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Mouse trap

Manufactured by Immunodiagnostic Systems
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The Mouse TRAP is a laboratory equipment designed to measure the activity of the Thyroid Stimulating Hormone Receptor (TSHR) in mouse samples. It provides a quantitative assessment of TSHR-mediated cellular responses.

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Lab products found in correlation

Oc assay kits, by Biomedical Technologies (1 mentions) Mouse cytokine antibody array, by R&D Systems (1 mentions) Quantikine elisa kit, by R&D Systems (1 mentions) Ab108821, by Abcam (1 mentions) Synergy ht, by Agilent Technologies (1 mentions) Ac 33f1, by Immunodiagnostic Systems (1 mentions) Mouse rat pinp, by Immunodiagnostic Systems (1 mentions)

4 protocols using mouse trap

1

Serum Biomarker Quantification Protocol

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Sterile blood was collected at the time of harvest, allowed to clot at room temperature for 5 minutes and then centrifuged at 5000g for 10 minutes. Serum was removed, aliquoted and snap frozen and in liquid nitrogen, and stored at −80°C. Serum tartrate resistant acid phosphatase 5b (TRAP5b) and osteocalcin (OC) were measured using mouse TRAP (Immunodiagnostic Systems Inc., Fountain Hills, AZ) and OC assay kits (Biomedical Technologies Inc., Stoughton, MA), respectively, according to manufacturer’s protocol.
Schepper J.D., Collins F., Rios-Arce N.D., Raehtz S., Schaefer L., Gardinier J.D., Britton R., Parameswaran N, & McCabe L.R. (2019). Probiotic Lactobacillus reuteri prevents post-antibiotic bone loss by reducing intestinal dysbiosis and preventing barrier disruption. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 34(4), 681-698.
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2

Measuring Inflammatory Cytokines and Bone Resorption Markers

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We determined serum IL-4 and IL-13 levels using the Mouse Cytokine Antibody Array (R&D Systems, Inc., Minneapolis, MN, USA) in duplicate; we quantified the density using Image J software and normalized it to that of the positive control. We determined serum resorption marker TRAP5b (TRAP) with MouseTRAP (TRAcP 5b) ELISA (Catalogue number: SB-TR103, Immuno Diagnosticsystems, Gaithersburg, MD). TNF and IL-1β levels were determined with Quantikine ELISA Kits from R&D Systems (Catalogue number: MTA00B and MLB00C) following the instructions from the manufacturers.
Guo Y., Xie C., Li X., Yang J., Yu T., Zhang R., Zhang T., Saxena D., Snyder M., Wu Y, & Li X. (2017). Succinate and its G-protein-coupled receptor stimulates osteoclastogenesis. Nature Communications, 8, 15621.
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3

Serum Biomarkers of Bone Metabolism

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Serum samples were obtained by cardiac puncture at sacrifice followed by centrifugation in serum separator tubes (BD Microtainer #365967, Franklin Lakes, NJ) and stored at −80°C until analysis. A mouse TRAcP-5b ELISA (MouseTrap™; #SB-TR103, Immunodiagnostic Systems, Gaithersburg, MD) was used to measure the circulating bone resorption marker tartrate-resistant acid phosphatase 5b. Additional aliquots of serum were analyzed in a rat/mouse procollagen I intact N-terminal (P1NP) enzyme immunoassay (#AC-33F1, Immunodiagnostic Systems, Gaithersburg, MD) for quantification of bone formation markers. Serum glucocorticoids were measured using a corticosterone ELISA (#ab108821, Abcam, Cambridge, MA) using a 100-fold dilution of mouse serum and provided protocols. All ELISA plates were measured with a microplate reader (BioTek Synergy HT, Winooski, VT) using Gen5 software and readings at 405 (P1NP) and 450 nm (TRAcP-5b, Corticosterone) wavelengths, as recommended for each assay. Serum data were normalized to calibrants and standards provided in respective ELISA kits.
Pierce J.L., Ding K.H., Xu J., Sharma A.K., Yu K., del Mazo Arbona N., Rodríguez-Santos Z., Bernard P.J., Bollag W.B., Johnson M.H., Hamrick M.W., Begun D.L., Shi X.M., Isales C.M, & McGee-Lawrence M.E. (2019). The glucocorticoid receptor in osteoprogenitors regulates bone mass and marrow fat. The Journal of endocrinology.
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4

Quantification of Bone Markers PINP and TRAcP5b

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Serum levels of the bone formation marker procollagen type I N-terminal propeptide (PINP; Mouse/Rat PINP, Immunodiagnostic Systems) and the bone resorption marker tartrate-resistant acid phosphatase 5b (TRAcP5b, Mouse TRAP, Immunodiagnostic Systems) were quantified by enzyme immunoassays. Serum tests were performed in duplicate.
Marulanda J., Tauer J.T., Boraschi-Diaz I., Bardai G, & Rauch F. (2023). Effect of sclerostin inactivation in a mouse model of severe dominant osteogenesis imperfecta. Scientific Reports, 13, 5010.
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