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Coolcell freezing container

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CoolCell freezing containers are designed to provide controlled and uniform cooling of biological samples during cryogenic storage. The containers utilize a specialized cooling pattern to ensure consistent freezing rates, protecting the integrity of the samples.

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Lab products found in correlation

Recovery cell culture freezing medium, by Thermo Fisher Scientific (4 mentions) Edta tubes, by Avantor (4 mentions) Tc20 automated cell counter, by Bio-Rad (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by AppliChem (1 mentions) Fbs superior, by Merck Group (1 mentions) Trypsin, by PAN Biotech (1 mentions) Accumax, by Thermo Fisher Scientific (1 mentions) Profreeze freezing medium, by Lonza (1 mentions) Dispase, by STEMCELL (1 mentions) Cryostor cs10 solution, by STEMCELL (1 mentions) Glycerol, by Merck Group (1 mentions) Cryotube, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions) Cts knockout sr xenofree medium, by Thermo Fisher Scientific (1 mentions) Nutristem hpsc xf medium, by Sartorius (1 mentions)

Spelling variants of this product

CoolCell (14 citations) CoolCell Containers (10 citations) CoolCell™ LX Cell Freezing Container (4 citations) Freezing container (2 citations)

9 protocols using coolcell freezing container

1

Cartilage Tissue Expansion and Cryopreservation

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The cartilage samples were cut into pieces of <1 mm3 with a scalpel. After transfer of 25 mg/cm2 to 6-well tissue culture plates, the samples were cultured in expansion medium (EM) consisting of low glucose DMEM (PAN Biotech) supplemented with 10 % fetal bovine serum (FBS Superior, Sigma-Aldrich), 100 U/ml penicillin, 100 μg/ml streptomycin (Thermo Fisher) and 2 mM L-alanyl-l-glutamine (GlutaMAX, Thermo Fisher). The first media change was performed at day four (d4) after seeding. Subsequent media changes were performed twice a week. At day 14–16 of primary cell culture, the cells reached 80 % confluence, were detached with trypsin (PAN Biotech), counted using the TC20 automated cell counter (Bio-Rad) and re-seeded at a cell density of 1.000 cells/cm2. 1 × 106 cells were cryopreserved at cell culture passage two in 500 μl freezing media consisting of low glucose DMEM supplemented with 12.5 % human serum albumin (Albiomin, Biotest AG) and 10 % Dimethyl sulfoxide (DMSO, Applichem). The cell suspension was stored at −80 °C in CoolCell™ freezing containers (Corning) to ensure a repeatable −1 °C/min cooling rate before storing the cells in liquid nitrogen.
Zimmerer A., Schulze F., Gebhardt S., Huesker K., Stobbe D., Grolimund D., Hesse B., Wassilew G.I, & Schoon J. (2024). Impact of gadolinium-based MRI contrast agent and local anesthetics co-administration on chondrogenic gadolinium uptake and cytotoxicity. Heliyon, 10(8), e29719.
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2

Isolation of PBMCs and Plasma from Whole Blood

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Whole blood was collected in EDTA tubes (VWR) and stored at 4 °C until processing. All samples were processed within 24 h. Time of blood draw, processing and freezing was recorded for each sample. Before processing, tubes were brought to room temperature. Peripheral blood mononuclear cells (PBMCs) and plasma were isolated by density gradient centrifugation using pre-filled centrifugation tubes (pluriSelect). Up to 30 ml of undiluted blood was added on top of the sponge and centrifuged for 30 min at 1,000g at room temperature. Plasma was carefully removed and then centrifuged for 10 min at 4,000g to remove debris, aliquoted and stored at −80 °C. The cell layer was then collected and washed twice in PBS by centrifugation for 10 min at 300g at room temperature. PBMCs were resuspended in Recovery Cell Culture Freezing Medium (Thermo Fisher Scientific) containing 10% DMSO, placed overnight in CoolCell freezing containers (Corning) at −80 °C and then stored in liquid nitrogen.
Au L., Fendler A., Shepherd S.T., Rzeniewicz K., Cerrone M., Byrne F., Carlyle E., Edmonds K., Del Rosario L., Shon J., Haynes W.A., Ward B., Shum B., Gordon W., Gerard C.L., Xie W., Joharatnam-Hogan N., Young K., Pickering L., Furness A.J., Larkin J., Harvey R., Kassiotis G., Gandhi S., Swanton C., Fribbens C., Wilkinson K.A., Wilkinson R.J., Lau D.K., Banerjee S., Starling N., Chau I, & Turajlic S. (2021). Cytokine release syndrome in a patient with colorectal cancer after vaccination with BNT162b2. Nature Medicine, 27(8), 1362-1366.
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3

