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Pluronic f127

Manufactured by BASF
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Sourced in Germany, United States, France, India, China, Japan

Pluronic F127 is a non-ionic triblock copolymer composed of polyethylene oxide (PEO) and polypropylene oxide (PPO) blocks. It has the chemical formula HO(C2H4O)a(C3H6O)b(C2H4O)aH, where 'a' and 'b' represent the number of repeating units. Pluronic F127 is a versatile material used in various applications, including pharmaceuticals, cosmetics, and biotechnology.

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Lab products found in correlation

Cremophor el, by BASF (5 mentions) Pluronic f68, by BASF (5 mentions) Polyvinyl alcohol (pva), by Merck Group (4 mentions) Methanol, by Merck Group (4 mentions) Transcutol p, by Gattefosse (3 mentions) Labrasol, by Gattefosse (3 mentions) Capryol 90, by Gattefosse (3 mentions) Pluronic p123, by BASF (3 mentions) Milli q water purification system, by Merck Group (3 mentions) Ethanol, by Merck Group (3 mentions) Curcumin, by Merck Group (3 mentions) Propylene glycol, by Merck Group (3 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions) Egg lecithin e80, by Lipoid (2 mentions) L glutamine, by Merck Group (2 mentions) Dulbecco s modified eagle s medium, by Merck Group (2 mentions) Soluplus, by BASF (2 mentions) Fluo 3, by Thermo Fisher Scientific (2 mentions) Ensure h, by Abbott (2 mentions) Milli q system, by Merck Group (2 mentions)

Close spelling variants of this product

Pluronic F127 (F127) (5 citations) Pluronic F127 (Poloxamer 407) (4 citations) Pluronic® F127 Prill (2 citations) F127 Pluronic Acid (2 citations)

87 protocols using pluronic f127

1

Formulation and Characterization of Lipid-based Nanocarriers

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Polyoxyethylene castor oil (Cremophor®EL) was purchased from BASF SE (Ludwigshafen, Germany); highly purified diethylene glycol monoethyl ether (Transcutol®P) was obtained from Gattefossé (Saint-Priest Cedex, France); CRT was purchased from Sigma Aldrich (St. Louis, MO). Lipoid GmBH (Köln, Germany) provided free Phospholipon 90G samples. From S.D. Fine Chemicals Ltd. (Mumbai, India), Tween 20, Tween 80, Span 20, and Span 80 were purchased. Absolute ethanol was purchased from Bengal Chemicals Ltd. (Kolkata, India). Pluronic®F-127 was procured from BASF Corporation (Florham Park, NJ). In this work, all other compounds were of analytical quality, and double distilled water was used.
Chen L., Alrobaian M., Afzal O., Kazmi I., Panda S.K., Alfawaz Altamimi A.S., Al-Abbasi F.A., Almalki W.H., Katouah H.A., Singh T., Soni K., Hafeez A., Beg S., Kumar V, & Rahman M. (2021). Crotamiton-loaded tea tree oil containing phospholipid-based microemulsion hydrogel for scabies treatment: in vitro, in vivo evaluation, and dermatokinetic studies. Drug Delivery, 28(1), 1972-1981.
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2

Chitosan-Based Nanoparticle Formulation

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Chitosan (MW 100 kDa; approximately 90% deacetylated) was obtained from Golden-Shell Biochemical Co. Ltd. (Zhejiang, People’s Republic of China). Egg lecithin (Lipoid E80) was obtained from Lipoid GmbH (Ludwigshafen, Germany). Pluronic F127 was a kind gift from BASF (Ludwigshafen, Germany). HPMC and hydroxyethyl cellulose (HEC) were purchased from Colorcon Co. Ltd. (Shanghai, People’s Republic of China) and Ashland-Aqualon Inc. (Wilmington, DE, USA), respectively. Negative control siRNA and Cy5-labeled siRNA (Cy5-siRNA) were purchased from Shanghai GenePharma Co. Ltd. (Shanghai, People’s Republic of China) and Biomics Biotechnologies Co. Ltd. (Jiangsu, People’s Republic of China), respectively. All other chemicals and solvents were of analytical reagent grade.
Wu N., Zhang X., Li F., Zhang T., Gan Y, & Li J. (2015). Spray-dried powders enhance vaginal siRNA delivery by potentially modulating the mucus molecular sieve structure. International Journal of Nanomedicine, 10, 5383-5396.
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3

