Penn strep

Manufactured by Thermo Fisher Scientific

Sourced in United States, Gabon

Penn/Strep is a solution containing penicillin and streptomycin, two commonly used antibiotics in cell culture applications. It is used to prevent bacterial contamination in cell culture media and samples.

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Lab products found in correlation

61 protocols using penn strep

Maintenance of Cell Lines for Experiments

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NIH/3T3 cells (ATCC, CRL1658) were maintained at 37°C in 5% CO2 in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% Bovine Calf Serum (Sigma) and 1% Penn/Strep (Gibco). 293FT cells (Thermo, R700-07) were maintained at 37°C in 5% CO2 in high glucose Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% Fetal Bovine Serum (Tissue Culture Biologicals) and 1% Penn/Strep (Gibco). All lines were passaged at ~80–85% confluence. Immortalized brown pre-adipocytes^{40 (link)} were maintained at 37°C in 5% CO2 in DMEM/F-12 Glutamax, 10% FBS, 1% Penn/Strep (Gibco). NIH/3T3 and 293FT cell lines were low passage and not tested for mycoplasma. Immortalized brown pre-adipocytes tested negative for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza).

Emmett M.J., Lim H.W., Jager J., Richter H.J., Adlanmerini M., Peed L.C., Briggs E.R., Steger D.J., Ma T., Sims C.A., Baur J.A., Pei L., Won K.J., Seale P., Gerhart-Hines Z, & Lazar M.A. (2017). Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge. Nature, 546(7659), 544-548.

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Culturing and Preparing MDCK Cells

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Madin-Darby Canine kidney (MDCK) cells (ATCC, CCL-34) were maintained in Dulbecco’s Modified Eagle Medium + GlutaMAX (D MEM, Gibco) supplemented with 10% fetal bovine serum (FBS) and 1% Penn/strep (Gibco). MDCK-SIAT1 cells were maintained in D MEM supplemented with 1 mg/ml G418 Geneticin (Gibco), 10% FBS, and 1% Penn/strep. The hCK cell line was maintained in Minimum Essential Media + GlutaMAX (MEM, Gibco) supplemented with 5% newborn calf serum, 1% blasticidin, 1% Penn/strep and 0.02% puromycin. Cells were split at 90% confluency 1:10 into T75cm² flasks and were kept at 37 °C, 5% CO₂. After desired passage, the culture media was replaced with serum free media in order to reduce the detection of serum glycans, and the flasks were allowed to incubate for 24 h. Cells were released via trypsinization and centrifuged to pellet. The pellet was reconstituted in phosphate buffered saline (PBS) for downstream analysis.

Byrd-Leotis L., Jia N., Matsumoto Y., Lu D., Kawaoka Y., Steinhauer D.A, & Cummings R.D. (2022). Sialylated and sulfated N-Glycans in MDCK and engineered MDCK cells for influenza virus studies. Scientific Reports, 12, 12757.

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Maintenance of Cell Lines for Experiments

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Cell Culture Protocols for Cancer Research

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A20 and B16F10 cells were purchased from ATCC. OCI-LY3 and SU-DHL-4 were generously shared by Dr. Han Tun, with genomic profiling performed November 2022 (GENOMIC). A20 cells were cultured in RPMI (Fisher-Scientific) supplemented with 10% FBS (VWR) and 1% Penn-Strep (Life Technologies). OCI-LY3 and SU-DHL-4 cells were cultured in RPMI (Fisher-Scientific) supplemented with 20% FBS (VWR) and 1% Penn-Strep (Life Technologies). B16F10 cells were cultured in DMEM (Fisher-Scientific) supplemented with 10% FBS (VWR) and 1% Penn-Strep (Life Technologies). All cell lines were maintained at 37°C in humidified conditions with 5% CO₂. At the beginning of the study, cells were expanded, stocks made, and thawed vials were maintained in culture for no more than 3 weeks.

Von Roemeling C.A., Doonan B.P., Klippel K., Schultz D., Hoang-Minh L., Trivedi V., Li C., Russell R.A., Kanumuri R.S., Sharma A., Tun H.W, & Mitchell D.A. (2023). Oral IRAK-4 Inhibitor CA-4948 Is Blood-Brain Barrier Penetrant and Has Single-Agent Activity against CNS Lymphoma and Melanoma Brain Metastases. Clinical Cancer Research, 29(9), 1751-1762.

