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α stat1

Manufactured by Cell Signaling Technology
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α-STAT1 is a primary antibody that recognizes the phosphorylated form of STAT1 protein. STAT1 is a key transcription factor involved in the cellular response to interferons and other cytokines. The α-STAT1 antibody can be used to detect the activated, phosphorylated state of STAT1 in various experimental applications.

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Lab products found in correlation

α actin, by Merck Group (2 mentions) αpstat1, by Cell Signaling Technology (2 mentions) Semi dry apparatus, by Bio-Rad (1 mentions) Rabbit α gapdh, by Cell Signaling Technology (1 mentions) α tubulin, by Santa Cruz Biotechnology (1 mentions) α gfp jl 8, by Takara Bio (1 mentions) α dsred, by Takara Bio (1 mentions) α gapdh, by Santa Cruz Biotechnology (1 mentions) α h3k27me3, by Cell Signaling Technology (1 mentions) Eed226, by Selleck Chemicals (1 mentions) Gsk503, by Selleck Chemicals (1 mentions) α p38 mapk, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

STAT1 (305 citations)

4 protocols using α stat1

1

Western Blot Analysis of Cellular Fractions

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Equivalent cytosolic and nuclear fractions were resolved in 10% linear mini sodium dodecyl sulfate polyacrylamide gels (SDS-PAGE, 8.0 × 6.5 × 0.1 cm) at 180 V for 60 min. Proteins were transferred to 0.2 μm polyvinylidene difluoride (PVDF) membranes at 20 V for 40 min with a Semi-dry apparatus (BioRad), and protein transfer was confirmed by Ponceau-S staining. The membranes were blocked with 5% (w/v) skim milk in Tris-buffered saline with Tween-20 (TBST; 50 mM Tris, 150 mM NaCl, 0.05% Tween 20, pH 7.4) and probed with various primary antibodies. Primary antibodies were: in-house produced rabbit anti-reovirus, rabbit α-GAPDH (Cell Signaling, cat#2118), α-ISG15 (Rockland, cat#200-401-438), and α-IFIT (Abcam, cat#ab55837); goat α-Mx1 (Santa Cruz #sc-34128); and mouse α-Actin (Sigma, cat#A5441), and α-STAT1 (Cell Signaling, cat#9176). Appropriate secondary horseradish peroxidase (HRP)-conjugated horse anti-mouse or goat anti-rabbit (Cell Signaling, cat#7076, cat#7074, respectively), or rabbit anti-goat (Zymed, cat#81-1620) were used to detect immune complexes. Bands were developed by enhanced chemiluminescence and imaged with an Alpha Innotech FluorChemQ MultiImage III instrument.
Ezzati P., Komher K., Severini G, & Coombs K.M. (2015). Comparative proteomic analyses demonstrate enhanced interferon and STAT-1 activation in reovirus T3D-infected HeLa cells. Frontiers in Cellular and Infection Microbiology, 5, 30.
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2

Antibody Panel for LGTV Detection

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The following antibodies were used: α-actin (A5441, Sigma); α-tubulin (sc-12462, Santa Cruz); α-GFP (JL-8, 632381, Clontech); α-dsRed (632496, Clontech); α-V5 (R960-25, Invitrogen); α-STAT1 (9172S, Cell Signaling Technology); α-STAT1-P (9167S, Cell Signaling Technology); α-LGTV E and NS1 (provided by Dr. C. Schmaljohn, USAMRIID); α-LGTV NS3 [45 (link)]; α-IFIT2 and α-IFIT3 (provided by Dr. S. Chattopadhyay, University of Toledo).
Izuogu A.O., McNally K.L., Harris S.E., Youseff B.H., Presloid J.B., Burlak C., Munshi-South J., Best S.M, & Taylor R.T. (2017). Interferon signaling in Peromyscus leucopus confers a potent and specific restriction to vector-borne flaviviruses. PLoS ONE, 12(6), e0179781.
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3

Immunoblotting analysis of PRC2 inhibitors

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Antibodies used for immunoblotting analysis in this study include: αEZH2 (BD Biosciences, #612667), αActin (Sigma-Aldrich, #A5441), αH3K27me3 (Cell Signaling, #9733), αH3 (Cell Signaling, #9715), αpSTAT2 (Cell Signaling, #88410), αSTAT2 (Santa Cruz, #sc-1668 and Cell Signaling, #72604), αpSTAT1 (Cell Signaling, #9167), αSTAT1 (Cell Signaling, #9172) and αGAPDH (Santa Cruz, #sc-365062). Antibodies that were applied in other assays, such as ChIP, fluorescent immunohistochemistry and flow cytometry, are specified in the corresponding sections.
In this study, the following inhibitors of the PRC2 activity were included: GSK503 (Selleckchem, #S7804), GSK126 (Xcessbio, #60071), EPZ-6438 (Xcessbio, #60122) and EED226 (Selleckchem, #S8496). The interferon (IFN) ligands used in this study are: IFNβ, IFNγ and IL-29, and their details are provided in the section “Neutralization of IFN ligands” below.
Hong J., Lee J.H., Zhang Z., Wu Y., Yang M., Liao Y., de la Rosa R., Scheirer J., Pechacek D., Zhang N., Xu Z., Curiel T., Tan X., Huang T.H, & Xu K. (2022). PRC2-mediated epigenetic suppression of type I IFN-STAT2 signaling impairs antitumor immunity in luminal breast cancer. Cancer research, 82(24), 4624-4640.
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4

Characterizing Immune Signaling Pathways

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Gp96, calreticulin, and HSP90 were isolated from murine livers as previously described10 (link),22 (link). The quality and purity of the gp96 preparations was vigilantly verified by SDS-PAGE gel and immunoblotting and is consistent with our previous studies.
For immunoblots, cells were lysed with NP-40 and lysates were run on SDS-PAGE gels and transferred to nitrocellulose membranes. Membranes were probed with the following antibodies α -STAT1(14944), α -pSTAT1 S727(9177), α -pSTAT1 Y701(9167), α -pp38 MAPK(9167), α -p38 MAPK(9216), α - β -actin(8457), (Cell Signaling) and α -SHP2(sc-7384) (Santa Cruz). Immunoblots comparing different cell types and timepoints were run with all on a singular membrane when possible. Membranes were developed using a Proteinsimple imager and quantitated using Alpha View software.
Luminex (eBioscience) consisted of the single analytes: IL-12, IL-18, IL-15/IL-15R, IFN- α , IP-10, and RANTES. Samples were analyzed using the manufacturer’s protocol and a Bioplex II Luminex machine.
CXCL10 and CCL5 ELISAs were purchased from eBioscience and samples were analyzed following the manufacturer’s protocol.
Sedlacek A.L., Kinner-Bibeau L.B., Wang Y., Mizes A.P, & Binder R.J. (2021). Tunable heat shock protein-mediated NK cell responses are orchestrated by STAT1 in Antigen Presenting Cells. Scientific Reports, 11, 16106.
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