Isolation of PBMC and Plasma from Whole Blood

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Whole blood was collected in EDTA tubes (VWR) and stored at 4°C until processing. All samples were processed within 24 hours. Time of blood draw, processing, and freezing was recorded for each sample. Prior to processing tubes were brought to room temperature (RT). PBMC and plasma were isolated by density-gradient centrifugation using pre-filled centrifugation tubes (pluriSelect). Up to 30 ml of undiluted blood was added on top of the sponge and centrifuged for 30 minutes at 1000g at RT. Plasma was carefully removed then centrifuged for 10 minutes at 4000g to remove debris, aliquoted and stored at -80°C. The cell layer was then collected and washed twice in PBS by centrifugation for 10 minutes at 300g at RT. PBMC were resuspended in Recovery cell culture freezing medium (Fisher Scientific) containing 10% DMSO, placed overnight in CoolCell freezing containers (Corning) at -80°C and then stored at -80°C.
Fendler A., Shepherd S.T., Au L., Wilkinson K.A., Wu M., Byrne F., Cerrone M., Schmitt A.M., Joharatnam-Hogan N., Shum B., Tippu Z., Rzeniewicz K., Boos L.A., Harvey R., Carlyle E., Edmonds K., Del Rosario L., Sarker S., Lingard K., Mangwende M., Holt L., Ahmod H., Korteweg J., Foley T., Bazin J., Gordon W., Barber T., Emslie-Henry A., Xie W., Gerard C.L., Deng D., Wall E.C., Agua-Doce A., Namjou S., Caidan S., Gavrielides M., MacRae J.I., Kelly G., Peat K., Kelly D., Murra A., Kelly K., O’Flaherty M., Dowdie L., Ash N., Gronthoud F., Shea R.L., Gardner G., Murray D., Kinnaird F., Cui W., Pascual J., Rodney S., Mencel J., Curtis O., Stephenson C., Robinson A., Oza B., Farag S., Leslie I., Rogiers A., Iyengar S., Ethell M., Messiou C., Cunningham D., Chau I., Starling N., Turner N., Welsh L., van As N., Jones R.L., Droney J., Banerjee S., Tatham K.C., O’Brien M., Harrington K., Bhide S., Okines A., Reid A., Young K., Furness A.J., Pickering L., Swanton C., Gandhi S., Gamblin S., Bauer D.L., Kassiotis G., Kumar S., Yousaf N., Jhanji S., Nicholson E., Howell M., Walker S., Wilkinson R.J., Larkin J, & Turajlic S. (2021). Adaptive immunity and neutralizing antibodies against SARS-CoV-2 variants of concern following vaccination in patients with cancer: The CAPTURE study. Nature cancer, 2, 1321-1337.
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4

Isolation of PBMC and Plasma from Whole Blood

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Whole blood was collected in EDTA tubes (VWR) and stored at 4°C until processing. All samples were processed within 24 hours. Time of blood draw, processing, and freezing was recorded for each sample. Prior to processing tubes were brought to room temperature (RT). PBMC and plasma were isolated by density-gradient centrifugation using pre-filled centrifugation tubes (pluriSelect). Up to 30 ml of undiluted blood was added on top of the sponge and centrifuged for 30 minutes at 1000g at RT. Plasma was carefully removed then centrifuged for 10 minutes at 4000g to remove debris, aliquoted and stored at -80°C. The cell layer was then collected and washed twice in PBS by centrifugation for 10 minutes at 300g at RT. PBMC were resuspended in Recovery cell culture freezing medium (Fisher Scientific) containing 10% DMSO, placed overnight in CoolCell freezing containers (Corning) at -80°C and then stored at -80°C.
Fendler A., Shepherd S.T., Au L., Wilkinson K.A., Wu M., Byrne F., Cerrone M., Schmitt A.M., Joharatnam-Hogan N., Shum B., Tippu Z., Rzeniewicz K., Boos L.A., Harvey R., Carlyle E., Edmonds K., Del Rosario L., Sarker S., Lingard K., Mangwende M., Holt L., Ahmod H., Korteweg J., Foley T., Bazin J., Gordon W., Barber T., Emslie-Henry A., Xie W., Gerard C.L., Deng D., Wall E.C., Agua-Doce A., Namjou S., Caidan S., Gavrielides M., MacRae J.I., Kelly G., Peat K., Kelly D., Murra A., Kelly K., O’Flaherty M., Dowdie L., Ash N., Gronthoud F., Shea R.L., Gardner G., Murray D., Kinnaird F., Cui W., Pascual J., Rodney S., Mencel J., Curtis O., Stephenson C., Robinson A., Oza B., Farag S., Leslie I., Rogiers A., Iyengar S., Ethell M., Messiou C., Cunningham D., Chau I., Starling N., Turner N., Welsh L., van As N., Jones R.L., Droney J., Banerjee S., Tatham K.C., O’Brien M., Harrington K., Bhide S., Okines A., Reid A., Young K., Furness A.J., Pickering L., Swanton C., Gandhi S., Gamblin S., Bauer D.L., Kassiotis G., Kumar S., Yousaf N., Jhanji S., Nicholson E., Howell M., Walker S., Wilkinson R.J., Larkin J, & Turajlic S. (2021). Adaptive immunity and neutralizing antibodies against SARS-CoV-2 variants of concern following vaccination in patients with cancer: The CAPTURE study. Nature cancer, 2, 1321-1337.
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5

