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L glutamine l glu

Manufactured by Lonza
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Sourced in Netherlands, Switzerland, Belgium

L-glutamine (L-glu) is a non-essential amino acid that is naturally produced by the human body. It is an important component in various cellular processes and plays a crucial role in maintaining the integrity of the gastrointestinal tract and supporting immune function.

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Lab products found in correlation

Non essential amino acids (neaa), by Lonza (4 mentions) Nahco3, by Lonza (4 mentions) Hepes, by Lonza (4 mentions) Fetal bovine serum (fbs), by Greiner (2 mentions) Penicillin p, by Lonza (2 mentions) Streptomycin s, by Lonza (2 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (2 mentions) L glu, by Thermo Fisher Scientific (2 mentions) Dulbecco modified eagle medium (dmem), by Lonza (2 mentions) Streptomycin str, by Lonza (2 mentions) Lsrii cytometer, by BD (1 mentions) Fcs express v3, by De Novo Software (1 mentions) L glu, by Lonza (1 mentions) Penicillin pen, by Lonza (1 mentions) Streptomycin pen strep, by Lonza (1 mentions) Penicillin, by Lonza (1 mentions)

Spelling variants of this product

L-glutamine (1419 citations) Glutamine (187 citations) L-Glu (6 citations)

6 protocols using l glutamine l glu

1

MDCK Cell Culture Conditions

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Madin-Darby canine kidney (MDCK) cells (ATCC) were cultured in Eagle’s minimal essential medium (EMEM, Lonza Benelux BV, Breda, the Netherlands) supplemented with 10% fetal bovine serum (FBS) (Greiner), 100U/ml penicillin (P, Lonza), 100U/ml streptomycin (S, Lonza), 2mM L-glutamine (L-glu, Lonza), 1.5mg/ml sodium bicarbonate (NaHCO3, Lonza), 10mM Hepes (Lonza) and 1X non-essential amino acids (NEAA, Lonza).
Richard M., Herfst S., van den Brand J.M., Lexmond P., Bestebroer T.M., Rimmelzwaan G.F., Koopmans M., Kuiken T, & Fouchier R.A. (2015). Low Virulence and Lack of Airborne Transmission of the Dutch Highly Pathogenic Avian Influenza Virus H5N8 in Ferrets. PLoS ONE, 10(6), e0129827.
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2

Cell Culture Conditions for Viral Studies

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Madin-Darby canine Kidney (MDCK) cells (ATCC) were cultured in Eagle’s minimal essential medium (EMEM, Lonza Benelux BV, Breda, the Netherlands) supplemented with 10% foetal bovine serum (FBS) (Greiner), 100 U ml−1 penicillin (PEN, Lonza), 100 U ml−1 streptomycin (STR, Lonza), 2 mM L-glutamine (L-glu, Lonza), 1.5 mg ml−1 sodium bicarbonate (NaHCO3, Lonza), 10 mM Hepes (Lonza) and 1X non-essential amino acids (NEAA, Lonza). 293T cells (ATCC) were cultured in Dulbecco modified Eagle’s medium (DMEM, Lonza) supplemented with 10% FBS, 100 U ml−1 PEN, 100 U ml−1 SRT, 2mM L-glu, 1 mM sodium pyruvate (Gibco) and 1X NEAA. Human airway epithelia reconstituted in vitro (MucilAirTM, EP02MP) were purchased from Epithelix Sàrl (Switzerland).
Richard M., van den Brand J.M., Bestebroer T.M., Lexmond P., de Meulder D., Fouchier R.A., Lowen A.C, & Herfst S. (2020). Influenza A viruses are transmitted via the air from the nasal respiratory epithelium of ferrets. Nature Communications, 11, 766.
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3

Melanoma Cell Line Characterization

Cited 2 times
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Primary melanoma cell lines used in this study were previously
described19 (link), 20 (link). All cell lines (primary and
commercial) were incubated in humidified incubators supplied with 5%
CO2 and cultured in RPMI1640 medium supplemented with 10%
FBS, 1% 10K/10K Penicillin-Streptomycin (P/S Solution, Lonza, Basel,
Switzerland) and 1% 200mM L-Glutamine (L-Glu, Lonza, Basel,
Switzerland). Two exceptions where commercial cell lines Mewo and SK-MEL-28
which were cultured in DMEM with the same supplements. Melanocytic progeny of
primary melanoma cell lines (PrMCLs) were investigated by staining for S100,
MART-1, gp100 and GD2 expression using polyclonal rabbit anti-S100, monoclonal
A-103, HMB-45 (Invitrogen, Carlsbad, CA, USA) and R-24 (a kind gift of Dr. Aliza
Adler) antibodies, and flow cytometry based (FACS) detection of HMW-MMA (CSPG4)
(R&D Systems Minneapolis, MN, USA) (Supplementary Table 1).
Samples were analyzed by FACS on a Becton Dickinson LSR II cytometer. Data
analysis was performed using FCS express V.3 (De Novo Software).
Senses K.M., Ghasemi M., Akbar M.W., Isbilen M., Fallacara A.L., Frankenburg S., Schenone S., Lotem M., Botta M, & Gure A.O. (2016). Phenotype-based variation as a biomarker of sensitivity to molecularly targeted therapy in melanoma †Electronic supplementary information (ESI) available. See DOI: 10.1039/c6md00466k. MedChemComm, 8(1), 88-95.
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4

