Annexin 5 pe 7 add apoptosis detection kit

Manufactured by BD

Sourced in United States

The Annexin V-PE/7-ADD Apoptosis Detection Kit is a laboratory reagent used to detect and quantify apoptosis, a process of programmed cell death. The kit utilizes fluorescently-labeled Annexin V and 7-ADD dye to identify cells undergoing early and late stages of apoptosis, respectively.

Automatically generated - may contain errors

Lab products found in correlation

Cck 8 solution, by Dojindo Laboratories (4 mentions) Edu staining proliferation kit, by RiboBio (4 mentions) Ow cytometer, by BD (2 mentions) Modfit lt version 5.0 analysis software, by Verity Software House (1 mentions) Cell counting kit 8 cck 8 assay, by Dojindo Laboratories (1 mentions) Varioskan flash multimode reader, by Thermo Fisher Scientific (1 mentions) Propidium iodide, by Merck Group (1 mentions) Facscan, by BD (1 mentions) Expo32 adc software, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Annexin V-PE/7- ADD kit PE Annexin V Apoptosis Detection Annexin V Apoptosis Detection Kit Annexin V Apoptosis Kit Annexin V detection kit 7-ADD Annexin V-PE Apoptosis detection kit Annexin V kit Annexin V

8 protocols using annexin 5 pe 7 add apoptosis detection kit

Tofacitinib Modulates Jurkat Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 5×10³ ITK-SYK⁺ or ITK-SYK⁻ Jurkat cells per well were seeded in 96-well plate with RPMI-1640 medium containing DMSO, or 0.5, 2.5 or 5 µM tofacitinib for 6, 18, 24 and 48 h at 37°C. DMSO exposure at a concentration of 0.1% in controls had no effect on the viability of examined cells lines, therefore 0.1% DMSO was used to treated the cells for 6, 18, 24 and 48 h at 37°C; these cells were used as the control cells. Cell viability was evaluated using a Cell Counting Kit-8 assay (CCK-8; Dojindo Molecular Technologies, Inc.) according to the manufacturer's protocol. Cells were measured at 450 nm using a Varioskan Flash multimode reader (Thermo Fisher Scientific, Inc.).
The apoptotic rate of transduced cells that were treated with tofacitinib was detected using an Annexin V-PE/7-ADD Apoptosis Detection kit (BD Pharmingen™). Separately, the transduced and tofacitinib-treated cells (1×10⁶) were stained with propidium iodide (Sigma-Aldrich; Merck KGaA) at room temperature in the dark for 30 min. The cells were analysed using a flow cytometer using ModFit LT version 5.0 analysis software (Verity Software House, Inc.). Experiments were performed in triplicate.

Zhang L.L., Pan H.X., Wang Y.X., Guo T, & Liu L. (2019). Genome profiling revealed the activation of IL2RG/JAK3/STAT5 in peripheral T-cell lymphoma expressing the ITK-SYK fusion gene. International Journal of Oncology, 55(5), 1077-1089.

+ Open protocol

+ Expand

Evaluating HCC Cell Viability and Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The CCK-8 assay was used to assess cell viability. In brief, 1500 HCC cells were seeded into the 96-well plate. After 24, 48 and 72 h, the medium was added with 10 μL CCK-8 solution (Dojindo, Kumamoto, Japan) for 2.5 h, followed by detection of the absorption value using a microplate spectrophotometer. Besides, the DNA synthesis rate was assessed by using EdU Staining Proliferation Kit provided by RiboBio based on manufacturer’s protocol. The positive cells were observed and photographed using confocal laser microscope system. For detecting cell apoptosis, the Annexin V-PE/7-ADD Apoptosis Detection Kit (BD Biosciences, CA, USA) was used, in which HCC cells were incubated with 5 μL Annexin V-PE and 5 μL 7-ADD in 500 μL binding buffer, followed by analyzing apoptotic cells using a flow cytometer (BD Biosciences).

Sun S., Gao J., Zhou S., Li Y., Wang Y., Jin L., Li J., Liu B., Zhang B., Han S., Ding H, & Li X. (2020). A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling. Journal of Experimental & Clinical Cancer Research : CR, 39, 252.

+ Open protocol

+ Expand

Apoptosis Quantification in PASMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The apoptotic ratio of PASMCs cells was also determined using an annexin V-PE/7-ADD apoptosis detection kit (BD Bioscience, San Diego, CA, USA). After different treatments, 1 × 10⁵ cells were harvested, washed with PBS, and stained with annexin V-PE and 7-ADD according to the manufacturer’s instructions. Up to 1 × 10⁴ cells per sample were recorded using a Beckman CytoFLEX flow cytometer and analyzed with FlowJo software. Annexin V-PE-positive and 7-ADD-negative cells were considered to be apoptotic cells.

Huang S., Yue Y., Feng K., Huang X., Li H., Hou J., Yang S., Huang S., Liang M., Chen G, & Wu Z. (2020). Conditioned medium from M2b macrophages modulates the proliferation, migration, and apoptosis of pulmonary artery smooth muscle cells by deregulating the PI3K/Akt/FoxO3a pathway. PeerJ, 8, e9110.

