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Luciferase reporter kit

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The Luciferase Reporter Kit is a laboratory tool used to measure and analyze gene expression levels. It contains the necessary components, including the luciferase enzyme, to perform bioluminescence assays. The kit enables researchers to quantify the activity of specific promoters or regulatory elements in a variety of cell types and experimental conditions.

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Dual-luciferase reporter assay kit (115 citations) Double luciferase reporter gene detection kit (9 citations) Dual-Luciferase Reporter Assay System kit (5 citations) Dual-luciferase reporter gene kit (5 citations) Renilla-LumiTM Luciferase Reporter Gene Assay Kit (4 citations) Dual Luciferase Reporter Gene Assay Kit (RG027) (3 citations) Dual-luciferase reporter system kit (3 citations) Dual luciferase reporter gene test kit (2 citations) Double Luciferase Reporter Gene Assay Kit (2 citations) Dual-Lumi™ dual luciferase reporter gene detection kit (2 citations)

2 protocols using luciferase reporter kit

1

Luciferase Assay to Validate miRNA Targets

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The luciferase reporter plasmids (Bcl2-W and MRP4-W, containing wild-type binding site; Bcl2-M and MRP4-M, containing mutant binding site) were synthesized by the GenScript Co. (Piscataway, NJ, USA). HEK293T cells were co-transfected with the luciferase reporter plasmids and microRNAs, respectively. The Luciferase Reporter Kit (Beyotime Institute of Biotechnology, Beijing, China) was applied to detect the luciferase activity after 48 h in accordance with manufacturer's instructions.
Shang C., Ao C.N., Cheong C.C, & Meng L. (2019). Long Non-coding RNA CDKN2B Antisense RNA 1 Gene Contributes to Paclitaxel Resistance in Endometrial Carcinoma. Frontiers in Oncology, 9, 27.
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2

Luciferase Reporter Assay for miRNA Targeting

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The luciferase reporter plasmids (pmiR-SOCS6-W, containing wild-type binding site; pmiR-SOCS6-M, containing mutant binding site) were synthesized by the Genescript Co. (Piscataway, NJ, USA). HEK-293T cells were co-transfected with the luciferase reporter plasmids and microRNAs, respectively. The Luciferase Reporter Kit (Beyotime, Beijing, China) was applied to detect the luciferase activity 48 h later in accordance with instructions.
Liu Z., Xie D, & Zhang H. (2018). RETRACTED ARTICLE: Long noncoding RNA neuroblastoma-associated transcript 1 gene inhibits malignant cellular phenotypes of bladder cancer through miR-21/SOCS6 axis. Cell Death & Disease, 9(10), 1042.
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