For locomotor activity and ex vivo neurochemistry studies, a total number of 152 male NMRI mice weighing 18–25 g at arrival were supplied by Janvier (France). For in vivo microdialysis, a total number of 54 male Wistar rats weighing 260–280 g at arrival were supplied by Envigo (Netherlands). Mice were housed eight to a cage whereas rats were housed four to a cage prior to surgery and single-housed after surgery, under controlled environmental conditions comprising constant room temperature of 20–22 °C, humidity of 50–65% and regular light-dark conditions with lights on at 07:00 a.m. and off at 07:00 p.m. Before any experimental procedures were initiated, animals were allowed at least one week of acclimatization to the facilities. All animals had access to regular chow (Harlan Teklad, England) and tap water ad libitum. The experiments were approved by the Ethics Committee for Animal Experiments, Gothenburg, Sweden.
Nmri mice
Manufactured by Janvier Labs
Sourced in France
NMRI mice are a widely used strain of laboratory mice. They are genetically homogeneous and have a natural tendency to develop spontaneous tumors, making them a valuable model for cancer research. NMRI mice are characterized by their high fertility and adaptability to various experimental conditions.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Regular chow, by Inotiv (1 mentions)
R m h, by Ssniff (1 mentions)
Female c57bl 6 mice, by Janvier Labs (1 mentions)
Sas v9, by SAS Institute (1 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)
Muriplan software, by Xstrahl (1 mentions)
Sarrp, by Xstrahl (1 mentions)
Wt mice, by Charles River Laboratories (1 mentions)
Matrigel, by BD (1 mentions)
Heat inactivated, by Thermo Fisher Scientific (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
30 protocols using nmri mice
1
Rodent Acclimation and Behavioral Studies
2
Mouse Housing and Acclimatization Protocols
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Female outbred NMRI mice (Janvier Labs, Saint Berthevin Cedex, France) were housed in an individually ventilated cage system (Ebeco, Castrop-Rauxel, Germany) under pathogen-free conditions with water and standard food (R/M-H, ssniff Spezialdiäten GmbH, Soest, Germany) given ad libitum. After acclimatization for at least one week, studies were performed according to the guidelines for the Care and Use of Laboratory Animals and the study was approved by the Animal Care and Usage Committee of the state agency Leipzig (Landesdirektion Leipzig, file numbers 24-9168.11/14/37 and 24-9168.11/17/43).
Female outbred CD-1 mice (Innovo, Gödöllő, Hungary) were acclimatized 1 week after arrival in a sterile plastic type 2 cage (Innovo Kft., Gödöllő) on softwood granules with free access to water and sterile pelleted rodent food (Szinbád Ltd., Gödöllő). All animals were cared for and experiments were performed in accordance with the recommendations of the Guidelines for the Care and Use of Laboratory Animals and the study was approved by the Animal Care Committees of Semmelweis University (permission No.: 001/2218-4/2012).
Female outbred CD-1 mice (Innovo, Gödöllő, Hungary) were acclimatized 1 week after arrival in a sterile plastic type 2 cage (Innovo Kft., Gödöllő) on softwood granules with free access to water and sterile pelleted rodent food (Szinbád Ltd., Gödöllő). All animals were cared for and experiments were performed in accordance with the recommendations of the Guidelines for the Care and Use of Laboratory Animals and the study was approved by the Animal Care Committees of Semmelweis University (permission No.: 001/2218-4/2012).
3
Visualizing Myonuclei with ACTA1-Driven mCherry
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To visualize myonuclei by an independent method a custom AAV6 vector comprised of the human Actin, Alpha-1 (ACTA1) gene promoter and nuclear mCherry was constructed. The promoter region (3111 bp to 740 bp upstream of the transcription start site) for the ACTA1 gene was amplified by PCR and sub cloned into the pCherry vector carrying a nuclear localization signal (NLS) upstream of the mCherry cassette. The expression cassette was then cloned into the pAAV6-Basic vector. The final construct was validated by Sanger sequencing (results not shown). Virus carrying the ACTA1-Vector was produced by Vector Biolabs. For transduction, 6-week-old female NMRI mice (Janvier Labs, France) was injected with 5x10^9 genome copies of Adeno-Associated Virus 6 in 20 μl sterile Dulbecco's phosphatebuffered saline (DPBS). 14 days after transduction Tibialis anterior (TA) muscles were isolated as described below.
4
Angiogenesis assay in mice
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PBS (n = 5) or VEGF222/NF (n = 5) (200 ng) was embedded in Matrigel® growth factor reduced and injected subcutaneously into the right and left flank of female NMRI mice (Janvier Labs, 2 plugs per mouse). Plugs were removed after 2 weeks for quantification of the haemoglobin.
