Glass slide

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany, United Kingdom

Glass slides are flat and smooth surfaces made of glass, commonly used in various laboratory applications. They provide a clear, transparent surface for holding and mounting samples for microscopic examination and analysis.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (10 mentions) Triton x 100, by Merck Group (8 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions) Glass coverslip, by Thermo Fisher Scientific (6 mentions) Bovine serum albumin (bsa), by Merck Group (5 mentions) Cryostat, by Leica (5 mentions) Sylgard 184, by Dow (5 mentions) Poly l lysine (pll), by Merck Group (4 mentions) Lsm 780, by Zeiss (4 mentions) Alexa fluor 488, by Thermo Fisher Scientific (3 mentions) Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (3 mentions) Formaldehyde, by Merck Group (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (3 mentions) Glycerol, by Merck Group (3 mentions) Oct compound, by Thermo Fisher Scientific (3 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (3 mentions) Paraformaldehyde (pfa), by Merck Group (3 mentions) Prolong antifade, by Thermo Fisher Scientific (3 mentions) Sp5 confocal microscope, by Leica (2 mentions)

Close spelling variants of this product

LabTek chamber glass slides Polysine®-coated glass slides Superfrost Ultra Plus glass slides Frosted glass slides Nunc Lab-Tec II 16-well glass chamber slides Two-chamber glass slides Lab-Tek four-well glass chamber slides Borosilicate glass slides LabTekII glass chamber slides 8-well chambered cover glass slides

176 protocols using glass slide

Immunofluorescence Staining of BxPC-3 Cells

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BxPC-3 cells, cat. no. CRL-1687 (ATCC, Manassas, VA)

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Cover Glasses, round, 18 mm diameter, cat. no. 12-548A (ThermoFisher Scientific, Waltham, MA).

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Sterile 12-well multi-well plates, cat. no. 712001 (Bioland Scientific, Paramount, CA)

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Forceps, fine tip, 5 in., cat. no. 3120019 (ThermoFisher Scientific, Waltham, MA).

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Glass slides, cat. no. 12550003 (ThermoFisher Scientific, Waltham, MA).

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Parafilm, cat. no.1337410 (ThermoFisher Scientific, Waltham, MA).

Baik M., French B., Chen Y.C., Byers J.T., Chen K.T., French S.W, & Díaz B. (2019). Identification of invadopodia by TKS5 staining in human cancer lines and patient tumor samples. MethodsX, 6, 718-726.

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Confocal Microscopy Imaging of Fixed Samples

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For microscopy experiments, the samples were fixed in 4% Formaldehyde (Merck) in PBS and mounted onto glass slides (Thermo Scientific) using Prolong Gold mounting medium with DAPI (Invitrogen). Z stacks of 5 images per cell were collected on a Leica SP5 confocal microscope equipped with HyD detectors, using a 63× magnification lens in combination with 2.5–4× digital zoom and represented as maximum z projections. Image processing and fluorescence intensity analysis was performed using ImageJ64 software, and colocalization was expressed in the form of Mander’s overlap coefficients calculated using JACoP. Pixel plot analyses were generated using Leica LASAF software.

Mulder M.P., Witting K., Berlin I., Pruneda J.N., Wu K.P., Chang J.G., Merkx R., Bialas J., Groettrup M., Vertegaal A.C., Schulman B.A., Komander D., Neefjes J., Oualid F.E, & Ovaa H. (2016). A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature chemical biology, 12(7), 523-530.

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Mitochondrial Localization of G0S2 in CD8+ T Cells

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CD8⁺ T cells transduced with MIGR1-G0S2-V5 were cytospun onto glass slides (Thermo Scientific, Waltham, Massachusetts, U.S.A.) and immediately fixed with 1% (vol/vol) paraformaldehyde. Fixed cells were permeabilized with 0.1% (vol/vol) Triton X-100. Slides were stained with mouse anti-V5 and a mouse-specific secondary antibody conjugated to Alexa Fluor 555 (LifeTechnologies, Grand Island, New York, U.S.A.). Mitochondrial localization was detected with rabbit anti-COX IV (Abcam, Cambridge, Massachusetts, U.S.A.) and a rabbit-specific secondary antibody conjugated to Alexa Fluor 488 (LifeTechnologies, Grand Island, New York, U.S.A.). The slides were mounted with a mounting solution containing 4’,6-diamidino-2-phenylindole (DAPI) (LifeTechnologies, Grand Island, New York, U.S.A.) to stain the nuclei and analyzed on an Eclipse 90i microscope using the NIS Elements imaging software (Nikon Instruments Inc., Melville, New York, U.S.A.).

