time-of-flight, LCMS with electrospray ionization (ESI). Samples were taken up in a suitable solvent for analysis. The signals were mass measured against an internal lock mass reference of perfluorotributylamine (PFTBA) for EI-GCMS, and leucine enkephalin for ESI–LCMS. Waters software calibrates the instruments, and reports measurements, by use of neutral atomic masses. The mass of the electron is not included.

¹H, ¹³C{¹H} and ³¹P{¹H} NMR spectra were recorded on Bruker AV400PAS and AV300PAS (Bruker, Karlsruhe, Germany) instruments. ¹H and ¹³C chemical shifts (δ, ppm) were measured relative to residual resonances of solvents. High-resolution mass spectra were recorded using GCT Premier (Waters, Milford, MA, USA) and UPLC Xevo G2 Q TOF (Waters, Milford, MA, USA) mass spectrometers for chemical ionization and electrospray ionization, respectively.