Log In Sign up
The largest database of trusted experimental protocols

Chamber polystyrene vessels

Manufactured by BD
Visit Supplier
Sourced in United States

The Chamber Polystyrene Vessels are laboratory equipment designed to provide a controlled environment for various applications. They are made of polystyrene material and serve as a contained space for experimentation, sample preparation, or storage.

Automatically generated - may contain errors

Lab products found in correlation

Cultrex 3 d culture matrix reduced growth factor basement membrane extract, by R&D Systems (3 mentions) Anti e cadherin, by Cell Signaling Technology (2 mentions) Vimentin, by Santa Cruz Biotechnology (1 mentions) Fluorescence microscope, by Zeiss (1 mentions) Normal goat serum (ngs), by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Eight-chamber polystyrene vessel tissue culture-treated glass slides Poly-D-lysine-coated 8 chamber polystyrene vessel tissue culture treated glass slide

4 protocols using chamber polystyrene vessels

1

3D Culture and Immunofluorescence of MCF10A Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Seventy microliters of Cultrex 3-D Culture Matrix reduced growth factor basement membrane extract (R&D Systems) were added to triplicate wells of eight Chamber Polystyrene Vessels (BD Falcon). The vessels were placed in a 37°C 5% CO2 incubator for 30 min to allow the matrix to solidify. Cells (4000 per well) stably expressing vector control plasmid or B7-H3 overexpression plasmid were then seeded and allowed to grow for 8 days. The media was replaced with fresh growth media after 4 days. Acini ofMCF10A-Vector and MCF-10A-B7H3 were further fixed using a 1:1 mixture of methanol:acetone at −20°C for 10–12min. Acini were blocked with 2% FBS in PBS for 2 hours, then washed with PBS for 2 times. Acini were stain with anti-E-cadherin(Cell signaling) at 4°C overninght, and washed with PBS for 3 times. The Acini were stained with rhodamine-conjugated secondary antibody for 1–2 hr at room temperature in the dark, and the washed with PBS for 5 times. Slides were mounted with Prolong® Gold Antifade Reagent with DAPI.
Liu Z., Zhang W., Phillips J.B., Arora R., McClellan S., Li J., Kim J.H., Sobol R.W, & Tan M. (2018). Immunoregulatory Protein B7-H3 Regulates Cancer Stem Cell Enrichment and Drug Resistance through MVP-mediated MEK Activation. Oncogene, 38(1), 88-102.
+ Open protocol
+ Expand
2

3D Culture of MDA-MB-231 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Seventy microliters of Cultrex 3-D Culture Matrix reduced growth factor basement membrane extract (R&D Systems) were added to triplicate wells of eight Chamber Polystyrene Vessels (BD Falcon). The vessels were placed in a 37° 5% CO2 incubator for 30 min to allow the matrix to solidify. MDA-MB-231 cells (4000 per well) stably expressing vector control plasmid or mir-4728 overexpression plasmid were then seeded and allowed to grow for 8 days. The media was replaced with fresh growth media after 4 days.
Schmitt D.C., Madeira da Silva L., Zhang W., Liu Z., Arora R., Lim S., Schuler A.M., McClellan S., Andrews J.F., Kahn A.G., Zhou M., Ahn E.Y, & Tan M. (2015). ErbB2-intronic MicroRNA-4728: a novel tumor suppressor and antagonist of oncogenic MAPK signaling. Cell Death & Disease, 6(5), e1742-.
+ Open protocol
+ Expand
3

3D Culture and Immunofluorescence of MCF10A Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Seventy microliters of Cultrex 3-D Culture Matrix reduced growth factor basement membrane extract (R&D Systems) were added to triplicate wells of eight Chamber Polystyrene Vessels (BD Falcon). The vessels were placed in a 37°C 5% CO2 incubator for 30 min to allow the matrix to solidify. Cells (4000 per well) stably expressing vector control plasmid or B7-H3 overexpression plasmid were then seeded and allowed to grow for 8 days. The media was replaced with fresh growth media after 4 days. Acini ofMCF10A-Vector and MCF-10A-B7H3 were further fixed using a 1:1 mixture of methanol:acetone at −20°C for 10–12min. Acini were blocked with 2% FBS in PBS for 2 hours, then washed with PBS for 2 times. Acini were stain with anti-E-cadherin(Cell signaling) at 4°C overninght, and washed with PBS for 3 times. The Acini were stained with rhodamine-conjugated secondary antibody for 1–2 hr at room temperature in the dark, and the washed with PBS for 5 times. Slides were mounted with Prolong® Gold Antifade Reagent with DAPI.
Liu Z., Zhang W., Phillips J.B., Arora R., McClellan S., Li J., Kim J.H., Sobol R.W, & Tan M. (2018). Immunoregulatory Protein B7-H3 Regulates Cancer Stem Cell Enrichment and Drug Resistance through MVP-mediated MEK Activation. Oncogene, 38(1), 88-102.
+ Open protocol
+ Expand
4

Immunofluorescence Staining of Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were plated in Chamber Polystyrene Vessels (BD Falcon, Franklin Lakes, NJ, USA). Three days after culture in DMEM containing 10% FBS, the cells were fixed with 4% paraformaldehyde and permeabilized with 0.4% Triton X-100 for immunofluorescence staining. The cells were blocked with normal goat serum (Jackson ImmunoResearch Laboratories) and then incubated with primary antibodies at 1:100 dilutions against vimentin (Santa Cruz Biotechnology), cytokeratin (Santa Cruz Biotechnology) and CD45 (Abcam) overnight at 4°C. After washing with PBS, Alexa Fluor 488 (green)-and Alexa Fluor 594 (red)-labeled secondary antibodies (1:100; Proteintech) were applied in darkness at room temperature for 2 h. Nuclei were counterstained with 4′,6-diamidino-2phenylindole (DAPI, blue color) (1 μg/mL). The staining was examined using a fluorescence microscope (Zeiss).
Mi Y., Wang W., Lu J., Zhang C., Wang Y., Ying H, & Sun K. (2018). Proteasome-mediated degradation of collagen III by cortisol in amnion fibroblasts. Journal of molecular endocrinology, 60(2).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!