Hdac1

Manufactured by Santa Cruz Biotechnology

Sourced in United States, Germany, China

HDAC1 is a histone deacetylase enzyme that removes acetyl groups from lysine residues on histone proteins, leading to chromatin compaction and transcriptional repression.

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Lab products found in correlation

Close spelling variants of this product

HDAC1 siRNA (2 citations) Anti-HDAC1 antibody (4 citations) HDAC1 (H-51) (6 citations) Anti-HDAC1 (H-51, (2 citations) Anti-HDAC1 (27 citations) HDAC1 antibody (2 citations) HDAC1 (sc-7872), (4 citations) HDAC1 (sc-29344-V) (2 citations) Anti-HDAC1 (sc-7872; (4 citations) Rabbit anti-HDAC1 (2 citations)

72 protocols using hdac1

Isolation and Analysis of C/EBPα Complexes

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This procedure was used for the isolation of C/EBPα. Nuclear extracts were isolated from livers as described earlier (Timchenko et al., 1997) and fractionated by size‐exclusion column SEC400 (HPLC, HR; Bio‐Rad Laboratories Inc, Hercules, CA USA). The detailed procedure for the analysis of C/EBPα complexes is described in our previous papers (Wang et al., 2001, 2004). Briefly, gel filtration fractions were loaded on denaturing gradient (4‐20%) PAAG, blotted onto membrane, and probed with antibodies to C/EBPα (14AA), HDAC1, HP1α, and Brm (Santa Cruz Biotechnology, Dallas, Texas+9), to detect C/EBPα complexes, C/EBPα was immunoprecipitated from each fraction, and IPs were probed with antibodies to HDAC1.

Guillory B., Jawanmardi N., Iakova P., Anderson B., Zang P., Timchenko N.A, & Garcia J.M. (2017). Ghrelin deletion protects against age‐associated hepatic steatosis by downregulating the C/EBPα‐p300/DGAT1 pathway. Aging Cell, 17(1), e12688.

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Immunoblot Analysis of Key Proteins

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Western blot was done as previously described[20 (link)]. Antibodies specific for the following targets were from Santa Cruz (Heidelberg, Germany): BAX, histone deacetylase 2 (HDAC2), HDAC1, and p53. Anti-survivin was obtained from Novus Biologicals (Cambridge, United Kingdom). HSP90 antibody was provided by Enzo Life Sciences (Lörrach, Germany).

Gock M., Mullins C.S., Bergner C., Prall F., Ramer R., Göder A., Krämer O.H., Lange F., Krause B.J., Klar E, & Linnebacher M. (2018). Establishment, functional and genetic characterization of three novel patient-derived rectal cancer cell lines. World Journal of Gastroenterology, 24(43), 4880-4892.

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Quercetin Modulates Epigenetic Mechanisms

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Quercetin (> 98% pure) was obtained from Sigma Chemical Co. Antibodies used were as follows: DNMT1, STAT3, HDAC1, HDAC2, acetylated H4 (Ser 1/Lys5/Lys 8/Lys12) actin, DAPK1, BCL2L11, and control shRNA (h) lentiviral particles from Santa Cruz Biotechnology. DNMT3a and p-STAT3 were from Cell Signalling Technology. Acetylated H3 (Lys 9/Lys 18/Lys 23/Lys 27), H3, and H4 were from Abcam Inc. Anti-rabbit, anti-mouse, and anti-goat per-oxidase-conjugated antibodies were from KPL, Inc. DAPI, Alexa Fluor 555, and Alexa Fluor 488 molecular probes were from Invitrogen. The FITC–Annexin V Apoptosis Detection Kit I was from BD Pharmingen.

Alvarez M.C., Maso V., Torello C.O., Ferro K.P, & Saad S.T. (2018). The polyphenol quercetin induces cell death in leukemia by targeting epigenetic regulators of pro-apoptotic genes. Clinical Epigenetics, 10, 139.

