Statistical analysis of the data was performed using GraphPad Prism 8.4.3 for Windows. For each experiment, the quantitative data was tested for normality and equality of variances. On the basis of the number of groups present in each experiment and the outcome of the normality and variance tests, the appropriate statistical tests (described in the figure legends) were selected to analyze the data. Differences between groups were considered significant at p < 0.05. All data are expressed as mean ± SEM.
Prism 8.4.3 for windows
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GraphPad Prism 8.4.3 for Windows is a data analysis and graphing software. It provides tools for curve fitting, statistical analysis, and visualization of scientific data.
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Lab products found in correlation
16 protocols using prism 8.4.3 for windows
1
Statistical Analysis of Experimental Data
2
Standard Statistical Analyses for Research
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Standard statistical analyses (average, standard deviation, and ANOVA) were performed using GraphPad Prism 8.4.3 for Windows.
3
Analyzing Cell Proliferation Dynamics
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Microsoft Excel office 365 and GraphPad Prism 8.4.3 for Windows were used for graphing and analysis of the data. Ordinary one-way ANOVA with Tukey multiple comparisons was applied to calculate P-values.
4
Retinal Function and Cell Counts
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Data are represented as the arithmetic mean ± SEM of all samples in the corresponding experimental groups run in at least duplicate. Differences between comparative groups were interpreted as being statistically significant for P < 0.05. We compared experimental groups for ERG, MPO, and CFUs per eye using the Mann-Whitney U test. All statistical analyses performed for experiments described in this paper were executed using Prism 8.4.3 for Windows (GraphPad, Boston, MA, USA).
5
Statistical Analysis of Research Findings
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Statistical analysis was conducted in Prism 8.4.3 for windows (GraphPad Software, San Diego, CA, USA, www.graphpad.com (accessed on 7 December 2022)) via Mann–Whitney U-Test with p ≤ 0.05 deemed significant and 0.05 < p < 0.1 deemed trending. All values are expressed as mean ± SEM.
6
Statistical Analysis of Experimental Data
7
Statistical Analysis of Ocular Measurements
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Data are the arithmetic means ± SEM of all samples in the same experimental group in replicate experiments. Comparative differences between groups were taken to be statistically significant when P < 0.05. The Mann–Whitney U test was used to compare experimental groups for ERG, MPO, and bacterial counts per eye. All statistical analyses were performed using Prism 8.4.3 for Windows (GraphPad, San Diego, CA, USA).
8
Statistical Analysis of Experimental Data
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Statistical data processing was performed using GraphPad Prism 8.4.3 for Windows (GraphPad Software, La Jolla, CA, USA). The distribution of the results was analyzed using Shapiro–Wilk test. Student’s t-test was used in case of normal distribution, and Mann–Whitney U-test was used to compare data that were not normally distributed. Results are presented as median (min–max). Statistical significance was established at p < 0.05.
9
Statistical Analysis of Experimental Data
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Data were processed statistically in GraphPad Prism 8.4.3 for Windows (GraphPad Software, La Jolla, CA, USA). The distribution of the results was analyzed using the Shapiro–Wilk test. A Student’s t-test was used for normal distribution, and the Mann–Whitney U test was applied to compare data that were not normally distributed. The results were presented as median values (minimum–maximum). Statistical significance was established at p < 0.05.
10
Propolis Extract Inhibits SU-DHL-2 Cells
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SU-DHL-2 cell (ATCC CRL-2956, purchased from Cellcook, Guangzhou, China) was cultured with the medium composed of 89% the modified RPMI-1640 basal medium (purchased from Wuhan Prosper Life Technology Co., Ltd. Wuhan, China), 10% fetal bovine serum (purchased from Cellmax Bio Co., Ltd. Lanzhou, China), and 1% penicillin and streptomycin mixture (purchased from HyClone Biochemical Products Co., Ltd. Shanghai, China) in a 5% CO2 humidified incubator at 37 °C (C150, Binder, Tuttlingen, German).
Propolis extract was dissolved in DMSO at a concentration of 2 mg/mL. Propolis solution was diluted with a complete medium for 100, 50, 25, 12.5, 6.25, 5, and 2 µg/mL. A complete medium added DMSO (0.25%, V/V; equal to DMSO in 100 ug/mL propolis group) was designed as a negative control. They were used to culture SU-DHL-2 cells at a beginning concentration of 5 × 105 cells/mL. Then, the cell suspensions were collected after 24 h to determine cell viability by CCK8 kit (Purchased from DOJINDO, Kumamoto, Japan) at 450 nm using a microplate reader (1510, Thermo Fisher Waltham, MA, USA). IC50 of propolis extract on SU-DHL-2 cell for 24 h was calculated using Graphpad Prism 8.4.3 for Windows (GraphPad Software, Inc., La Jolla, CA, USA).
Propolis extract was dissolved in DMSO at a concentration of 2 mg/mL. Propolis solution was diluted with a complete medium for 100, 50, 25, 12.5, 6.25, 5, and 2 µg/mL. A complete medium added DMSO (0.25%, V/V; equal to DMSO in 100 ug/mL propolis group) was designed as a negative control. They were used to culture SU-DHL-2 cells at a beginning concentration of 5 × 105 cells/mL. Then, the cell suspensions were collected after 24 h to determine cell viability by CCK8 kit (Purchased from DOJINDO, Kumamoto, Japan) at 450 nm using a microplate reader (1510, Thermo Fisher Waltham, MA, USA). IC50 of propolis extract on SU-DHL-2 cell for 24 h was calculated using Graphpad Prism 8.4.3 for Windows (GraphPad Software, Inc., La Jolla, CA, USA).
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