+ Open protocol

+ Expand

Apoptosis Analysis in Leukemia Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The NALM-6 and Jurkat cells (1 × 10⁶ cells/well) were incubated with DFO alone or in combination with Dex for 48 h. After the cells were collected and washed and resuspended with cold PBS, an annexin V-PE/7-ADD apoptosis detection kit (BD Pharmingen™, CA, USA) was employed to detect the apoptosis in ALL cells. Eventually, total cell apoptosis was assessed using a Becton–Dickinson FACScan (BD Corporation, USA). Data were analyzed using the E Cell Quest software version 2.0 (BD Corporation, USA).

You H., Wang D., Wei L., Chen J., Li H, & Liu Y. (2022). Deferoxamine Inhibits Acute Lymphoblastic Leukemia Progression through Repression of ROS/HIF-1α, Wnt/β-Catenin, and p38MAPK/ERK Pathways. Journal of Oncology, 2022, 8281267.

+ Open protocol

+ Expand

Apoptosis Detection in SH-SY5Y Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Detection of apoptosis in SH-SY5Y cells was conducted using Annexin V-PE/7-ADD apoptosis detection kit (BD Biosciences, USA) by flow cytometry. The SH-SY5Y cells with the indicated treatment were collected and stained with 10 μl Annexin V-PE and 5 μl 7-ADD in 200 μl binding buffer for 20 min at room temperature in the dark. Eventually, total cell apoptosis was determined by a Beckman Coulter flow cytometer (Beckman USA) and the data were calculated with EXPO32 ADC software (Beckman).

Zhang L., Liu C., Huang C., Xu X, & Teng J. (2020). miR-155 Knockdown Protects against Cerebral Ischemia and Reperfusion Injury by Targeting MafB. BioMed Research International, 2020, 6458204.

+ Open protocol

+ Expand

Cell Viability, Proliferation, and Apoptosis Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

The CCK-8 assay was used to assess cell viability. In brief, 1500 HCC cells were seeded into the 96-well plate. After 24, 48 and 72h, the medium was added with 10μL CCK-8 solution (Dojindo, Kumamoto, Japan) for 2.5h, followed by detection of the absorption value using a microplate spectrophotometer. Besides, the DNA synthesis rate was assessed by using EdU Staining Proliferation Kit provided by RiboBio based on manufacturer's protocol. The positive cells were observed and photographed using confocal laser microscope system. For detecting cell apoptosis, the Annexin V-PE/7-ADD Apoptosis Detection Kit (BD Biosciences, CA, USA) was used, in which HCC cells were incubated with 5μL Annexin V-PE and 5μL 7-ADD in 500μL binding buffer, followed by analyzing apoptotic cells using a ow cytometer (BD Biosciences).

Sun S., Gao J., Zhou S., Li Y., Wang Y., Li J., Li J., Liu B., Zhang B., Han S., Ding H., & Li X. (2020). A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling.

+ Open protocol

+ Expand

Evaluating Cell Viability, Proliferation, and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The CCK-8 assay was used to assess cell viability. In brief, 1500 HCC cells were seeded into the 96-well plate. After 24, 48 and 72 h, the medium was added with 10µL CCK-8 solution (Dojindo, Kumamoto, Japan) for 2.5 h, followed by detection of the absorption value using a microplate spectrophotometer.
Besides, the DNA synthesis rate was assessed by using EdU Staining Proliferation Kit provided by RiboBio based on manufacturer's protocol. The positive cells were observed and photographed using confocal laser microscope system. For detecting cell apoptosis, the Annexin V-PE/7-ADD Apoptosis Detection Kit (BD Biosciences, CA, USA) was used, in which HCC cells were incubated with 5µL Annexin V-PE and 5µL 7-ADD in 500µL binding buffer, followed by analyzing apoptotic cells using a ow cytometer (BD Biosciences).

Sun S., Gao J., Zhou S., Li Y., Wang Y., Li J., Li J., Liu B., Zhang B., Han S., Ding H., & Li X. (2020). A Novel Circular RNA circ-LRIG3 Facilitates the Malignant Progression of Hepatocellular Carcinoma by Modulating the EZH2/STAT3 Signaling.

+ Open protocol

+ Expand

Assessing Hepatocellular Carcinoma Cell Viability

Check if the same lab product or an alternative is used in the 5 most similar protocols

The CCK-8 assay was used to assess cell viability. In brief, 1500 HCC cells were seeded into the 96-well plate. After 24, 48 and 72h, the medium was added with 10μL CCK-8 solution (Dojindo, Kumamoto, Japan) for 2.5h, followed by detection of the absorption value using a microplate spectrophotometer. Besides, the DNA synthesis rate was assessed by using EdU Staining Proliferation Kit provided by RiboBio based on manufacturer's protocol. The positive cells were observed and photographed using confocal laser microscope system. For detecting cell apoptosis, the Annexin V-PE/7-ADD Apoptosis Detection Kit (BD Biosciences, CA, USA) was used, in which HCC cells were incubated with 5μL Annexin V-PE and 5μL 7-ADD in 500μL binding buffer, followed by analyzing apoptotic cells using a flow cytometer (BD Biosciences).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!