Lipofectmin 2000

Manufactured by Thermo Fisher Scientific

Sourced in United States, China

Lipofectamin 2000 is a lipid-based transfection reagent. It is used for the delivery of nucleic acids, such as DNA and RNA, into mammalian cells.

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2 protocols using lipofectmin 2000

Visualizing FOXM1 and FOXA2 Localization

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293T cells (2 × 10⁵ cells in a 60 mm plate) were transiently transfected by Lipofectmin 2000 (Invitrogen, USA) according to the manufacturer’s instructions with pCMV-FOXM1-GFP (5 μg) or pCMV-FOXA2-GFP (5 μg) and 24 hr later were transfected with M-FOXM1 Apt-F (1 μM) or M-Control Apt-F (1 μM) separately. The localization of aptamers and FOXM1 or FOXA2 were imaged with a confocal imaging system (FluoView FV1000, Olympus, Japan), with the ROX fluorescent dye and GFP fluorescent proteins activated by laser at 559 nm and 488 nm respectively.

Xiang Q., Tan G., Jiang X., Wu K., Tan W, & Tan Y. (2017). Suppression of FOXM1 Transcriptional Activities via a Single-Stranded DNA Aptamer Generated by SELEX. Scientific Reports, 7, 45377.

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Comprehensive Protein Analysis Protocol

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Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime Biotech, China); Lipofectmin 2000 (Invitrogen, USA); Entranster™-in vivo transfection reagent (Engreen Biosystem, China); pifithrin-α (Sigma, USA); fluvastatin (Novartis Pharma, Switzerland); 3-methyadenine (Sigma, USA); Bafilomycin A1 (Santa Cruz, CA, USA), chloroquine (MedChemExpress, USA), denosumab (Prolia, Amgen Inc., USA); mouse-anti-AMPKα1/2 (sc-74461, Santa Cruz, USA); rabbit-anti-pAMPKα1/2 (Thr 172) (sc-33524, Santa Cruz, USA); rabbit-anti-ACCα (sc-30212, Santa Cruz); rabbit-anti-ACCα (sc-271965, Santa Cruz); mouse-anti-PTEN (sc-7974, Santa Cruz); rabbit-anti-AKT (sc-8312, Santa Cruz); rabbit-anti-pAKT (Ser 473) (sc-33437, Santa Cruz); rabbit-anti-Histone H3 (sc-10809, Santa Cruz); mouse-anti-p53 (sc-126, Santa Cruz); rabbit-anti-LC3 (sc-28266, Santa Cruz); rabbit-anti-mTOR (sc-8319, Santa Cruz); rabbit-anti-mTOR (Ser 2448) (sc-101738, Santa Cruz); mouse-anti-β-Actin (sc-47778, Santa Cruz).

Yang Z., Su Z., DeWitt J.P., Xie L., Chen Y., Li X., Han L., Li D., Xia J., Zhang Y., Yang Y., Jin C., Zhang J., Li S., Li K., Zhang Z., Qu X., He Z., Chen Y., Shen Y., Ren M, & Yuan Z. (2017). Fluvastatin Prevents Lung Adenocarcinoma Bone Metastasis by Triggering Autophagy. EBioMedicine, 19, 49-59.

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