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Cd45 pe dazzle5 clone 30 f11

Manufactured by BioLegend
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CD45-PE-Dazzle594 clone 30-F11 is a fluorochrome-conjugated antibody that binds to the CD45 antigen. CD45 is a transmembrane glycoprotein tyrosine phosphatase expressed on the surface of most hematopoietic cells. This product can be used in flow cytometry applications.

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Lab products found in correlation

Cd45 pe cy7 clone 30 f11, by BioLegend (1 mentions) Cd14 percp cy5.5 clone hcd14, by BD (1 mentions) Cd8 fitc clone rpa t8, by BD (1 mentions) Cd45ra pecy7 clone hi 100, by BD (1 mentions)

Spelling variants of this product

CD45-PE (78 citations) CD45 (30-F11, (72 citations) CD45 (clone 30-F11, (62 citations) Clone 30-F11 (48 citations) CD45 PE clone 30-F11 (3 citations)

2 protocols using cd45 pe dazzle5 clone 30 f11

1

Multicolor Flow Cytometry Immunophenotyping

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From BioLegend (San Diego, CA, USA): CD45-PE-Cy7 clone 30-F11, CD45-PE-Dazzle5 clone 30-F11. The following anti-human antibodies were used for flow cytometry: from BD Biosciences (San Jose, CA, USA): CD45-V500 clone H130, CD34-FITC clone 581, CD8-FITC clone G42-8, CD11c-allophycocyanin clone B-ly6; from BioLegend: CD56-allophycocyanin-Cy7 clone HCD56, HLA-DR-BV510 clone L243, CD3-BV605 clone UCHT1, CD20-BV650 clone 2H7, CD16-BV117 clone B73.1, CD45-BV785 clone H130, CD8-FITC clone RPA-T8, CD33-PE clone WM53, CD14-PerCP-Cy5.5 clone HCD14, CD45RA-PE-Cy7 clone HI100, CD56-allophycocyanin clone QA17A16, CD4-Alexa Fluor 700 clone SK3; from Thermo Scientific: CD14-Alexa 700 clone Tuk4, CD3-PE-Cy5.5 clone 7D6, CD4-PE clone RPA-T4, CD19-PacBlue clone SJ25C1, CD16-PE-TexasRed clone 3G8.
Kenney D.J., O’Connell A.K., Turcinovic J., Montanaro P., Hekman R.M., Tamura T., Berneshawi A.R., Cafiero T.R., Al Abdullatif S., Blum B., Goldstein S.I., Heller B.L., Gertje H.P., Bullitt E., Trachtenberg A.J., Chavez E., Nono E.T., Morrison C., Tseng A.E., Sheikh A., Kurnick S., Grosz K., Bosmann M., Ericsson M., Huber B.R., Saeed M., Balazs A.B., Francis K.P., Klose A., Paragas N., Campbell J.D., Connor J.H., Emili A., Crossland N.A., Ploss A, & Douam F. (2022). Humanized mice reveal a macrophage-enriched gene signature defining human lung tissue protection during SARS-CoV-2 infection. Cell Reports, 39(3), 110714.
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2

Comprehensive Cytometry Antibody Panel

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The anti-mouse antibody CD45-PE-Dazzle5 clone 30-F11 was purchased from BioLegend (San Diego, CA, USA) and used for flow cytometry. The following anti-human antibodies were used for flow cytometry and were all acquired from BD Biosciences (San Jose, CA, USA): HLA-DR-BV510 clone L243, CD3-BV605 clone UCHT1, CD20-BV650 clone 2H7, CD16 (FcγRIII)-BV117 clone B73.1, CD45-BV785 clone H130, CD8-FITC clone RPA-T8, CD33-PE clone WM53, CD14-PerCP-Cy5.5 clone HCD14, CD45RA-PE-Cy7 clone HI100, CD56-allophycocyanin clone QA17A16, CD4-Alexa Fluor 700 clone SK3. The following anti-human antibodies were used for in vivo depletions: From Bio-X Cell (Lebanon, NH, USA): anti-human CD3 clone OKT3, anti-human CD4 clone OKT4, anti-human CD8 clone OKT8; From Thermofisher: mouse IgG2a isotype. The anti-SARS-CoV-2 (2019-nCoV) Spike RBD-mFc clone #57 used as a control for neutralization assays was acquired from Sino Biologicals.
Kenney D., O’Connell A.K., Tseng A.E., Turcinovic J., Sheehan M.L., Nitido A.D., Montanaro P., Gertje H.P., Ericsson M., Connor J.H., Vrbanac V., Crossland N.A., Harly C., Balazs A.B, & Douam F. (2024). Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytes. bioRxiv.
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