Culture supernatants from primary T cells were collected at 48 h and analyzed for IL-2, IL-4, IL-10, IFN-γ, and IL-1α using the Milliplex MAP mouse cytokine/chemokine immunoassay kit (Millipore) on a Luminex (Bio-Rad) instrument. For hybridomas, supernatant was assessed at 24 h for IL-2 only. For intracellular cytokine staining, cells were cultured with Cell Stimulation Cocktail and 10 μg/ml monensin (eBioscience), for 4 h at 37 °C, followed by fixation, permeabilization, and intracellular staining for IL-17A and IFN–γ.
Milliplex map mouse cytokine chemokine immunoassay kit
Manufactured by Merck Group
The Milliplex MAP mouse cytokine/chemokine immunoassay kit is a multiplex assay designed to simultaneously detect and quantify multiple mouse cytokines and chemokines in a single sample. The kit utilizes Luminex technology to enable the measurement of these analytes.
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Lab products found in correlation
2 protocols using milliplex map mouse cytokine chemokine immunoassay kit
1
Multiplex Cytokine Analysis in T Cells
2
Cytokine Quantification of Hybridoma Supernatants
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