For live-cell imaging cells were mounted on ME agarose pads, containing 2% agarose (Tran et al., 2004 (link)). Imaging was performed at 25°C. Images were acquired on an inverted Spinning Disk Confocal (Roper/Nikon), equipped with Plan Apochromat 100 × /1.4 NA objective lens (Nikon), a PIFOC (perfect image focus) objective stepper, and a charge-coupled device camera (EMCCD 512×512 QuantEM; Photometrics). Stacks of 7 planes spaced 1 μm were acquired for each channel with 100 ms exposure for 561 nm wavelength and 100 ms exposure for 491 nm wavelength, binning one, and an electronic gain of 3 for both wavelengths. For time-lapse movies, generally, an image was taken every minute, or every ten minutes, as it was the case for the nda2noD mutant, for a duration of 240 min.
Plan apochromat 100 1.4 na objective lens
Manufactured by Nikon
The Plan Apochromat 100× /1.4 NA objective lens is a high-performance microscope objective designed for use in advanced laboratory applications. It features a numerical aperture of 1.4 and a 100× magnification, providing users with excellent optical resolution and image quality. The lens is designed to minimize chromatic and spherical aberrations, ensuring accurate and faithful reproduction of samples.
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Lab products found in correlation
2 protocols using plan apochromat 100 1.4 na objective lens
1
Live-Cell Imaging of Cellular Dynamics
2
Live-cell Imaging of Fission Yeast
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For live-cell imaging, fission yeast cells were mounted on YE5S agarose pads, containing 2% agarose (Tran et al., 2004 (link)). Temperature-sensitive cut7-24 and sad1-1 mutants were incubated at the microscope, which is equipped with a cage incubator (Life Imaging Services), at 37°C for 1 hr before imaging was started. All other strains were imaged at 25°C.
Images were acquired on an inverted Eclipse Ti-E microscope (Nikon) with a spinning disk CSU-22 (Yokogawa), equipped with a Plan Apochromat 100×/1.4 NA objective lens (Nikon), a PIFOC (perfect image focus) objective stepper, a Mad City Lab piezo stage, and an electron-multiplying charge-couple device camera (EMCCD 512 × 512 Evolve, Photometrics).
Stacks of seven planes spaced by 1 µm were acquired for each channel with 100 ms exposure time, binning one and an electronic gain of 300 for both wavelengths. For each time-lapse movie, an image was taken every minute for 90–120 min.
Images were acquired on an inverted Eclipse Ti-E microscope (Nikon) with a spinning disk CSU-22 (Yokogawa), equipped with a Plan Apochromat 100×/1.4 NA objective lens (Nikon), a PIFOC (perfect image focus) objective stepper, a Mad City Lab piezo stage, and an electron-multiplying charge-couple device camera (EMCCD 512 × 512 Evolve, Photometrics).
Stacks of seven planes spaced by 1 µm were acquired for each channel with 100 ms exposure time, binning one and an electronic gain of 300 for both wavelengths. For each time-lapse movie, an image was taken every minute for 90–120 min.
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