Bovine Trophoblast Stem Cell Culture

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Bovine male TSCs were derived and cultured in LCDM media (CELL-REPORTS-D-23-01022) with slight modifications (N2B27 base, 1% BSA, 10ng/ml LIF, 3 μM Chir99021, 2μM Minocycline hydrochloride (M), 2μM (S)-(+)-Dimethindene maleate (D). During passaging cells were treated with Accumax (Thermo Fisher) or Dispase (STEMCELL Technologies) for 5 minutes at 37°C (no PBS wash), cells were collected with the same volume of bTSC medium and gently lifted of the plate using a wide opening p100 pipette tip and gentle force. Cells were split at a 1:3 ratio and plated on iMEFs with CEPT. Only 1ml of media was plated in a 6 well for the first 24h to facilitate bTSCs attachment. bTSCs do not survive well after single-cell dissociation and tend to form trophospheres if not plated correctly. These steps are critical for long term culture and expansion of bTSCs. Cells were cryopreserved in CoolCell freezing containers (Corning) in 45 % LCDM 45% FBS and 10% DMSO or ProFreeze Freezing medium (Lonza, 12–769E) at 2×10^6 cells per ml.
Pinzón-Arteaga C.A., Wang Y., Wei Y., Ribeiro Orsi A.E., Li L., Scatolin G., Liu L., Sakurai M., Ye J., Ming H., Yu L., Li B., Jiang Z, & Wu J. (2023). Bovine blastocyst-like structures derived from stem cell cultures. Cell stem cell, 30(5), 611-616.e7.
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6

Standardized PBMC Isolation from Whole Blood

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Whole blood was collected in EDTA tubes (VWR) and stored at 4°C until processing. All samples were processed within 24 hours. Time of blood draw, processing, and freezing was recorded for each sample. Prior to processing tubes were brought to room temperature (RT). PBMC and plasma were isolated by density-gradient centrifugation using pre-filled centrifugation tubes (pluriSelect). Up to 30 ml of undiluted blood was added on top of the sponge and centrifuged for 30 minutes at 1000g at RT. Plasma was carefully removed then centrifuged for 10 minutes at 4000g to remove debris, aliquoted and stored at −80°C. The cell layer was then collected and washed twice in PBS by centrifugation for 10 minutes at 300 x g at RT. PBMC were resuspended in Recovery cell culture freezing medium (Fisher Scientific) containing 10% DMSO, placed overnight in CoolCell freezing containers (Corning) at −80°C and then stored at −80°C.
Fendler A., Au L., Shepherd S.T., Byrne F., Cerrone M., Boos L.A., Rzeniewicz K., Gordon W., Shum B., Gerard C.L., Ward B., Xie W., Schmitt A.M., Joharatnam-Hogan N., Cornish G.H., Pule M., Mekkaoui L., Ng K.W., Carlyle E., Edmonds K., Del Rosario L., Sarker S., Lingard K., Mangwende M., Holt L., Ahmod H., Stone R., Gomes C., Flynn H.R., Agua-Doce A., Hobson P., Caidan S., Howell M., Wu M., Goldstone R., Crawford M., Cubitt L., Patel H., Gavrielides M., Nye E., Snijders A.P., MacRae J.I., Nicod J., Gronthoud F., Shea R.L., Messiou C., Cunningham D., Chau I., Starling N., Turner N., Welsh L., van As N., Jones R.L., Droney J., Banerjee S., Tatham K.C., Jhanji S., O’Brien M., Curtis O., Harrington K., Bhide S., Bazin J., Robinson A., Stephenson C., Slattery T., Khan Y., Tippu Z., Leslie I., Gennatas S., Okines A., Reid A., Young K., Furness A.J., Pickering L., Gandhi S., Gamblin S., Swanton C., Nicholson E., Kumar S., Yousaf N., Wilkinson K.A., Swerdlow A., Harvey R., Kassiotis G., Larkin J., Wilkinson R.J, & Turajlic S. (2021). Functional antibody and T-cell immunity following SARS-CoV-2 infection, including by variants of concern, in patients with cancer: the CAPTURE study. Research Square.
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7