Budesonide-Loaded Polymeric Nanoparticles

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Budesonide, methylcellulose (Methocel 90HG), and ethanol (96%) were purchased from Sigma Aldrich; poly(methacrylic acid-co-methyl methacrylate) 1:1 (Eudragit® L100) from Evonik Röhm GmbH (Darmstadt, Germany); polyvinyl alcohol (PVA 22000) from Fluka Chemie AG (Germany); and Pluronic F127 from BASF (Ludwigshafen, Germany). Distilled water was prepared in the laboratory. For HPLC analysis, acetonitrile and methanol HPLC grades were used from Fisher Chemical (Thermo Fisher Scientific Inc., Waltham, MA, USA). Dulbecco’s Modified Eagle’s Medium, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), fetal bovine serum, and L-glutamine were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). The Human immortalized keratinocyte cell line HaCaT (300493) was acquired from the CLS Cell Lines Service GmbH (CLS, Eppelheim, Germany).
Slavkova M., Lazov C., Spassova I., Kovacheva D., Tibi I.P., Stefanova D., Tzankova V., Petrov P.D, & Yoncheva K. (2024). Formulation of Budesonide-Loaded Polymeric Nanoparticles into Hydrogels for Local Therapy of Atopic Dermatitis. Gels, 10(1), 79.
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4

Levofloxacin-Loaded PLLA Microfiber Sutures

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Levofloxacin microfiber sutures were manufactured using the wet electrospinning setup depicted in Figure 1A and described by Zhang and coworkers.37 (link) Briefly, PLLA (221 kDa; Corbion, Amsterdam, Netherlands) at 86% to 89% (wt/wt) was mixed with Levofloxacin (Sigma Aldrich, St. Louis, MO) at 10 wt% and either PEG (35 kDa; Sigma Aldrich) or Pluronic F127 (BASF, Florham Park, NJ) between 1 and 4 wt% and dissolved in chloroform (Sigma Aldrich) at room temperature for 24 hours. Levofloxacin concentration was held constant and PLLA concentration in chloroform was maintained at 15 wt% in all formulations. Sutures were produced by wet electrospinning the polymer/drug solution in a setup consisting of a high voltage power supply (Gamma High Voltage Research, Ormond Beach, FL), syringe pump (Fisher Scientific, Waltham, MA), and rotating metal collector with hexane (Sigma Aldrich) as the lending solvent. The polymer solution was ejected through a blunted 18-G needle (Fisher Scientific) at 13 mL/h with 4.7 kV of applied voltage 5 cm away from the collector rotating at 40 rpm. Fibers were then collected and desiccated for 2 days prior to storage at −20°C.
Kashiwabuchi F., Parikh K.S., Omiadze R., Zhang S., Luo L., Patel H.V., Xu Q., Ensign L.M., Mao H.Q., Hanes J, & McDonnell P.J. (2017). Development of Absorbable, Antibiotic-Eluting Sutures for Ophthalmic Surgery. Translational Vision Science & Technology, 6(1), 1.
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5

Carfilzomib Drug Formulation Protocol

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Carfilzomib (CFZ) was purchased from Shenzhen Chemical Co. Ltd. (Shanghai, China). Pluronic F127 was obtained from BASF (Florham Park, NJ, USA). Sulfobutylether-β-cyclodextrin (Captisol®) was a gift from Captisol, Inc. (San Diego, CA, USA). Human serum albumin (HSA; ≥96% purity assessed by agarose gel electrophoresis) was purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were purchased from Fisher Scientific (Waltham, MA, USA) and were used as received.
Gadalla H.H., Lee S., Kim H., Armstrong A.T., Fathalla D., Habib F., Jeong H., Lee W, & Yeo Y. (2022). Size optimization of carfilzomib nanocrystals for systemic delivery to solid tumors. Journal of controlled release : official journal of the Controlled Release Society, 352, 637-651.
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6

Docetaxel Nanoparticle Synthesis and Characterization

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Docetaxel (DTX) was purchased from LC Laboratories (Woburn, MA, USA). Pluronic F127 (F127) was a gift from BASF (New York, NY, USA). Human serum albumin (≥ 96% agarose gel electrophoresis) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Reagents for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were purchased from Bio-Rad (Hercules, CA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Hoechst 33342 were purchased from Invitrogen (Eugene, OR, USA). Antibodies against human SPARC (hSPARC) and mouse Sparc (mSparc) were from R&D system (Minneapolis, MN, USA). The antibody against β-actin was from Cell Signaling (Danvers, MA, USA). All other reagents were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Gad S.F., Park J., Park J.E., Fetih G.N., Tous S.S., Lee W, & Yeo Y. (2018). Enhancing docetaxel delivery to multidrug-resistant cancer cells with albumin-coated nanocrystals. Molecular pharmaceutics.
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7

