Total RNA was extracted using the Hybrid-R RNA Extraction Kit (GeneAll, Seoul, Korea). cDNA was synthesized from total RNA (1 μg) using AmfiRivert cDNA Synthesis Platinum Master Mix (GenDEPOT). Real-time RT-PCR analysis was performed using SYBR mix (ELPIS Biotech, Daejeon, Korea) on a CFX384 real-time system, according to the manufacturer’s instructions (BioRad, Hercules, CA, USA). The mRNA level of individual genes was normalized by that of GAPDH. PCR primer sequences against individual genes are listed in Table 1 .
Sybr mix
Manufactured by Elpis Biotech
SYBR mix is a reagent used for real-time quantitative PCR (qPCR) analysis. It contains a fluorescent dye that binds to double-stranded DNA, allowing for the quantification of DNA amplification during the PCR process.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using sybr mix
1
Quantitative Gene Expression Analysis
2
RNA Extraction and RT-qPCR Analysis
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Total RNA was extracted using a Hybrid-R RNA extraction kit (GeneAll, Seoul, Republic of Korea). Synthesis of cDNA from total RNA (1 μg) was conducted using AmfiRivert cDNA Synthesis Platinum master mix (GenDEPOT, Austin, TX, USA). Real-time RT-PCR analysis was performed using SYBR mix (ELPIS Biotech, Daejeon, Republic of Korea) on the CFX384 real-time system as recommended by the manufacturer (BioRad, Hercules, CA, USA), and the mRNA level of individual genes was normalized by that of GAPDH. PCR primer sequences against individual genes are listed (Table 1 ).
3
Quantitative RT-PCR Analysis of A549 and Mouse Lung
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Total RNA of A549 cells and mouse lung tissues was extracted by Hybrid-R RNA extraction kit (GeneAll, Seoul, Korea). A total of 1 μg of total RNA was subject to cDNA synthesis, using PrimeScript RT-PCR kit (TAKARA Korea, Seoul, Korea). The real-time RT-PCR analysis was performed using SYBR mix (ELPIS Biotech. Daejeon, Korea) on CFX384 real-time system as recommended by manufacturer (BioRad, Hercules, CA, USA). The mRNA level of individual genes was normalized by that of GAPDH. The sequence of RT-PCR primers are listed in Table 1 .
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