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Enfuvirtide t 20

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Enfuvirtide (T-20) is a laboratory-grade chemical compound developed by Roche for use in research and development applications. It functions as a fusion inhibitor, targeting the viral envelope proteins of certain viruses to prevent fusion with host cells. The core purpose of Enfuvirtide is to serve as a research tool for scientists studying viral entry mechanisms and developing potential antiviral therapies.

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Lab products found in correlation

Facscanto 2, by BD (2 mentions) Cytofix cytoperm, by BD (2 mentions) Cd8 apc, by BD (2 mentions) Transwell plate, by Corning (2 mentions) Intracellular p24 gag pe, by Beckman Coulter (2 mentions) Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase hrp labeled goat anti rabbit igg, by Merck Group (1 mentions) Zidovudine azt, by Merck Group (1 mentions) Raltegravir ral, by Selleck Chemicals (1 mentions) Pha l, by Merck Group (1 mentions) Negative magnetic bead selection, by STEMCELL (1 mentions) Interleukin 2 (il 2), by Merck Group (1 mentions) Interleukin 2 (il 2), by Roche (1 mentions) Ril 2, by Roche (1 mentions) Colchicine, by Merck Group (1 mentions) Amd3100, by Merck Group (1 mentions) Tak 779, by Merck Group (1 mentions) Raltegravir ral, by Merck Group (1 mentions)

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Enfuvirtide (2 citations)

5 protocols using enfuvirtide t 20

1

Synthesis and Characterization of Antiviral Compounds

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FNC with a purity of 98.5% and 3TC with a purity of 99.6%, were synthesized by Dr. Jun-biao Chang, Zhengzhou University, China. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), sodium dodecyl sulfate (SDS), N, N-dimethylformamide (DMF), phytohemagglutinin (PHA-p), interleukin-2 (IL-2), Fc-specific anti-mouse IgG, horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG and zidovudine (AZT) were purchased from Sigma-Aldrich company. Raltegravir (RAL) was purchased from Selleck Chemicals. Nevirapine (NVP) was purchased from US Pharmacopeia. Enfuvirtide (T-20) was purchased from Roche Inc. RPMI-1640 media and fetal bovine serum (FBS) were purchased from Invitrogen, USA. FBS was heated at 56°C, 30 min to inactivate complements. Indinavir (IDV) was kindly gifted by Dr. Yu-Ye Li. Ficoll-Hypaque was purchased from Haoyang Biotechnonlogy Inc. Rabbit anti-p24 polyclonal antibody and mouse anti-p24 monoclonal antibody were prepared in our laboratory. Custom primers and fluorophore-labeled probes were synthesized by Invitrogen.
Wang R.R., Yang Q.H., Luo R.H., Peng Y.M., Dai S.X., Zhang X.J., Chen H., Cui X.Q., Liu Y.J., Huang J.F., Chang J.B, & Zheng Y.T. (2014). Azvudine, A Novel Nucleoside Reverse Transcriptase Inhibitor Showed Good Drug Combination Features and Better Inhibition on Drug-Resistant Strains than Lamivudine In Vitro. PLoS ONE, 9(8), e105617.
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2

CD4+ T cell-CD8+ T cell Interactions in HIV

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Three hours after infection, CD4+ T cells were mixed with autologous unstimulated or stimulated CD8+ T cells at 1:1 ratio in basal media at 5 million cells per ml. Two days after co-culture, enfuvirtide (T-20, Roche) was added into the culture at 10 μM to prevent further infection events except if the measurement was p24 ELISA. Three days after co-culture, cells were stained with CD8-APC (BD) first, fixed and permeabilized with Cytoperm/Cytofix (BD pharmingen), then stained for intracellular p24 Gag (PE, Coulter). Cells were analyzed by flow cytometry using FACS Canto II (BD). For measurement of viral growth, 5 μl of supernatant was taken from the co-culture at Days 0, 3 and 6, and subjected to p24 ELISA. For analysis of cell contact dependence, CD4+ and CD8+ T cells were placed in separate chambers of trans-well plates (0.4 μm, Costar).
Deng K., Pertea M., Rongvaux A., Wang L., Durand C.M., Ghiaur G., Lai J., McHugh H.L., Hao H., Zhang H., Margolick J.B., Gurer C., Murphy A.J., Valenzuela D.M., Yancopoulos G.D., Deeks S.G., Strowig T., Kumar P., Siliciano J.D., Salzberg S.L., Flavell R.A., Shan L, & Siliciano R.F. (2015). Broad CTL response is required to clear latent HIV-1 due to dominance of escape mutations. Nature, 517(7534), 381-385.
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3

