Anti cd73

Kidney capsule grafts or lung slides were fixed with 4% paraformaldehyde and embedded in OCT. Samples were sectioned at 6–12 μm sections. The following primary antibodies were used: anti-human CC10 (1/1,000, Santa Cruz, sc-365992), anti-human SPC (1/1,000, Santa Cruz, sc-7705), anti-human AQP5 (1/500, Santa Cruz, sc-9890), anti-human LGR6 (1/1,000, Santa Cruz, SC-48236), anti-human SDF-1 (1/500, Santa Cruz, sc-6193), anti-human CXCR4 (1/500, Santa Cruz, sc-9046), anti-GFP (1/1,000, Abcam, ab-13970), anti-RFP (1/1,000, Abcam, ab-62341), anti-CD73 (1/1,000, Abcam, ab-54217), anti-mouse Vimentin (1/1,000, BD Pharmigen, #550513), anti-human Nuclei antibody (1/1,000, Millipore, MAB1281). Sections were incubated in blocking buffer (PBS, 4% donkey serum, 1% Triton) for 1 h at room temperature. Primary antibodies were incubated overnight at 4 °C. Sections were rinsed three times in PBS and incubated with secondary antibodies diluted at 1:1,000 for 1 h at room temperature. Slides were mounted in Vectashield mounting media with DAPI (4′,6-diamidino-2-phenylindole).

Human abdominal subcutaneous adipose tissues were harvested during liposuction procedures after obtaining signed informed consent in the Department of Plastic Surgery, Affiliated Hospital of Zunyi Medical University, China. ADSC isolation and culture was performed as previously described.¹⁵ Briefly, human adipose tissue was minced and digested with 0.075% collagenase type I (Sigma) for 45 minutes at 37°C. After digestion, an equal volume of Dulbecco's modified Eagle's medium (DMEM; Gibco, Carlsbad, California) supplemented with 10% fetal bovine serum (FBS; Gibco) was added and the suspension was passed through a 200 μm mesh filter followed by centrifugation at 800g for 5 minutes). The stromal‐vascular fraction cell pellets were resuspended and cultured at 37°C in 5% CO₂ in DMEM supplemented with 10% FBS and 1% penicillin‐streptomycin (Gibco). ADSCs were subcultured at 80% confluence, and passage‐3 cells were used in the study procedures. ADSCs surface marker expression was assayed by flow cytometry. Suspensions of 1 × 10⁶ ADSCs were incubated with anti‐CD90, anti‐CD73, anti‐ CD105, anti‐CD34, anti‐CD11b, anti‐CD19, anti‐CD45, or anti‐HLA‐DR antibodies (1 mg/mL; Abcam) at room temperature for 30 minutes, washed with phosphate‐buffered saline (PBS), and analyzed with a MoFlo XDP flow cytometer (Beckman Coulter, Brea, California) and Kaluza software (Beckman Coulter).