Cocktails of the monoclonal antibodies including Cab3, anti-CII antibody cocktail (positive control), and isotype antibody cocktail (negative control) were prepared by mixing equal amounts of each sterile filtered antibody solution to achieve a final concentration of 2 or 4 mg. Mice were injected intravenously (i.v.) with the antibody cocktail (Cab3) composed of M2139, L10D9, and 15A11. For the induction of lpsCAIA (Cab-induced and LPS-enhanced arthritis), the mice received (50 μg/mouse) lipopolysaccharide from Escherichia coli O55: B5 (Sigma-Aldrich) intraperitoneally (i.p.) on day 5. For the induction of mCAIA (Cab-induced mannan-enhanced arthritis), the mice received (5 mg/mouse) mannan (Sigma-Aldrich) from Saccharomyces cerevisiae i.p. on days 5 and 60. Signs of arthritis in the paws were followed up macroscopically with blind scoring of each red and swollen joint following a previously described protocol [35 ]. Briefly, macroscopic (clinical) arthritis was defined based on the two following criteria, swelling and redness, and was scored as follows: 1 point was for each inflamed toe or knuckle, whereas 1–5 points were given to an inflamed wrist or ankle according to the severity of disease, resulting in a score of 0 to 15 for each paw and 0 to 60 points for each mouse. Importantly, paws which were still swollen but without erythema were not scored or defined as arthritis.
Lipopolysaccharide from escherichia coli o55 b5
Manufactured by Merck Group
Sourced in United States
Lipopolysaccharide from Escherichia coli O55:B5 is a complex molecule derived from the outer membrane of the Gram-negative bacterium Escherichia coli. It consists of a lipid component and a polysaccharide component. This product is commonly used for research and scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
Mannan, by Merck Group (1 mentions)
Phosphoric acid, by Thermo Fisher Scientific (1 mentions)
Potassium dihydrogen orthophosphate, by Thermo Fisher Scientific (1 mentions)
Tego care 450, by Evonik (1 mentions)
Poloxamer 188 p188, by BASF (1 mentions)
Tween 20, by Croda (1 mentions)
Oleic acid, by Croda (1 mentions)
Mouse anti actin antibody, by Merck Group (1 mentions)
α asarone, by Merck Group (1 mentions)
Mouse anti tnfα antibody, by Santa Cruz Biotechnology (1 mentions)
Goat anti mouse igg horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Tri reagent, by Merck Group (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Dnase 1, by Merck Group (1 mentions)
Cellmask green actin tracking stain, by Thermo Fisher Scientific (1 mentions)
Taqman fast advanced master mix, by Thermo Fisher Scientific (1 mentions)
Negative sirna control, by Thermo Fisher Scientific (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
5 protocols using lipopolysaccharide from escherichia coli o55 b5
1
Monoclonal Antibody Cocktail Arthritis Induction
2
Formulation and Characterization of CBD-Loaded Lipid Nanocarriers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cetyl palmitate (CP) was obtained from SABO S.p.A. (Levate (BG), Italy). Labrafac lipophile WL1340 (medium chain triglycerides, MCT) and Transcutol® P (diethylene glycol monoethyl ether) were gifts from Gattafossé (Cedex, France). Tego® Care 450 (TG450, polyglyceryl-3 methylglucose distearate) was obtained from Evonik Industries AG (Essen, Germany). Poloxamer 188 (P188) was provided by BASF (Ludwigshafen, Germany). Tween 20 and oleic acid (OA) were obtained from Croda (Singapore). CBD extract (87% CBD purity) was purchased from Greenleaf (Bangkok, Thailand). Lipopolysaccharide from Escherichia coli O55:B5 was obtained from Sigma-Aldrich (Missouri, USA). Sodium chloride was acquired from Carlo Erba Reagenti (Cornaredo MI, Italy). Phosphoric acid and potassium dihydrogen orthophosphate were obtained from Fisher Scientific (Loughborough, UK). Methanol was of HPLC grade.