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Th2 Cell Modulation in Allergy

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The antibodies of HDAC1, pSTAT6, p300, pp300, STAT3, pSTAT3, H3K4ac, RNA polymerase II, shRNA kits of p300 and STAT3 were from Santa Cruz Biotech (Santa Cruz, CA). The fluorochrome-labeled antibodies of Foxp3, CD4, IL-10, CD19 and IgE were from BD Biosciences (Franklin Lakes, NJ). The biotinylated IgE antibody was from Abcam (Cambridge, MA). Magnetic cell sorting kits were from Miltenyi Biotech (San Diego, CA). The house dust mite vaccine was from Wowu Biotech (Hangzhou, China). Clostridium butyricum was from Shenzhen Kexing Biotech (Shenzhen, China). The Der p 1 protein was from Dr. Zhijiang Liu (Shenzhen University, China). PCI-32765 was purchased from Chem Blink (Shanghai, China). Reagents for real time RT-PCR and Western blotting were from Invitrogen (Carlsbad, CA). Protein G, ChIP kit and butyrate sodium were from Sigma Aldrich (St. Louis., MO).

Liao H.Y., Tao L., Zhao J., Qin J., Zeng G.C., Cai S.W., Li Y., Zhang J, & Chen H.G. (2016). Clostridium butyricum in combination with specific immunotherapy converts antigen-specific B cells to regulatory B cells in asthmatic patients. Scientific Reports, 6, 20481.

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Investigating Cell Death Pathways

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The reagents and sources were as follows: Con A, LPS from Escherichia coli O111:B4, and N-galactosamine (GalN) (Sigma-Aldrich); TNF (Peprotech); α-galactosyl ceramide (α-GalCer, KRN7000) and Mdivi-1 (Enzo Life Sciences); Nec-1s (7-Cl-O-Nec-1, BioVision); FK 506 (LC Labs); cycloheximide (Calbiochem); phospho-ASK1(sc-109911, 1:500 dilution), NFATc2 (sc-7296, 1:1000 dilution), RIPK1 (sc-7881, 1:1000 dilution), RIPK3 (sc-47364, 1:1000 dilution), and HDAC1 (sc-7872, 1:500 dilution) antibodies (Santa Cruz Biotechnology); anti-GAPDH antibody (MAB374, 1:5000 dilution, Chemicon); phospho-p38α (#9211), phospho-JNK (#9251), phospho-ERK (#9101), and phospho-Drp1 (Ser637, #4867) antibodies (1:1000 dilution, Cell Signaling Technology); anti-MLKL antibody (AP14272b, 1:1000 dilution, Abgent); anti-Fas (clone Jo2, #554254, 5 μg per mouse, BD Pharmingen); CD3-FITC (#11-0032, 0.25 μg per 10⁵ cells), CD4-PerCP/Cy5.5 (#35-0042, 0.125 μg per 10⁵ cells), and CD69-PE antibodies (#1-0691, 0.25 μg per 10⁵ cells) (eBioscience); and MitoSOX RED (Molecular Probes). APC-mCD1d/PBS57 ligand tetramers were generously provided by the National Institute of Allergy and Infectious Disease MHC Tetramer Core Facility (Atlanta, GA, USA).

Kang Y.J., Bang B.R., Han K.H., Hong L., Shim E.J., Ma J., Lerner R.A, & Otsuka M. (2015). Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling. Nature Communications, 6, 8371.

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Cell Lysis and E2F1/HDAC1 Immunoprecipitation

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Cells were grown to about 70% confluency, then washed twice with 1xPBS, and collected in CHAPS buffer (25 mM HEPES, 2 mM EGTA, 2.5 mM MgCl2 and 0.3% CHAPS). After 20 min of incubation on ice and 10 min of centrifugation at 4°C, 500 μg of Lysates were pre-cleared with 30 μl Protein A/G Agarose beads (Invitrogen) for 2h and supernatants were collected. IPs were performed by mixing supernatants with 3 μg of E2F1 (Santa Cruz) or HDAC1 (Santa Cruz) antibodies or IgG (Abcam), together with 30 μl of Protein A/G Agarose beads, for overnight at 4°C. After 3 washes of PBS buffer, the immuno-complexes were eluted by 10 min boiling in 2x SDS loading buffer.

Wu M., Seto E, & Zhang J. (2015). E2F1 enhances glycolysis through suppressing Sirt6 transcription in cancer cells. Oncotarget, 6(13), 11252-11263.

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Immunoblotting for Chromatin Remodeling Proteins

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Rabbit polyclonal CHD5 antibody and goat primary polyclonal antibodies for MTA-1, MTA-2, RBBP4, RBBP7, histone deacetylase (HDAC)1, HDAC2, MBD2 and MBD3 were from Santa Cruz Biotechnology. CHD4 antibody was from Bethyl Laboratories. Polyclonal antibodies for HDAC1, HDAC2, RBBP7, RBBP4/7 and MDB3 were from Cell Signaling Technology. GATAD2A antibody was from Upstate Biotechnology. Tagged V5–His was from Invitrogen. Secondary antibodies were from GE Healthcare Life Sciences and Santa Cruz.

