2100 agilent high sensitive dna chips

cDNA library preparation was performed using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, USA) according to the manufacturer’s protocol. In brief, the polyAcontaining mRNA molecules were purified from 4 μg of total RNA for each sample. After library preparation, cDNA library templates were generated. 2100 Agilent High Sensitive DNA chips (Agilent) were used for quality control. KAPA Library Quantification Kit (BioRad) was used for quantification and normalization before loading the samples onto an Illumina NextSeq 500 instrument for sequencing. Nextera 500 High Output v2 kit (150 cycles) was used for bulk cell RNA sequencing (RNA-Seq). PhiX Control v3 (Illumina) was used as a quality control for sequencing runs.

DNA sequencing libraries were prepared using the Hieff NGS C130P2 OnePot II DNA Library Prep Kit for MGI (Yeasen Biotechnology) according to the manufacturer's instructions. RNA was purified, and rRNA was removed from the purified RNA, A purified and amplified library was then constructed. The quality control was performed using 2100 Agilent High Sensitive DNA chips (Agilent),and libraries were sequenced by Illumina HiSeq 2000 on a single-end basis (50 bp).