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Mini opticon real time pcr machine

Manufactured by Bio-Rad
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The Mini Opticon Real Time PCR machine is a compact and versatile thermal cycler designed for real-time PCR applications. It features a four-color optical system and can accommodate up to 48 samples in a single run. The instrument is capable of performing precise temperature control and fluorescence detection, making it suitable for a variety of real-time PCR experiments.

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Lab products found in correlation

Sypro orange, by Merck Group (1 mentions) Galnac, by Merck Group (1 mentions) Sypro orange, by Thermo Fisher Scientific (1 mentions) Opticon monitor 3, by Bio-Rad (1 mentions) Rneasy kit, by Qiagen (1 mentions) Superscript first strand cdna synthesis system, by Thermo Fisher Scientific (1 mentions) Power sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) Itaq sybr green, by Bio-Rad (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Cfx connect real time pcr machine, by Bio-Rad (1 mentions)

Close spelling variants of this product

PCR machine (73 citations) Opticon real-time PCR machine (7 citations)

4 protocols using mini opticon real time pcr machine

1

Thermal Shift Assay for Glycan Binding

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Thermal shift assays were performed using a Mini Opticon Real Time PCR machine (BioRad). 0.6 mg/mL protein in PBS was mixed with SYPRO Orange (Sigma-Aldrich, Merck, #S5692) and glycan ligand (10 mM GalNAc; Carbosynth, #MA04390; 10 mM GlcNAc, Carbosynth, #MA00834; 10 mM blood group H type-2 tetrasaccharide; Elicityl, GLY032-2) in a total reaction volume of 25 µL. The temperature was raised by 1 °C/min from 25 to 100 °C, and fluorescence readings were taken at each step.
Lundstrøm J., Gillon E., Chazalet V., Kerekes N., Di Maio A., Feizi T., Liu Y., Varrot A, & Bojar D. (2024). Elucidating the glycan-binding specificity and structure of Cucumis melo agglutinin, a new R-type lectin. Beilstein Journal of Organic Chemistry, 20, 306-320.
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2

Thermal Shift Assay for Protein-Ligand Interactions

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Thermal shift assays were performed using a Mini Opticon, Real Time PCR machine (Bio-Rad Laboratories) with 0.5 mg ml-1 of protein diluted in 100 mM NaCl, 20 mM Tris pH 7.5, 100 μM CaCl2, with or without oligosaccharide in a total reaction volume of 25 μl. SYPRO Orange (Molecular Probes, CA) was used as a fluorescent probe detected at 530 nm. The temperature was raised using 1°C/minute steps from 25°C to 100°C and fluorescence readings were taken at each interval. A positive ΔTm value indicates that the ligand stabilizes the protein from denaturation, and therefore binds to the protein. A minimum of two independent measurements were performed for each condition
Audfray A., Beldjoudi M., Breiman A., Hurbin A., Boos I., Unverzagt C., Bouras M., Lantuejoul S., Coll J.L., Varrot A., Le Pendu J., Busser B, & Imberty A. (2015). A Recombinant Fungal Lectin for Labeling Truncated Glycans on Human Cancer Cells. PLoS ONE, 10(6), e0128190.
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3

Quantification of cAMP Modulators in Astrocytes

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RNA was extracted from WT and Nf1-/- astrocytes using the Qiagen RNeasy kit (Qiagen, Valenica, CA). cDNA was generated using the Superscript first-strand cDNA synthesis system (Invitrogen). Real-time quantitative PCR reactions were performed using Power Sybr Green PCR master mix (Applied Biosystems (Carlsbad, CA)) using primers as indicated in Supplemental Table 3. Triplicate measures were made for each sample and corresponding GAPDH control. PCR and data collection were done using the BioRad MiniOpticon Real Time PCR machine and Opticon Monitor 3 Software from BioRad (Hercules, CA). Relative transcript copy number was calculated using the delta-delta-C(t) method. The relative expression values of cAMP modulators in cells derived from female Nf1-/- astrocytes were normalized to those from male expression levels (n = 3-5 separate litters/genotype).
Warrington N.M., Sun T., Luo J., McKinstry R.C., Parkin P.C., Ganzhorn S., Spoljaric D., Albers A.C., Merkelson A., Stewart D.R., Stevenson D.A., Viskochil D., Druley T.E., Forys J.T., Reilly K.M., Fisher M.J., Tabori U., Allen J.C., Schiffman J.D., Gutmann D.H, & Rubin J.B. (2014). The cyclic AMP pathway is a sex-specific modifier of glioma risk in type 1 neurofibromatosis patients. Cancer research, 75(1), 16-21.
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4

Real-Time qPCR for Gene Expression Analysis

Cited 1 time
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RNA was harvested from tissue or dissociated cells using TRIzol (Invitrogen), and cDNA was prepared for PCR using standard methods (22 (link)). Real-time qPCR was performed using gene-specific primers (Supplementary Table S1) and either Power Sybr Green Master Mix Reagent (Life Technologies) or iTaq Sybr Green (Bio-Rad), with either a MiniOpticon Real-Time PCR machine or CFX Connect Real-Time PCR machine (Bio-Rad). Data were analyzed according to the ΜCt method (2−ΔΔt), where ΔCt is the difference between the gene of interest and GAPDH control Ct value, and data were normalized to the average of all vehicle-treated samples as in refs. 21 (link), 22 (link).
Ward S.A., Warrington N.M., Taylor S., Kfoury N., Luo J, & Rubin J.B. (2016). Reprogramming Medulloblastoma-Propagating Cells by a Combined Antagonism of Sonic Hedgehog and CXCR4. Cancer research, 77(6), 1416-1426.
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