Pyridyl disulfide ethyl methacrylate

Poly(ethylene glycol) methyl ether (4-cyano-4-pentanoate dodecyl trithiocarbonate) (PEG-CTA), 2-diisopropylaminoethyl methacrylate (DIPAMA), and pyridyl disulfide ethyl methacrylate (PDSEMA) were purchased from Sigma (Saint Louis, MO, USA). Fetal bovine serum (FBS) was purchased from R&D Systems (Minneapolis, MN, USA). Anti-mouse CTLA-4 (clone 9H10) and PD-1 (clone 29F.1A12) antibodies were purchased from Bio X cell. All other chemicals were purchased from Sigma and used as received.

RAFT CTA 4-cyanopentanoic acid dithio-benzoate (CPADB), N,N′-Azobisisobutyronitrile (AIBN), anhydrous N,N’-dimethylacetamide (DMAc, HPLC, 99.9%), methanol (MeOH, HPLC, 99.9%), and methylene chloride (DCM, HPLC, 99.9%) were purchased from Sigma-Aldrich and used without further purification. Pyridyl disulfide ethyl methacrylate (PDSEMA) was synthesized as described previously.[23 (link)] 2-(Dimethylamino)ethyl methacrylate (DMAEMA) and oligo(ethylene glycol) monomethyl ether methacrylate (OEGMA, Mn = 300 and pendent EO units DP 4~5), and 2-Diisopropylaminoethyl methacrylate (DIPAMA) were purchased from Sigma-Aldrich and were purified by passing through a column filled with basic alumina to remove the inhibitor prior to polymerization. Nile Red was purchased from ACROS Organics. Endotoxin-free plasmid pCMV-Luc™ (ProMega) and pmaxGFP™ (Lonza) were purified with the Qiagen Plasmid Giga kit (Qiagen) according to the manufacturer’s protocol.