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Cfx manager 3.1 operational software

Manufactured by Bio-Rad
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CFX Manager 3.1 is an operational software developed by Bio-Rad for real-time PCR analysis. It provides instrument control and data analysis capabilities for Bio-Rad's CFX real-time PCR detection systems.

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Lab products found in correlation

Ssoadvanced universal sybr green supermix, by Bio-Rad (2 mentions) Iscript advanced cdna synthesis kit, by Bio-Rad (2 mentions) Cfx connect real time pcr system, by Bio-Rad (2 mentions)

Spelling variants of this product

CFX Manager software (1381 citations) CFX Manager 3.1 software (712 citations) CFX Manager (428 citations) CFX software (90 citations) CFX Manager Software 1.6 (15 citations) CFX 3.1 Manager software (9 citations) CFX 3.1 Manager (5 citations)

2 protocols using cfx manager 3.1 operational software

1

RT-qPCR Analysis of Gene Expression

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RT-qPCR (two-step) was performed using the iScript Advanced cDNA Synthesis Kit (BioRad) with 25 ng RNA/sample, followed by the SsoAdvanced Universal SYBR Green Supermix (BioRad) on a CFX Connect Real-Time PCR System (BioRad). Validated qPCR primers specific for human gene transcripts of CDKN1A (p21; qHsaCID0014498), along with ACTB (β-actin; qHsaCED0036269) and B2M (β2-microglobulin; qHsaCID0015347) reference standards, were utilized according to the manufacturer’s specifications (BioRad). Reference gene-normalized relative gene expression was calculated based on ΔΔCq values by the CFX Manager 3.1 operational software (BioRad).
Buzby J.S., Williams S.A., Schaffer L., Head S.R, & Nugent D.J. (2017). Allele-Specific Wild-Type TP53 Expression in the Unaffected Carrier Parent of Children with Li-Fraumeni Syndrome. Cancer genetics, 211, 9-17.
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2

Quantitative PCR Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
RT-qPCR (two-step) utilized iScript Advanced cDNA Synthesis Kits (Bio-Rad) with 25 ng RNA/sample, followed by the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) on a CFX Connect Real-Time PCR System (Bio-Rad). Validated qPCR primers specific for human gene transcripts of TP53 (p53, Bio-Rad; qHsaCID0013658), S100A4 (FSP-1, Bio-Rad; qHsaCID0013749), and the reference standard, HPRT1 (hypoxanthine phosphoribosyltransferase 1, Bio-Rad; qHsaCID0016375), were utilized according to the manufacturer’s specifications (Bio-Rad). Reference gene-normalized relative gene expression was calculated based on ΔΔCq values by the CFX Manager 3.1 operational software (Bio-Rad).
Buzby J.S., Williams S.A, & Nugent D.J. (2022). Unaffected Li-Fraumeni Syndrome Carrier Parent Demonstrates Allele-Specific mRNA Stabilization of Wild-Type TP53 Compared to Affected Offspring. Genes, 13(12), 2302.
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