Z0334

Manufactured by BD

Sourced in United Kingdom

The Z0334 is a laboratory equipment product manufactured by BD. It is designed to perform a core function within the laboratory setting. The product specifications and technical details are not available for an unbiased and factual description at this time.

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Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Ab11089, by Abcam (1 mentions) Alexa fluor 568 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Cm3050, by Leica camera (1 mentions) Mab5428, by Merck Group (1 mentions) Ab10558, by Agilent Technologies (1 mentions) Mabn50, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by BD (1 mentions) Sc 17320, by Abcam (1 mentions) Dm4000b, by Zeiss (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

3 protocols using z0334

Immunocytochemical Analysis of Glioma Stem Cells

Cited 1 time

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GIC-20 cells were plated at 50,000 cells per mL on
poly-D-lysine/laminin-coated coverslips in GIC media with 1 ng/mL EGF and basic
fibroblast growth factor (bFGF) and no leukemia inhibitory factor (LIF). RNA was
collected for qRT-PCR analysis or immunocytochemistry was performed as
previously described (Kouri et al., 2015 (link))
using the following antibodies: rabbit anti-GFAP (1:1000; DakoCytomation Z0334)
and mouse anti-MAP2 (1:500; BD Pharmingen 556320). Cells were imaged using a
Nikon A1R Spectral confocal microscope, and quantification was performed using
TissueGnostics LSC System, and data were analyzed with HistoQuest Software.

Calvert A.E., Chalastanis A., Wu Y., Hurley L.A., Kouri F.M., Bi Y., Kachman M., May J.L., Bartom E., Hua Y., Mishra R.K., Schiltz G.E., Dubrovskyi O., Mazar A.P., Peter M.E., Zheng H., James C.D., Burant C.F., Chandel N.S., Davuluri R.V., Horbinski C, & Stegh A.H. (2017). Cancer-associated IDH1 promotes growth and resistance to targeted therapies in the absence of mutation. Cell reports, 19(9), 1858-1873.

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Immunohistochemical Analysis of Enucleated Eyes

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Eyes were enucleated and placed in 4% paraformaldehyde (PFA) for less than 30 min, followed by gentle removal of anterior segments, including lens and vitreous. Whole eye cups were fixed in 4% PFA for 2 h at room temperature, rinsed with phosphate-buffered saline (PBS), cryoprotected with 30% sucrose overnight, embedded in optimal cutting temperature (OCT) medium, and then cryosectioned (18 μm) using a cryostat (CM3050 S, Leica). For immunohistochemistry, sections were washed with PBS, blocked with 10% normal donkey serum for 30 min, then incubated in primary antibody for 1–2 h at room temperature, followed by Alexa Fluor 568-conjugated secondary antibodies (Invitrogen). Primary antibodies include RPE65 (MAB5428, 1:250, Millipore), EGFP (Novousbio, NB100-1770, 1:500), IBA-1 (Wako, AB10558, 1:100), GFAP (Dako, Z0334, 1:200), CD45 (BD, 552566, 2.5 μg/mL), and CD3 (Abcam, ab11089, 1:100).

Yiu G., Chung S.H., Mollhoff I.N., Nguyen U.T., Thomasy S.M., Yoo J., Taraborelli D, & Noronha G. (2020). Suprachoroidal and Subretinal Injections of AAV Using Transscleral Microneedles for Retinal Gene Delivery in Nonhuman Primates. Molecular Therapy. Methods & Clinical Development, 16, 179-191.

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Immunofluorescence Staining of Tissue Sections

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Tissue was processed as described above. Sections or cells were incubated in sodium citrate (10 mM, microwave preheated) for antigen retrieval, blocked with PBS containing 0.3% (v/v) Triton X-100 and 10% (v/v) normal donkey serum (1 hr, RT), and incubated with primary antibody overnight, 4°C.
Primary antibody used: Caspase3 R and D Systems AF835-SP; CTGF Abcam ab6992; GFAP DakoCytomation Z0334; GFAP BD Biosciences 556330; GFP Abcam ab6673; MPZ Abcam ab39375; Neurofilament NF DakoCytomation M0762; Olig2 Abcam ab33427; Olig2 Millipore MABN50; Periaxin Gift from Prof. Peter Brophy, University of Edinburgh, UK; SMI71 Calbiochem NE1026; Sostdc1 Abcam 99340; Sox10 Gift from Dr. Michael Wegner, Universität Erlangen-Nürnberg, Erlangen, Germany; Sox2 Santa Cruz Biotechnology sc-17320; vWF Abcam ab6994; Iba1 Wako 019–19741, MBP R@D Systems MAB42282, tRFP Evrogen AB233.
Then, sections were incubated with appropriate AF488, AF555, or AT647- conjugated secondary antibodies (1:500, ThermoFisher Scientific). Nuclei were visualized with 4’, 6’-diamidino-2-phenylindole (DAPI; 0.1 mg/ml; Sigma). Images were acquired with fluorescence microscope (Leica DM4000B) and confocal (Zeiss LSM700) microscopes.

Ulanska-Poutanen J., Mieczkowski J., Zhao C., Konarzewska K., Kaza B., Pohl H.B., Bugajski L., Kaminska B., Franklin R.J, & Zawadzka M. (2018). Injury-induced perivascular niche supports alternative differentiation of adult rodent CNS progenitor cells. eLife, 7, e30325.

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