Doxorubicin Delivery System Development

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Doxorubicin hydrochloride (99.9%) was purchased from LKM Laboratories (Argentina). Pluronic ® F127 (F127, MW ∼12.6 kDa, 70 wt % PEO), Tetronic ® 1107 (T1107, MW ∼15.0 kDa, 70 wt% PEO) and Soluplus ® (MW ∼ 120 KDa) were a gift of BASF (Argentina). TPGS (MW ∼1513 g/mol) was purchased from Eastman Chemical Company (USA), sodium deoxycholate (NaDC) was purchased from Riedel-de Häen ® (Germany). Doxil ® was purchased from Raffo Laboratories (Argentina). Tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H tetrazolium], inner salt (MTS) and phenazinemethosulfate (PMS) were purchased from Promega Corporation (USA). All solvents were analytical or high performance liquid chromatography (HPLC) grade and were used following the manufacturer's instructions.
Cagel M., Bernabeu E., Gonzalez L., Lagomarsino E., Zubillaga M., Moretton M.A, & Chiappetta D.A. (2017). Mixed micelles for encapsulation of doxorubicin with enhanced in vitro cytotoxicity on breast and ovarian cancer cell lines versus Doxil®. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 95.
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8

Measuring Cardiomyocyte Calcium Transients

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To measure cellular calcium transients, both freshly isolated cardiomyocytes and primarily cultured cardiomyocytes were incubated with 5 µM Fluo‐3 (Invitrogen, Grand Island, NY, USA) and 0.01% Pluronic F127 (BASF, Florham Park, NJ, USA) in Tyrode's solution for 35 min.[29 (link)
] To measure intracellular Ca2+ transients, cardiomyocytes were electrically paced at 1 Hz. Images were obtained with an Olympus camera. The amplitude of the intracellular Ca2+ transient was calculated as the difference between peak and diastolic calcium levels according to the equation (F‐F0)/F0 after subtraction of background fluorescence. The calcium transient decay phase time constant (τd) was determined using exponential curve fitting. The experiments were performed at room temperature. Measurements were taken from more than 20 myocytes from three or more animals from each group.
Li C., Zhang Y., Shen J., Bao H., Zhao Y., Li D., Li S., Liu Y., Yang J., Zhou Z., Gao K., Zhao L., Pei Y., Lu Y., Pan Z, & Cai B. (2024). Cfp1 Controls Cardiomyocyte Maturation by Modifying Histone H3K4me3 of Structural, Metabolic, and Contractile Related Genes. Advanced Science, 11(11), 2305992.
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9

Dietary Fat-Induced Triglyceride Absorption

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Overnight‐fasted C57BL/6J mice underwent an MTT in the morning. Vehicle, CpdA (3, 10 mg·kg−1), or Cpd B (3, 10 mg·kg−1) was administered by gavage. The compound was suspended in 0.5% methylcellulose. Six‐and‐a‐half or 16.5 h after gavage, pluronic F‐127 (500 mg·kg−1, BASF, Ludwigshafen, Germany) was intraperitoneally injected to inhibit plasma triglyceride (TG) hydrolysis by lipoprotein lipase. Thirty minutes after injection, liquid meal (10 mL·kg−1) comprising an admixture of corn oil and Ensure‐H (3 : 17 v/v) (Abbott Japan Co., Ltd., Tokyo, Japan) was orally loaded. Blood samples were collected preload (defined as 0 h) and 2 and 4 h after fat load, and plasma TG levels were measured. The area under the curve (AUC) of chylomicron TG (CM/TG), which is synthesized from dietary fat in the small intestine, was calculated by subtracting the plasma TG levels of the meal‐unloaded group from that of the meal‐loaded group.
Mochida T., Take K., Maki T., Nakakariya M., Adachi R., Sato K., Kitazaki T, & Takekawa S. (2020). Inhibition of MGAT2 modulates fat‐induced gut peptide release and fat intake in normal mice and ameliorates obesity and diabetes in ob/ob mice fed on a high‐fat diet. FEBS Open Bio, 10(3), 316-326.
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10

Punica granatum Seed Oil Nanoformulation

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Punica granatum seed oil hydroxyphenethyl esters (PHE), commercialized as Delipidol®, were kindly provided by BioEurope (Solabia group, Anet, France). Pluronic® F127 was kindly provided by BASF (Levallois-Perret, France). Polyvinyl alcohol (Mw: 31,000–50,000 g/mol, 87–89% hydrolysed) (PVA), Vitamin E (VitE) and dimethyl carbonate (DMC) were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Glycerol was purchased from Cooper® (Melun, France) and SepicideTM HB from SEPPIC® (La garenne-Colombes, France). SepicideTM HB is a mixture of phenoxyethanol, methylparaben, ethylparaben, propylparaben and butylparaben, henceforth referred as AMA (for AntiMicrobial Agent) in the text. Ultrapure water was obtained with a MilliQ system (Millipore, Molsheim, France).
Van Gheluwe L., Munnier E., Kichou H., Kemel K., Mahut F., Vayer M., Sinturel C., Byrne H.J., Yvergnaux F., Chourpa I, & Bonnier F. (2021). Confocal Raman Spectroscopic Imaging for Evaluation of Distribution of Nano-Formulated Hydrophobic Active Cosmetic Ingredients in Hydrophilic Films. Molecules, 26(24), 7440.
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