Human g csf

Manufactured by Thermo Fisher Scientific

Sourced in United States

Human G-CSF is a recombinant protein that functions as a growth factor for neutrophil progenitor cells. It stimulates the production, differentiation, and activation of neutrophils, which are a type of white blood cell important for immune defense.

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Lab products found in correlation

Human gm csf, by Thermo Fisher Scientific (2 mentions) Hsc007, by R&D Systems (1 mentions) Murine m csf, by Thermo Fisher Scientific (1 mentions) Murine gm csf, by Thermo Fisher Scientific (1 mentions) Hsc006, by R&D Systems (1 mentions) Penicillin streptomycin, by Lonza (1 mentions) 48 well plate, by BD (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Human m csf, by Thermo Fisher Scientific (1 mentions) Human scf, by Thermo Fisher Scientific (1 mentions) Cd34 cells, by Miltenyi Biotec (1 mentions) L glutamine, by Lonza (1 mentions)

Close spelling variants of this product

G-CSF (149 citations) G-CSF Recombinant Human Protein (2 citations) Human G-CSF Instant ELISA Kit (2 citations)

3 protocols using human g csf

Hematopoietic Progenitor Cell Differentiation

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Hematopoietic CD41⁺ progenitor cells obtained from iPSC differentiation (8 days after start of EB culture) were seeded at a concentration of 20,000–50,000/ml in basic and complete methylcellulose medium (HSC006 with no cytokines added and HSC007 containing IL-6, erythropoietin, SCF, and IL-3, respectively; R&D Systems). HSC007 was additionally supplemented with 20 ng/ml human G-CSF and 20 ng/ml murine M-CSF, while HSC006 was supplemented with 50 ng/ml murine GM-CSF (all Peprotech). Cells were grown for 7–10 days and colonies composed of more than 50 cells were counted.

Mucci A., Kunkiel J., Suzuki T., Brennig S., Glage S., Kühnel M.P., Ackermann M., Happle C., Kuhn A., Schambach A., Trapnell B.C., Hansen G., Moritz T, & Lachmann N. (2016). Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency. Stem Cell Reports, 7(2), 292-305.

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Generation of MDSCs from CD34+ Cells

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Human CB was provided from the Catholic Hematopoietic Stem Cell Bank after written informed consent given by normal full-term pregnant women. For MDSCs generation, isolated CD34⁺ cells (Miltenyi Biotec, Bergisch Gladbach, Germany) were cultured in a 48-well plate (BD Falcon, Bedford, MA) at 1 × 10⁵ cells/ well with 1 ml of IMDM containing 10% FBS (Gibco, Grand Island, NY, United States), 10% penicillin–streptomycin (100 U/ml; Lonza Walkersville, MD, United States), 2 mM L-glutamine (Lonza Walkersville) (10% complete medium), 100 ng/ml human GM-CSF (300–03, PeproTech, Rocky Hill, NJ, United States), 100 ng/ml human G-CSF (300–23, PeproTech), or 100 ng/ml human M-CSF (300–25, PeproTech) and 50 ng/ml human SCF (300–07, PeproTech). After incubation for 7 days, the cells were removed from the 48 well plate and centrifuged at 1,300 rpm for 5 min. After one wash with serum free IMDM, the cells were cultured for 2 weeks and media was changed every 7 days. From weeks 4–6, the cells were cultured at a higher density (5 × 10⁵ cells/well). Media was changed every 7 days throughout 6 weeks of the culture.

Park M.Y., Lim B.G., Kim S.Y., Sohn H.J., Kim S, & Kim T.G. (2019). GM-CSF Promotes the Expansion and Differentiation of Cord Blood Myeloid-Derived Suppressor Cells, Which Attenuate Xenogeneic Graft-vs.-Host Disease. Frontiers in Immunology, 10, 183.

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Hematopoietic Progenitor Differentiation on OP9

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CD34⁺ hematopoietic progenitor cells were purified from human cord blood by Ficoll-based density gradient centrifugation followed by CD34 magnetic beads selection (Miltenyi Biotec). OP9 mouse bone marrow stromal cells were cultured in the 6-well plate at 10⁴ cells/well the day before CD34⁺ purification. On Day 0, CD34⁺ cells were co-cultured with OP9 cells at 10⁵ cells/well in Iscove’s modified Dulbecco’s medium supplemented with 20% FBS, 1% antibiotics, 20 ng/mL human GM-CSF and 100 ng/mL human G-CSF (PeproTech). After overnight culture, tumor-conditioned media (TCM) from RPMI8226 human myeloma cell lines was added at 10% with DMSO or 100 nM BMS-595. On Day 3 and 6, fresh media containing 20 ng/mL GM-CSF, 100 ng/mL G-CSF, 10% TCM, and DMSO or 100 nM BMS-595 was added in the wells. On Day 9, the cells were collected and analyzed by flow cytometry.

Hashimoto A., Gao C., Mastio J., Kossenkov A., Abrams S.I., Purandare A.V., Desilva H., Wee S., Hunt J., Jure-Kunkel M, & Gabrilovich D.I. (2018). Inhibition of casein kinase 2 disrupts differentiation of myeloid cells in cancer and enhances the efficacy of immunotherapy in mice. Cancer research, 78(19), 5644-5655.

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