Sacrifice was performed as above excluding whole body perfusion with formalin. One heart was fixed in 10% neutral buffered formalin and 3% glutaraldehyde in sodium cacodylate buffer (pH 7.4) (Electron Microscopy Sciences). Samples underwent conventional processing for electron microscopy by the Comparative Pathology and Mouse Phenotyping Shared Resource (The Ohio State University, Columbus, OH). Trimmed sections (50 nm) were placed on copper grids and stained with filtered lead citrate/sodium citrate solution (Electron Microscopy Sciences) for 5 minutes. Grids were imaged on a JEOL JEM-1400 TEM (JEOL USA, Inc.) and imaged with an Olympus SIS Veleta 2K camera (Olympus Soft Imaging Solutions GmbH).
Sis veleta 2k camera
Manufactured by Olympus
Sourced in United States, Germany
The SIS Veleta 2K camera is a high-resolution digital imaging solution for laboratory applications. It features a 2048 x 2048 pixel sensor and can capture images at up to 30 frames per second. The camera is designed for use in a variety of microscopy techniques, including brightfield, darkfield, and fluorescence imaging.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sis veleta 2k camera
1
Electron Microscopy of Heart Tissue
2
Transmission Electron Microscopy of Glutaraldehyde-Fixed Tissue
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Due to resources constraint, the glutaraldehyde-fixed tissue from only 4 dogs (2 control and 2 treated) were processed for electronic microscopy. After post-fixation in 1% osmium tetroxide, the specimens were serially dehydrated, infiltrated in an acetone/epoxy plastic, and embedded in plastic. The plastic blocks were sectioned to a silver-grey interference color (55–60 nm) and placed on copper mesh grids. The sections were stained with filtered lead citrate/sodium citrate solution (Electron Microscopy Sciences, Inc., Hatfield, PA, USA). Grids were imaged on a JEOL JEM-1400 TEM (JEOL USA, Inc., Peabody, MA, USA) and representative images were photographed with an Olympus SIS Veleta 2K camera (Olympus Soft Imaging Solutions GmbH, Muenster, Germany.
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