3
Neuroinflammation Modulation by α-Asarone
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
α-Asarone (trans-1-Propenyl-2,4,5-trimethoxybenzene, Fig. 1 ) and lipopolysaccharide (from Escherichia coli O55:B5) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse anti-TNF-α antibody, goat anti-rabbit and goat anti-mouse IgG horseradish peroxidase (HRP) conjugated secondary antibody were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-amyloid precursor protein (APP) antibody was purchased from Millipore (Billerica, MA, USA). Rabbit anti-IL-1β and rabbit anti-β-site APP-converting enzyme 1 (BACE1) antibodies were purchased from Abcam (Cambridge, UK). Rabbit anti-ionized calcium binding adaptor molecule 1 (Iba1) antibodies (#016-20001, #019-19741) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Mouse anti-actin antibody was purchased from Chemicon International (Temecula, CA, USA). The other chemicals and reagents used were of high quality and obtained from various commercial sources.
4
Microglial Phagocytosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Reagents for reverse transcription (High-Capacity cDNA Reverse Transcription Kit with RNase Inhibitor) and quantitative PCR (Taqman Assays, TaqMan OpenArray Mouse Phagocytosis Panel, and TaqMan Fast Advanced Master Mix), red fluorescent microspheres (FMS), 2.0 μm, Hoechst 33342, CellMask Orange Actin Tracking Stain, CellMask Green Actin Tracking Stain, RPMI were obtained from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Opti-MEM was from Gibco, negative siRNA control was from Ambion, Lipofectamine was from Thermo Fisher Scientific, Inc., Aβ1–42 and Aβ1–42 HiLyte 488 were from AnaSpec, Inc. (Fremont, CA, USA). JQ1, GSK12101517, IBET-762, OTX-015, PFI-1, Cytochalasin D were obtained from Sigma-Aldrich (St. Louis, MO, USA). Heat-inactivated foetal bovine serum (FBS), Accutase solution, penicillin, streptomycin, L-glutamine, 3-(4,5-dimethyl-2-tiazolilo)-2,5-diphenyl-2H-tetrazolium bromide (MTT), propidium iodide, TRI-reagent, DNase I, dithiothreitol (DTT), anhydrous dimethyl sulfoxide (DMSO), lipopolysaccharide from Escherichia coli O55:B5 (toxicity 3,000,000 U/mg), and all other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA).
5
Osteoclastogenesis Regulation by Epothilone B
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Epothilone B (Epo B) was purchased from MedChemExpress (Princeton, NJ). Recombinant Mouse M-CSF and Recombinant Mouse RANKL were obtained from R&D Systems (Minneapolis, MN). TRAP stain kit and Lipopolysaccharide from Escherichia coli O55:B5 were purchased from Sigma-Aldrich (St. Louis, USA). Antibodies against c-FOS, NFATc1, IL-6 and gp130 were from Cell Signaling Technology Inc (Danvers, USA). Antibodies against phospho-NFκB p65, NF-κB p65, phospho-IκBα, IκBα, STAT3, phospho-STAT3 (Tyr705) and β-actin were from Bioworld Technology (St. Louis Park, MN, USA). Antibody against Akt, phospho-Akt (Ser473), PI3K, phospho-PI3K (Ser1070), MMP9, CD9 and CTSK were purchased from bioss (Beijing, China). Nitric oxide detection kit (Beyotime Biotechnology, Shanghai, China). CCK-8 kit was purchased from Dojindo Molecular Technologies (Tokyo, Japan). The Focal Adhesion and Actin Cytoskeleton Staining Kit was obtained from Millipore (Darmstadt, Germany). Bovine cortical bone slices for pit formation assay were obtained from Boineslices.com (Jelling, Denmark). Dulbecco’s modified eagle medium (DMEM), alpha minimal essential medium (α-MEM), and fetal bovine serum (FBS) were purchased from Gibco (Life Technologies, USA). RAW264.7 cells (mouse macrophage cells) were obtained from the American Type Culture Collection (Rockville, MD, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!