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Diethyl phosphite

Manufactured by Merck Group
Sourced in United States, Germany

Diethyl phosphite is a colorless, flammable liquid that is commonly used as a laboratory reagent. It is a phosphorus-containing compound with a chemical formula of (C2H5O)2PH. Diethyl phosphite is known for its reducing properties and is often employed in organic synthesis reactions.

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5 protocols using diethyl phosphite

1

Reagents and Materials for Peptide Synthesis

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Example 1

Reagents for peptide synthesis were purchased from Chem-Impex (Wood Dale, Ill.), NovaBiochem (La Jolla, Calif.), or Anaspec (San Jose, Calif.). Rink amide resin LS (100-200 mesh, 0.2 mmol/g) was purchased from Advanced ChemTech. Cell culture media, fetal bovine serum, penicillin-streptomycin, 0.25% trypsin-EDTA, and DPBS were purchased from Invitrogen (Carlsbad, Calif.). Methyl 3,5-dimethylbenzoiate, N-bromosuccinimide, diethyl phosphite, 2,2′-dipyridyl disulfide, and other organic reagents/solvents were purchased from Sigma-Aldrich (St. Louis, Mo.). Anti-GST-Tb and streptavidin-d2 were purchased from Cisbio (Bedford, Mass.). The NF-κB reporter (Luc)-HEK293 cell line and One-Step™ luciferase assay system were purchased from BPS Bioscience (San Diego, Calif.).

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2

Reagents and Materials for Peptide Synthesis

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Example 1

Reagents for peptide synthesis were purchased from Chem-Impex (Wood Dale, Ill.), NovaBiochem (La Jolla, Calif.), or Anaspec (San Jose, Calif.). Rink amide resin LS (100-200 mesh, 0.2 mmol/g) was purchased from Advanced ChemTech. Cell culture media, fetal bovine serum, penicillin-streptomycin, 0.25% trypsin-EDTA, and DPBS were purchased from Invitrogen (Carlsbad, Calif.). Methyl 3,5-dimethylbenzoiate, N-bromosuccinimide, diethyl phosphite, 2,2′-dipyridyl disulfide, and other organic reagents/solvents were purchased from Sigma-Aldrich (St. Louis, Mo.). Anti-GST-Tb and streptavidin-d2 were purchased from Cisbio (Bedford, Mass.). The NF-κB reporter (Luc)-HEK293 cell line and One-Step™ luciferase assay system were purchased from BPS Bioscience (San Diego, Calif.).

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3

Peptide Synthesis Reagents and Cell Culture Protocols

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Example 1

Reagents for peptide synthesis were purchased from Chem-Impex (Wood Dale, IL), NovaBiochem (La Jolla, CA), or Anaspec (San Jose, CA). Rink amide resin LS (100-200 mesh, 0.2 mmol/g) was purchased from Advanced ChemTech. Cell culture media, fetal bovine serum, penicillin-streptomycin, 0.25% trypsin-EDTA, and DPBS were purchased from Invitrogen (Carlsbad, CA). Methyl 3,5-dimethylbenzoiate, N-bromosuccinimide, diethyl phosphite, 2,2′-dipyridyl disulfide, and other organic reagents/solvents were purchased from Sigma-Aldrich (St. Louis, MO). Anti-GST-Tb and streptavidin-d2 were purchased from Cisbio (Bedford, MA). The NF-κB reporter (Luc)-HEK293 cell line and One-Step™ luciferase assay system were purchased from BPS Bioscience (San Diego, CA).

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4

Magnetic Iron Oxide Encapsulation

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Polyvinyl alcohol (PVA), carbonyl iron, glutaraldehyde (GA), hydrochloric acid, diethyl phosphite, p-toluenesulfonic acid, dioxane, tetrahydrofuran, palladium(II) chloride, copper(II) chloride, chloroform, and potassium carbonate were products of Sigma Aldrich (Saint Louis, MO, USA). dioxane and tetrahydrofuran were dried over sodium metal followed by distillation. The encapsulation of carbonyl iron was carried out according to the procedure described in [36 (link)].
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5

Synthesis of Phosphate-containing Epoxy Resins

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9,10-dihydro-9-oxa-10-phosphaphenanthrene-10-oxide (DOPO) and 3,5-bis(trifluoromethyl)phenyl isothiocyanate (purity 98%) were acquired from TCI. Poly(propylene glycol) diglycidyl ether PPO GE (Mn = 640 g·mol−1), poly(methylhydrosiloxane) (PMHS, Mn = 2080 g·mol−1)[46 (link)], 4,4′-methylenebis(N,N-diglycidylaniline) (MBDA), tetrabutylammonium bromide (TBAB) (purity 99%), 2,2′-biphenol, phosphorus trichloride (PCl3), diphenyl phosphite (DPP), diethyl phosphite (DEP), anhydrous tetrahydrofuran, lithium bis(trimethylsilyl)amide solution (LiHMDS 1M in THF), boron trifluoride diethyl etherate (BF3.Et2O) and cyclohexylamine, were purchased from Sigma-Aldrich (Darmstadt, Germany). Jeffamine® EDR-148 (2,2′-(ethylenedioxy)bis(ethylamine)) was kindly supplied by Huntsman. CO2 was acquired from Air Liquide. Sodium hydroxide (NaOH), ethyl acetate (AcOEt), dioxane, tetrahydrofuran (THF) and dichloromethane (DCM) were purchased from VWR International S.A.S (Fontenay-sous-Bois, France). The NMR solvents used are CDCl3 and DMSO-d6 from Eurisotop.
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