Fungal biomass was harvested by vacuum filtration, washed with precooled, distilled and sterile water, and shock frozen and ground in liquid nitrogen. For extraction of genomic DNA, plasmid DNA and RNA, purification kits (Wizard Genomic DNA Purification Kit, PureYield Plasmid Midiprep System and RNeasy plant kit, respectively, all from Promega) were used according to the manufacturer’s specifications. Standard methods were used for electrophoresis, blotting and hybridization of nucleic acids (Sambrook and Russell, 2001 ).
Rneasy plant kit
Manufactured by Promega
The RNeasy Plant Kit is a nucleic acid extraction system designed to isolate high-quality total RNA from a wide variety of plant tissues and cells. The kit utilizes a silica-based membrane technology to efficiently capture and purify RNA, allowing for downstream applications such as RT-PCR, Northern blotting, and microarray analysis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using rneasy plant kit
1
Fungal Biomass Harvesting and Nucleic Acid Extraction
2
Nucleic Acid Extraction from Fungal Biomass
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Fungal biomass was harvested by vacuum filtration, washed with precooled, distilled and sterile water, and shock frozen and ground in liquid nitrogen. For extraction of genomic DNA, plasmid DNA and RNA, purification kits (Wizard Genomic DNA Purification Kit, PureYield Plasmid Midiprep System and RNeasy plant kit, respectively, all from Promega) were used according to the manufacturer's specifications. Standard methods were used for electrophoresis, blotting and hybridization of nucleic acids (Sambrook and Russell, 2001 ).
3
Fungal Nucleic Acid Extraction Protocol
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Fungal mycelia were harvested by filtration, washed with distilled cold water, frozen and ground under liquid nitrogen. For extraction of genomic DNA, plasmid DNA and RNA, purification kits (Wizard Genomic DNA Purification Kit, PureYield Plasmid Midiprep System and RNeasy plant kit, respectively, all from Promega) were used according to the manufacturer's protocol. Standard methods were used for electrophoresis, blotting and hybridization of nucleic acids.
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