CD4+ T cell-CD8+ T cell Interactions in HIV

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Three hours after infection, CD4+ T cells were mixed with autologous unstimulated or stimulated CD8+ T cells at 1:1 ratio in basal media at 5 million cells per ml. Two days after co-culture, enfuvirtide (T-20, Roche) was added into the culture at 10 μM to prevent further infection events except if the measurement was p24 ELISA. Three days after co-culture, cells were stained with CD8-APC (BD) first, fixed and permeabilized with Cytoperm/Cytofix (BD pharmingen), then stained for intracellular p24 Gag (PE, Coulter). Cells were analyzed by flow cytometry using FACS Canto II (BD). For measurement of viral growth, 5 μl of supernatant was taken from the co-culture at Days 0, 3 and 6, and subjected to p24 ELISA. For analysis of cell contact dependence, CD4+ and CD8+ T cells were placed in separate chambers of trans-well plates (0.4 μm, Costar).
Deng K., Pertea M., Rongvaux A., Wang L., Durand C.M., Ghiaur G., Lai J., McHugh H.L., Hao H., Zhang H., Margolick J.B., Gurer C., Murphy A.J., Valenzuela D.M., Yancopoulos G.D., Deeks S.G., Strowig T., Kumar P., Siliciano J.D., Salzberg S.L., Flavell R.A., Shan L, & Siliciano R.F. (2015). Broad CTL response is required to clear latent HIV-1 due to dominance of escape mutations. Nature, 517(7534), 381-385.
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4

Isolation and Stimulation of CD4+ T Cells

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CD4 T cells were isolated from PBMC by negative magnetic bead selection (StemCell), resulting in an untouched population defined as CD3+CD4+/-CD8-CD14-CD19- (purity routinely over 95%). Cells were stimulated for 36–40 hr in RPMI with PHA-L (10μg/ml, Sigma) and IL-2 (50U/ml), then washed and maintained for 6–7 days in RPMI with IL-2 (100U/ml). In some experiments, ARVs (T20 (7.5μg/ml) + AZT (1μM)) were added. Enfuvirtide (T-20), Zidovudine (AZT), and IL-2 (Lahm and Stein, 1985 (link)) were obtained through NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH: human rIL-2 from Dr. Maurice Gately, Hoffmann - La Roche Inc.
Baxter A.E., Niessl J., Fromentin R., Richard J., Porichis F., Charlebois R., Massanella M., Brassard N., Alsahafi N., Delgado G.G., Routy J.P., Walker B.D., Finzi A., Chomont N, & Kaufmann D.E. (2016). Single-cell characterization of viral translation-competent reservoirs in HIV-infected individuals. Cell host & microbe, 20(3), 368-380.
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5

Polyanionic Carbosilane Dendrimers Evaluation

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Polyanionic carbosilane dendrimers, third-generation G3-S16 with 16 sulfated end groups (C256H508N48Na16O64S16Si29; MW: 6,978.41 g/mol) and second-generation G2-NF16 with naphthylsulfonated end groups (C184H244N24Na16O56S16Si13; MW: 4,934.02 g/mol), were prepared according to reported methods.34 Several reagents and ARV were used as controls: zidovudine (AZT; GSK, GlaxoSmithKline plc, London, UK), enfuvirtide (T-20; Hoffman-La Roche Ltd., Basel, Switzerland), atazanavir (ATV; Bristol-Myers Squibb, New York, NY, USA), and raltegravir (RAL; Merck Millipore, Billerica, MA, USA); Suramin, a polyanionic compound that could mimic the function of dendrimers; CXCR4 chemokine receptor antagonist AMD3100, CCR5 receptor antagonist TAK-779, and colchicine, a microtubule polymerization inhibitor (all from Sigma-Aldrich Co., St Louis, MO, USA).
Vacas-Córdoba E., Maly M., De la Mata F.J., Gómez R., Pion M, & Muñoz-Fernández M.Á. (2016). Antiviral mechanism of polyanionic carbosilane dendrimers against HIV-1. International Journal of Nanomedicine, 11, 1281-1294.
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