5
Secretin and BI-3434 Effects on Mouse Food Intake
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Experimental protocols concerning the use of laboratory animals were reviewed by a federal Ethics Committee and approved by governmental authorities. NMRI mice were obtained from Janvier Labs, France and were group housed 4 mice per cage on a 12/12 dark/light cycle (lights off 3 pm). Room temperature was set to 21 °C ± 1 °C, with 60% ± 20% humidity. Animals had ad libitum access to regular rodent chow (Kliba NAFAG 3430, Granovit AG, 4303 Kaiseraugst, Switzerland) and tap water. 6-week-old male mice were randomized to treatment groups under the constraint of similar average body weight and food intake in all groups (n = 8–10/group). 6 h before the start of the dark cycle animals were fasted. Stapled secretin or BI-3434 (both 30, 100 and 300 nmol/kg, s.c.) were applied 1 h before the dark cycle. Food intake was measured for 24 h using a fully automated HerdsMan-2 food intake monitoring system (MBRose, Denmark). Blood was collected 24 h post dosing for exposure measurements. One week later, BI-3434 was injected again and blood was collected at Cmax (2 h) for biomarker analysis.
6
Outbred NMRI Mouse Experiments
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All animal experiments were performed according to European regulations concerning FELASA category B and GVSOLAS standard guidelines. Animal experiments were approved by German authorities (Regierungspraesidium Karlsruhe, Germany), § 8 Abs. 1 Tierschutzgesetz (TierSchG) under the license G-260/12 and were performed according to National and European regulations. Two female outbred NMRI mice (8- to 10-week-old) purchased from Janvier laboratories, France, were used. Mice were kept in groups of 2 to 4 mice per cage under specified pathogen-free (SPF) conditions within the animal facility at Heidelberg University (IBF) on a 12-hour light/dark cycle at 22°C (± 2°C) with
ad libitum access to food and water.
ad libitum access to food and water.
7
Postnatal Developmental Milestones in NMRI Mice
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National Marine Research Institute (NMRI) mice, purchased from Janvier Lab (France) were housed at 22 °C with food and water ad libitum and with a 12 h light/dark cycle (lights on from 7 a.m. to 7 p.m.). Male and female mice are handled every day from P5 to P45. Males and females are weaned and put in separate cages at P21. Animal handling is performed according to the recommendations of the European Communities Council directives (2010/63/UE) and the French national legislation, with an authorization delivered by the French Ministry of Education and Research (agreement no. 01680.02/2014, October 13, 2014; APAFIS#221362019092013438607 v4 from Ministère de l’Agriculture et de la Pêche). At P6, P10 and P45, mice are euthanized respecting ethical methods (P6 and P10: decapitation after profound anesthesia with isoflurane 5%; P45: cervical elongation under profound anesthesia with isoflurane 5%, VETFLURANE®). The number of animals is minimized as much as possible. Animal welfare is insured throughout the study.
8
Anesthetized NMRI Mice for Imaging
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Ten-week-old male NMRI mice (37–49 g) (Elevage Janvier, Le Genest Saint Isle, France) were housed under standard conditions with food and water ad libitum. All procedures were approved by the regional animal committee and were in accordance with international guidelines on the ethical use of animals. To reduce tissue auto-fluorescence 7 days prior and during the whole experiment, mice were fed with a special diet consisting of food low in manganese (C 1039 Altromin, Lage, Germany). During the imaging experiments mice were anesthetized using oxygen with 2% isoflurane (Actavis, Germany).
9
Tumor Induction and Imaging in Mice
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Transplantable liver tumors (TLT hepatocarcinoma Taper et al., 1966 (link)) were induced i.m. into the right gastrocnemius muscle of 5-week-old male NMRI mice (Janvier, France). Tumors were allowed to reach up to 8 ± 0.5 mm in diameter prior to experimentation. For all experiments, mice were anesthetized using isoflurane (3% for induction, 1.5% for maintenance, mixed with air). Body temperature was maintained at 37.0 ± 1.0°C with a circulating water blanket and monitored together with respiration rate during experiments. All animal experiments were performed in accordance to national animal care regulations with the approval of local Ethics Board 2010/UCL/MD/01. CA4 (Sigma-Aldrich, Belgium) dissolved in DMSO was delivered i.p. at a dose of 100 mg/kg (Grosios et al., 1999 (link)).
19F NMR and DCE-MRI experiments were performed on separate cohorts of mice, because of the possible influence of the contrast agent on fluorine relaxation times (Ratner et al., 1989 (link)).
19F NMR and DCE-MRI experiments were performed on separate cohorts of mice, because of the possible influence of the contrast agent on fluorine relaxation times (Ratner et al., 1989 (link)).
10
Murine Xenograft Model of Oral Squamous Cell Carcinoma
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CAL27 cells (2 × 106) or SCC154 cells (7.5 × 106) were subcutaneously injected in each flank of 6–7-week-old female nu/nu Naval Medical Research Institute (NMRI) mice (Janvier Labs, France). The mice were either treated with vehicle or with AZD6738 (50 mg/kg body weight), which were all administered via gavage. AZD6738 was delivered 5 days before RT treatment and 2 h before fractionated RT (2Gy/fraction) with a total dose of 10Gy. Tumor volumes were determined with caliper measurements and calculated as V = π/6x_1xd_2xd_3. The bodyweight and health of the mice were monitored daily during treatment and three times per week during follow-up. The experiment was performed according to the Ethical committee Animal Experimentation of KU Leuven (P163/2017; approval date 29 September 2017).
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