Cardiovascular Tissue and Blood Sampling

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Cardiac tissue and blood samples were collected in the operating room during various paediatric cardiovascular surgeries. Cardiac tissue samples were kept in cold saline on ice during transfer to the laboratory for preservation. Blood samples were collected before cardiac bypass was initiated into EDTA-coated vacutainers and were then transferred to the laboratory on ice. Cardiac tissue samples were carefully dissected into multiple aliquots, some of which were flash-frozen and stored at −80 °C and others of which were fixed in 10% neutral buffered formalin for 16 to 24 h at 4 °C. Formalin-fixed samples then underwent serial dehydration and were embedded in paraffin blocks for histology. Formalin-fixed paraffin-embedded (FFPE) samples were then used to make a tissue microarray (2 mm cores) for high-throughput image analysis. After the isolation of PBMCs, cells to be cryopreserved were resuspended in CryoStor CS10 solution (StemCell Technologies, catalogue (cat.) no. 07930) and frozen at a controlled rate by using a Corning CoolCell Freezing Container. Isolated plasma was stored at −80 °C.
Hill M.C., Kadow Z.A., Long H., Morikawa Y., Martin T.J., Birks E.J., Campbell K.S., Nerbonne J., Lavine K., Wadhwa L., Wang J., Turaga D., Adachi I, & Martin J.F. (2022). Integrated multi-omic characterization of congenital heart disease. Nature, 608(7921), 181-191.
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8

Cryogenic Preservation and Thawing of Cellular Capsules

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Before freezing, capsules were loaded using the same procedure, but with cells resuspended in freezing medium (GM supplemented with 10% glycerol [Sigma Aldrich, 49,767]) and immediately placed in silicone tubes prefilled with freezing medium, after which they were transferred to cryotubes. The cryotubes were quickly transferred to a CoolCell freezing container (Corning) and frozen at −80°C overnight. For long-term storage, cryotubes were conserved in liquid nitrogen.
The thawing process involved rapidly transferring the frozen capsules in silicone tubes from liquid nitrogen to pre-warmed GM in a 10-cm Petri dish. After gentle agitation, capsules were removed from the silicone tubes and placed in 12-well plates containing 1 mL GM.
Lathuiliere A., Vernet R., Charrier E., Urwyler M., Von Rohr O., Belkouch M.C., Saingier V., Bouvarel T., Guillarme D., Engel A., Salmon P., Laumonier T., Grogg J, & Mach N. (2022). Immortalized human myoblast cell lines for the delivery of therapeutic proteins using encapsulated cell technology. Molecular Therapy. Methods & Clinical Development, 26, 441-458.
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9

Cryopreservation of Stem Cells

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The cells were rinsed with phosphate-buffered saline (PBS, Gibco), non-enzymatically passaged by 0.5 mM of EDTA (Invitrogen), and spined for 4 min/300 g. Cells (0.5 × 106/mL) were resuspended in cold freezing medium consisting of 65% NutriStem® hPSC XF Medium (Biological Industries), 25% CTS Knockout SR XenoFree Medium (Gibco), and 10% CryoSure-DMSO (WAK chemie) supplemented with 10 µM ROCK inhibitor and frozen in cryotubes (Nunc) placed in Corning CoolCell Freezing container at the rate of -1 °C/minute in −80°C freezer. cryotubes were moved to nitrogen vapors (− 196 °C) after 24 h.
Souralova T., Rehakova D., Jeseta M., Tesarova L., Beranek J., Ventruba P., Hampl A, & Koutna I. (2022). The Manufacture of Xeno- and Feeder-Free Clinical-Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy. International Journal of Molecular Sciences, 23(20), 12500.
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