Lee P.H., Yamada T., Park C.S., Shen Y., Puppi M, & Lacorazza H.D. (2015). G0S2 modulates homeostatic proliferation of naïve CD8+ T cells and inhibits oxidative phosphorylation in mitochondria. Immunology and cell biology, 93(7), 605-615.

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Quantifying DNA Damage via γH2AX

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For γH2AX immunofluorescence staining, 5x10⁴ cells were seeded on glass slides (Thermo Fisher Scientific) and incubated overnight before adding treatments (Doxorubicin, carboplatin, olaparib). Forty-eight hr later, cells were fixed with formalin 10% (Sigma) for 10 min at room temperature, washed with PBS 1 X and then permeabilized with 0.25% Triton-X100 (Sigma) in PBS 1 X for 15 min. Slides were incubated 1 hr with DAKO blocking solution (Agilent) and incubated overnight at 4 C with anti-phospo-H2AX (1:2000). Cells were then washed three times with PBS 1X-Tween 0.05% followed by incubation with the secondary antibody (1:800; donkey anti-mouse IgG Alexa Fluor 488; Thermo Fisher Scientific). The glass slides were stained with DAPI, washed, and mounted with Fluoromount aqueous mounting medium (Sigma). Stained sections were scanned (Leica) by the molecular pathology core of the CRCHUM and photographs were analyzed using Visomorph viewer software for automatic counting of γH2AX foci.

Allard D., Cousineau I., Ma E.H., Allard B., Bareche Y., Fleury H, & Stagg J. (2023). The CD73 immune checkpoint promotes tumor cell metabolic fitness. eLife, 12, e84508.

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Microfluidic Device Fabrication for Parallel Analysis

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The microfluidic devices were manufactured using standard microfabrication techniques. In brief, a single-layer photoresist design (SU-8; MicroChem, Newton, MA), with a 50-μm-thick layer was patterned on one silicon wafer via a photolithography masks and standard processing, according to the manufacturer’s protocols. The resulting patterned wafer was then used as a mold to produce polydimethylsiloxane (Thermo Fisher Scientific, Waltham, MA) devices, which were subsequently, irreversibly bonded to glass slides (1 in. × 3 in.; Thermo Fisher Scientific). The microfluidic design included four channels, each with their own inlet and outlet ports, and one common central imaging area (Supplementary Fig. 1,A and B, Supplemental Digital Content 1, http://links.lww.com/CCX/A66). This configuration allowed for the simultaneous imaging and analysis of multiple conditions (Supplementary Fig. 1C, Supplemental Digital Content 1, http://links.lww.com/CCX/A66). The chips were pretreated with a corona plasma gun (Elveflow, Paris, France) prior to sample loading to eliminate the need for fluid pumps.

Frydman G.H., Ellett F., Van Cott E.M., Hayden D., Majmudar M., Vanderburg C.R., Dalzell H., Padmanabhan D.L., Davis N., Jorgensen J., Toner M., Fox J.G, & Tompkins R.G. (2019). A New Test for the Detection of Direct Oral Anticoagulants (Rivaroxaban and Apixaban) in the Emergency Room Setting. Critical Care Explorations, 1(8), e0024.

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Histological Analysis of Mouse Skeletal Muscle

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TA muscles were dissected from mice, fixed in 4 % paraformaldehyde overnight followed by 70 % ethanol overnight, and then embedded in paraffin following standard protocols. Tissues were cut with a microtome in a cross-sectional orientation through the entire length of the muscle. Cross sections of 5 mm thickness were then mounted onto glass slides (Thermo Fisher Scientific, USA) and stained with Masson’s trichrome or Picrosirius red following standard protocols. The cross-sectional area was used as a measure of myofiber size, which was produced by semi-automated measurements on stitched whole-section images (Nikon).

Zhang R.H., Judson R.N., Liu D.Y., Kast J, & Rossi F.M. (2016). The lysine methyltransferase Ehmt2/G9a is dispensable for skeletal muscle development and regeneration. Skeletal Muscle, 6, 22.

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Mitochondrial Imaging with MitoTracker

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Cells (10⁵) were incubated with 200 nM MitoTracker Deep Red dye (Thermo-Fischer Scientific) for 30 min, and transferred to 0.01% poly-L-lysine (Sigma-Aldrich) coated glass slides (Thermo-Fisher Scientific). Next, cells were fixed 15 min in 4% formaldehyde then cold 100% methanol and mounted in ProLongTM Gold antifade medium with DAPI (Invitrogen). Images were acquired using a Zeiss LSM 800 microscope with Airyscan.

