Anti rab27b

After quantitative analysis, the total protein of the sample was extracted and denatured in this study. SDS–PAGE gel was used for electrophoresis, electrophoresis apparatus (Bio‐RAD) was adjusted to 120 V for electrophoresis, PVDF membrane (Millipore) was used for membrane transfer, and skim milk (Sigma) was used for blocking. Primary antibodies (Abcam) were then added and incubated overnight. The antibodies used were as follows: anti‐Rab11 (Abcam, 1:1000), anti‐Rab27a (Abcam, 1:200), anti‐Rab27b (Abcam, l:500), anti‐TSAP6 antibody (Abcam, 1:1000), anti‐CD63 antibody (Abcam, 1:1000), anti‐CD9 antibody (Abcam, 1:1000), anti‐Alix antibody (Abcam, 1:1000), and anti‐β‐Actin antibody (Abcam, 1:10,000). The membrane was then incubated with the primary antibody diluted in advance. Subsequently, the membranes were incubated with IgG (Abcam, 1:300) for 1 h. Finally, the protein bands were visualized using a Pierce ECL Western blotting kit. Protein expression was quantified using ImageJ to analyze band gray values. β‐Actin was used as a control.

Protein levels were analyzed using IB and ELISA following conventional protocols. For IB, the primary antibodies used were anti-YAP (Abcam, Cambridge, MA, USA, #ab52771), anti-GAPDH (CST, Boston, MA, USA, #5174 and #51332), anti-p-127-YAP (Abcam, #ab76252), anti-p-381-YAP (CST, #13619), anti-O-Glc-YAP (developed by Biolynx, Hangzhou, China), anti-HRS (Abcam, #ab72053), anti-PLD2 (CST, #13904), anti-RAB2B (Novus, Littleton, CO, USA, #NBP1-31631), anti-RAB27A (Abcam, #ab55667), anti-RAB27B (Abcam, #ab103418), anti-VAMP (Abcam, #ab36195), anti-ATG7 (Abcam, #ab52472), anti-SOX10 (Abcam, #ab227680), anti-Mycn (Abcam, #ab227822), anti-ALYREF (Abcam, #ab202894), anti-NSUN2 (Abcam, #ab259941), anti-DNMT2 (Abcam, #ab220175), anti-NSUN1 (Abcam, #ab270175), anti-NSUN3 (Abcam, #ab272616), anti-NSUN4 (Abcam, #ab235430), anti-NSUN5 (Abcam, #ab121633), anti-NSUN6 (Novus, #NBP1-92202), anti-NSUN7 (Biorbyt, Cambridge, UK, #orb258175), anti-YBX1 (Abcam, #ab255606), anti-EXOSC10 (Abcam, #ab94981), anti-SPI1 (Abcam, #ab227835), anti-CD63 (Abcam, #ab271286), anti-TSG101 (Abcam, #12501), anti-ALIX (Abcam, #ab275377), anti-CD9 (Abcam, #ab92726), and anti-calnexin (Abcam, #ab133615). For ELISA, YAP and NSUN2 levels were measured using kits from Yingxin Biotech Ltd. (Shanghai, China) as per manufacturer’s instructions.