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Cultivation of Human Cell Lines

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Human embryonic kidney cell line 293, human hepatocellular carcinoma cell lines HepG2 and Hep3B, human liver adenocarcinoma cell line SK-HEP-1, human prostate carcinoma cell line LNCaP, human colon adenocarcinoma cell line Caco-2, human breast adenocarcinoma cell line MCF-7, and human lung carcinoma cell line A-549 were purchased from the American Type Culture Collection (ATCC, Manassas, VA). The human hepatocellular carcinoma cell line HuH-7 was a kind gift from Dr. Jianming Hu (Penn State Hershey College of Medicine, Hershey, PA). Hep3B, HEK293, A-549, and HuH-7 cells were cultured in DMEM (Gibco, Carlsbad, CA) supplemented with 10% FBS (Atlanta Biologicals, Lawrenceville, GA) and 1% penn/strep (Gibco). HepG2 and Caco-2 cells were cultured in DMEM supplemented with 10% FBS, 1% penn/strep, and 1% non-essential amino acids (Lonza, Basel, Switzerland). SK-HEP-1 and LNCaP cells were cultured in RPMI 1640 (Gibco) supplemented with 10% FBS, 1% penn/strep, and 1% NEAA. MCF-7 cells were cultured in RPMI 1640 supplemented with 10% FBS and 1% penn/strep. All cells were grown and maintained at 37°C with 5% CO₂.

Dluzen D.F., Sun D., Salzberg A.C., Jones N., Bushey R.T., Robertson G.P, & Lazarus P. (2015). Regulation of UGT1A1 expression and activity by miR-491-3p*. Drug metabolism reviews, 47(3), 320-334.

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Embryoid Body Formation and Cardiomyocyte Differentiation

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To generate embryoid bodies (EBs), suspension of each cell line at a concentration of 10 × 10⁴cells per milliliter in differentiating medium (Knock-out DMEM supplemented with 15% FBS (Gibco), 1% non-essential amino acids, 1% Glutamax, 1% Penn-Strep, 0.1 mm β-mercaptoethanol) was deposited in 20 μL hanging drops in Petri dishes. After 2 days, the suspension-cultured cells were plated onto 0.2% gelatin-coated dishes in cardiomyocyte differentiation media (CMD) containing DMEM (BI), 15% FBS (Gibco), 1% Penn-Strep, 1% non-essential amino acids (Gibco), 1% Glutamine (Gibco), 0.1 mm β-mercaptoethanol and 1 mm Ascorbic Acid (Sigma) for continued differentiation. Cell culture media was changed every other day and contracting patches of cells were collected at indicated time points.

Wang S., Zhang J., Ding Y., Zhang H., Wu X., Huang L., He J., Zhou J, & Liu X.M. (2022). Dynamic Transcriptome Profiling Reveals LncRNA-Centred Regulatory Networks in the Modulation of Pluripotency. Frontiers in Cell and Developmental Biology, 10, 880674.

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Culturing HepG2 and WI-38 Cell Lines

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HepG2 (ATCC, human male) cells were cultured in a 37°C 5% CO₂ and 20% O₂ humidified incubator with Dulbecco’s Modified Eagles Medium (DMEM, Gibco) supplemented with 10% Fetal Bovine Serum (FBS, Thermo Fisher) and 1% penicillin/streptomycin (Penn/Strep, Thermo Fisher). WI-38 (Coriell Institute, human female) cells were cultured in a 37°C 5% CO₂ and 20% O₂ humidified incubator with DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco), 0.5% Penn/Strep (Gibco), sodium pyruvate (Gibco), and non-essential amino acids (Gibco).

Occean J.R., Yang N., Sun Y., Dawkins M.S., Munk R., Belair C., Dar S., Anerillas C., Wang L., Shi C., Dunn C., Bernier M., Price N.L., Kim J.S., Cui C.Y., Fan J., Bhattacharyya M., De S., Maragkakis M., deCabo R., Sidoli S, & Sen P. (2023). Gene body DNA hydroxymethylation restricts the magnitude of transcriptional changes during aging. bioRxiv.