Larrue C., Mouche S., Lin S., Simonetta F., Scheidegger N.K., Poulain L., Birsen R., Sarry J.E., Stegmaier K, & Tamburini J. (2023). Mitochondrial fusion is a therapeutic vulnerability of acute myeloid leukemia. Leukemia, 37(4), 765-775.

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Immunohistochemical Analysis of Placental Tissues

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Placentas were fixed in 4% paraformaldehyde for 24–36 h following a standard protocol, then dehydrated and embedded in paraffin. Tissue sections (5-μm thickness) were mounted on glass slides (Thermo Scientific, Waltham, MA, USA). Rabbit anti-E-cadherin, anti-vimentin, and anti-β-cadherin polyclonal antibodies (1:100; Cell Signaling Technology) were used as primary antibodies, and anti-rabbit IgG (1:200; Sigma-Aldrich) used as a secondary antibody. Sections were mounted onto slides using Gel Mount Aqueous Mounting Medium (Sigma-Aldrich) and examined with an Olympus BX51 microscope.

Zuo Q., Huang S., Zou Y., Xu Y., Jiang Z., Zou S., Xu H, & Sun L. (2016). The Lnc RNA SPRY4-IT1 Modulates Trophoblast Cell Invasion and Migration by Affecting the Epithelial-Mesenchymal Transition. Scientific Reports, 6, 37183.

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Gelatin-Based Photocrosslinkable Hydrogels

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Gelatin (Type A, 300 bloom from porcine skin), methacrylate anhydride (MA), NaOH pellets, NaCl, 3-(trimethoxysilyl)-propyl methacrylate (TMSPMA), and ferritin and apoferritin (both from equine spleen) were purchased from Sigma-Aldrich (St. Louis, Missouri). Irgacure 2959 (2-hydroxy-1-(4-(hydroxyethoxy)-phenyl)-2-methyl-1-propanone), as a photoinitiator (PI), was acquired from Ciba Specialty Chemicals Incorporated (Basel, Switzerland). Dulbecco’s phosphate-buffered saline (DPBS), Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin–streptomycin were bought from Invitrogen (Waltham, Massachusetts). Dialysis membrane tubes, Slide-A-Lyzer Dialysis cassette, glass slides, and coverslips were purchased from Thermo Fisher Scientific (Waltham, Massachusetts). Alexa Fluor 488 Phalloidin, 4′,6-diamidino-2-phenylindole (DAPI), LIVE/DEAD kit, and fluoraldehyde o-phthaldialdehyde (OPA) were acquired from Thermo Fisher Scientific (Waltham, Massachusetts). Omnicure S2000 (EXFO Photonic Solutions Incorporation, Quebec City, Canada) was used for photo-cross-linking. The device for mechanical testing was the 5942 Single Column Tabletop Model testing system from Instron, an ITW company (Illinois Tool Works Incorporation, Glenview, Illinois).

Samanipour R., Wang T., Werb M., Hassannezhad H., Rangel J.M., Hoorfar M., Hasan A., Lee C.K, & Shin S.R. (2019). Ferritin Nanocage Conjugated Hybrid Hydrogel for Tissue Engineering and Drug Delivery Applications. ACS biomaterials science & engineering, 6(1), 277-287.

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Immunofluorescent Detection of Phospho-AMPK

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Cells were cultured for 2 days and fixed with 4% paraformaldehyde (PFA) for 10 min, permeabilised in 0.4% TX-100 in phosphate-buffered saline (PBS) for 10 min, blocked in 5% normal goat serum in PBS for 30 min and incubated with rabbit phospho-AMPK (1∶100, Cell Signaling Technology) antibody in blocking buffer overnight at 4°C, washed three-times with PBS, for 10 min and incubated with Alexa Fluor 488 conjugated secondary antibody (1∶1,000, Molecular Probes) for 2 hr, stained with Hoechst 33342 (1∶10,000, Invitrogen) for 15 min, washed three times with PBS for 10 min and mounted using fluorescent mounting medium (Dako) on glass slides (Thermo Scientific) for microscopy using an Olympus FV 1000 confocal microscope. Images were captured using identical exposure and gain settings. Negative controls without primary antibodies were performed which produced no staining.

Perera N.D., Sheean R.K., Scott J.W., Kemp B.E., Horne M.K, & Turner B.J. (2014). Mutant TDP-43 Deregulates AMPK Activation by PP2A in ALS Models. PLoS ONE, 9(3), e90449.

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