Cell Culture Preparation for Research

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Madin-Darby canine kidney (MDCK) cells (ATCC) were cultured in Eagle’s minimal essential medium (EMEM) (Lonza) supplemented with 10% fetal bovine serum (FBS) (Greiner), 100 U/ml penicillin (PEN) (Lonza), 100 U/ml streptomycin (STR) (Lonza), 2 mM l-glutamine (l-Glu) (Lonza), 1.5 mg/ml sodium bicarbonate (NaHCO3) (Lonza), 10 mM HEPES (Lonza) and 1× nonessential amino acids (NEAA) (Lonza). 293T cells were cultured in Dulbecco modified Eagle’s medium (DMEM) (Lonza) supplemented with 10% FBS, 100 U/ml PEN, 100 U/ml STR, 2 mM l-Glu, 1 mM NaHCO3, and 1× NEAA. Vero cells were cultured in Iscove’s modified Dulbecco’s medium (IMDM) (Lonza) supplemented with 10% FBS, 100 U/ml PEN, 100 mg/ml STR, and 2 mM l-Glu.
Herfst S., Mok C.K., van den Brand J.M., van der Vliet S., Rosu M.E., Spronken M.I., Yang Z., de Meulder D., Lexmond P., Bestebroer T.M., Peiris J.S., Fouchier R.A, & Richard M. (2018). Human Clade 2.3.4.4 A/H5N6 Influenza Virus Lacks Mammalian Adaptation Markers and Does Not Transmit via the Airborne Route between Ferrets. mSphere, 3(1), e00405-17.
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5

MDCK and 293T Cell Culture Conditions

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Madin-Darby canine Kidney (MDCK) cells (ATCC) were cultured in Eagle’s minimal essential medium (EMEM, Lonza Benelux BV, Breda, the Netherlands) supplemented with 10% fetal bovine serum (FBS) (Greiner), 100 U/ml penicillin (P, Lonza), 100 U/ml streptomycin (S, Lonza), 2 mM L-glutamine (L-glu, Lonza), 1.5 mg/ml sodium bicarbonate (NaHCO3, Lonza), 10mM Hepes (Lonza) and 1X non-essential amino acids (NEAA, Lonza). 293T cells were cultured in Dulbecco modified Eagle’s medium (DMEM, Lonza) supplemented with 10% FBS, 100 U/ml P, 100 U/ml S, 2 mM L-glu, 1mM sodium pyruvate (Gibco) and 1X NEAA.
Richard M., Herfst S., van den Brand J.M., de Meulder D., Lexmond P., Bestebroer T.M, & Fouchier R.A. (2017). Mutations Driving Airborne Transmission of A/H5N1 Virus in Mammals Cause Substantial Attenuation in Chickens only when combined. Scientific Reports, 7, 7187.
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6

Syngeneic Glioma Cell Cultures

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GL261 glioma cells were kindly provided by Dr. Ilker Eyüpoglu from the University of Erlangen (Germany). CT2A cells were a generous gift from Prof. Holger Gerhardt from the Vesalius Research Center (VIB, Leuven, Belgium) with MTA obtained from Prof. Thomas N. Seyfried (Boston College, USA). Both C57BL/6 syngeneic tumor cell cultures were maintained at 37 °C under 5% CO2 in DMEM supplemented with 10% heat-inactivated fetal calf serum (FCS), 100 U/ml penicillin, 100 µg/ml streptomycin (pen/strep) and 2 mM L-glutamine (L-Glu) (all from Lonza, Verviers, Belgium).
Belmans J., Van Woensel M., Creyns B., Dejaegher J., Bullens D.M, & Van Gool S.W. (2017). Immunotherapy with subcutaneous immunogenic autologous tumor lysate increases murine glioblastoma survival. Scientific Reports, 7, 13902.
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