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Culturing Mouse and Drosophila Cell Lines

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Low-passage E14 mouse embryonic stem cells (mESCs) were grown in
knockout DMEM (Invitrogen, 10829-018) with 15% ESC grade FBS (EMD
Millipore, ES-009-B), 1× L-glutamine (EMD Millipore, TMS-002-C),
1× ES-grade Penicillin/streptomycin (EMD Millipore, TMS-AB2-C),
1× non-essential amino acid (EMD Millipore, TMS-001-C), 0.1%
2-mercaptoethanol, 1× Leukemia Inhibitory Factor (LIF, EMD
Millipore, ESG1107) in 37 °C, 5% CO₂ incubator.
One hour prior to harvest, ∼60-70% confluency cells were
incubated with fresh mESC media. Mouse C3H10T1/2 cells were grown in the
growth media (10% FBS (Hyclone, SH30071.03), Penn/ Strep (Gibco,
15140-122), DMEM (Gibco, 11965-118)) in 37 °C, 5%
CO₂ incubator. Drosophila Schneider 2 (S2)
cells were grown in growth media containing 10% heat inactivated FBS
(Hyclone, SH30071.03), Penn/Strep (Gibco, 15140-122), and
Schneider's Drosophila medium (Gibco, 21720024)) at
room temperature in ambient CO₂.

Shi Z., Fujii K., Kovary K.M., Genuth N.R., Röst H.L., Teruel M.N, & Barna M. (2017). Heterogeneous ribosomes preferentially translate distinct subpools of mRNAs genome-wide. Molecular cell, 67(1), 71-83.e7.

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Culturing U2OS and BCBL-1 cells

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U2OS cells (HTB‐96) were obtained from ATCC and maintained in DMEM medium (Gibco 11995‐065) with 10% EV‐free Fetal Bovine Serum (FBS VWR 97068‐085), 20 mM of L‐Glutamine (Gibco 25030‐081), and 100 U/mL PennStrep (Gibco 15140‐122). BCBL‐1 cells were from Don Ganem (Renne et al., 1996 ) and maintained in RPMI medium (Gibco 11875‐093) with 10% EV‐free FBS, 20 mM of L‐Glutamine (Gibco 25030‐081), and 100 U/mL PennStrep (Gibco 15140‐122). To make EV‐free FBS, PEG‐8000 was added to FBS to a final concentration of 40 mg/mL and incubated at 4°C overnight, followed by centrifugation at 1000 g for 1 h. The supernatant (EV‐free FBS) was used in cell culture. For routine maintenance, cells were trypsinized (Gibco 25300‐054) upon 80% confluency, and 5 × 10⁶ cells were seeded in a T175 flask.

Zhou Y., Yuan R., Cone A.S., Shifflett K.W., Arias G.F., Peng A., Chambers M.G., McNamara R.P., Willcox S., Landis J.T., Pan Y., Griffith J, & Dittmer D.P. (2023). Large‐scale heparin‐based bind‐and‐elute chromatography identifies two biologically distinct populations of extracellular vesicles. Journal of Extracellular Vesicles, 12(6), 12327.

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Immortalized Cell Lines for Research

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Tissue preparation, immunostaining, and semiquantitative evaluation were done as described.⁴⁰ (link) Lymphoblastoid cell lines were maintained after EBV immortalization. Fibroblasts were cultured from a 3 mm punch skin biopsy. In addition to these cells, we also purchased a control immortalized PBMC (ATCC #CRL5959) of a normal individual (a 58-year-old male, European descent) and two control primary fibroblasts (Coriell #GM07753 and #GM07492) of apparently healthy individuals (both are 17-year-old males, European descent, named CTL-17Ma and -17Mb, respectively). All the immortalized PBMCs were maintained in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 15% fetal bovine serum (FBS) and 4 mM L-Glutamine (Thermo Scientific). For prevention against bacteria, fungi, and mycoplasma, the media also contained Penn Strep (Thermo Scientific), Normocin (Invivogen), Nystatin suspension, and Amphotericin B solution (Sigma Aldrich) according to manufacturers’ instructions. The primary fibroblasts were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) containing high glucose and GlutaMAX (Thermo Scientific) and supplemented with 15% FBS and 1% Penn Strep. Cells were incubated at 37°C in a humidified CO₂ incubator. The immortalized PBMCs of less than 30 passages and primary fibroblasts of less than 20 passages were employed in the experiments.

Xu Z., Lo W.S., Beck D.B., Schuch L.A., Oláhová M., Kopajtich R., Chong Y.E., Alston C.L., Seidl E., Zhai L., Lau C.F., Timchak D., LeDuc C.A., Borczuk A.C., Teich A.F., Juusola J., Sofeso C., Müller C., Pierre G., Hilliard T., Turnpenny P.D., Wagner M., Kappler M., Brasch F., Bouffard J.P., Nangle L.A., Yang X.L., Zhang M., Taylor R.W., Prokisch H., Griese M., Chung W.K, & Schimmel P. (2018). Bi-allelic Mutations in Phe-tRNA Synthetase Associated with a Multi-system Pulmonary Disease Support Non-translational Function. American Journal of Human Genetics, 103(1), 100-114.

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