Kolla V., Naraparaju K., Zhuang T., Higashi M., Kolla S., Blobel G.A, & Brodeur G.M. (2015). The tumour suppressor CHD5 forms a NuRD-type chromatin remodelling complex. The Biochemical journal, 468(2), 345-352.

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Comprehensive Antibodies Analysis Protocol

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Antibodies used were as follows: PD-1 (CST, 86163S and 84651S; eBioscience, 12-9969-41), mouse immunoglobulin 1κ (IgG1κ) isotype control (eBioscience, 12-4714-81), p53 (Santa Cruz Biotechnology, sc-126; Leica Biosystems, P53-CM5P; CST, 2524S), p21 (Santa Cruz Biotechnology, sc-6246), vinculin (Sigma-Aldrich, V9131), normal IgG (Santa Cruz Biotechnology, sc-2025 and sc-2027), p300 (CST, 54062S), CBP (Santa Cruz Biotechnology, sc-7300-x), Flag (Sigma-Aldrich, A2220 and F7425), H3K18ac (Abcam, ab1191), H3K27ac (Abcam, ab4729), H4K16ac (Millipore, 07-329), HA (Sigma-Aldrich, A2095; Roche, 11867423001), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; Santa Cruz Biotechnology, sc-32233), HDAC1 (Santa Cruz Biotechnology, sc-81598), H3 (CST, 4499T; Abcam, ab1791), Ki-67 (CST, 9449S), p-AKT-S473 (CST, 4060S), p-mTOR-S2448 (CST, 2976S), Ac-p53-K120 (homemade), and Ac-p53-K164 (homemade).

Cao Z., Kon N., Liu Y., Xu W., Wen J., Yao H., Zhang M., Wu Z., Yan X., Zhu W.G., Gu W, & Wang D. (2021). An unexpected role for p53 in regulating cancer cell–intrinsic PD-1 by acetylation. Science Advances, 7(14), eabf4148.

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Signaling Pathway Analysis Protocol

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LOX (Novus Biologicals, NB100-2527), FoxF1 (Human Protein Atlas project, HPA003454 (mAb FoxF1 3454)), FAK-p^Y576 and FAK (Invitrogen), FAK-p^Y396 (Santa Cruz), α-tubulin (Sigma), Smad2-p^Ser465–467 (Calbiochem), Smad2/3 (Cell Signaling), p38-p^T180-Y182 and p38 (Cell signaling), HDAC-1 (Santa Cruz), p130Cas-p^Y249 and p130Cas (Cell signaling).

Nilsson G, & Kannius-Janson M. (2016). Forkhead Box F1 promotes breast cancer cell migration by upregulating lysyl oxidase and suppressing Smad2/3 signaling. BMC Cancer, 16, 142.

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Protein Expression Analysis of Treated Cells

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Untreated and treated cells were processed for protein extraction with lysis buffer 1× (Cell Signaling, Denver, CA, USA), supplemented with phosphatase and protease inhibitor (Sigma, Saint Louis, MO, USA). The whole cell lysate of each sample was subjected to SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare, Chicago, IL, USA). The membranes were saturated for 1 h at room temperature with 5% nonfat milk in TBS-Tween and incubated with the following primary antibodies: anti-CD44 (Abcam, Cambridge, UK), FASN (Gene Tex, Zeeland, MI, USA), caspase 3 cleaved (Cell Signaling, Denver, CA, USA), GAPDH (Santacruz, Dallas, TX, USA), HDAC1 (Santacruz, Dallas, TX, USA), and HDAC3 (Santacruz, Dallas, TX, USA) at concentrations recommended by the suppliers. Band identification was performed by exposing the membranes to the enhanced chemiluminescence (ECL) substrate, and the images were acquired using a ChemiDoc Molecular Imager (Biorad, Hercules, CA, USA). The procedures were performed according to the protocols provided by manufacturers.

Colapietro A., Mancini A., Vitale F., Martellucci S., Angelucci A., Llorens S., Mattei V., Gravina G.L., Alonso G.L, & Festuccia C. (2020). Crocetin Extracted from Saffron Shows Antitumor Effects in Models of Human Glioblastoma. International Journal of Molecular Sciences, 21(2), 423.

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