Serum IgE concentrations were measured using Mouse IgE ELISA Ready-SET-Go kit (eBioscience). Serum IgG1 and IgG2b were measured using Mouse Ig Isotyping ELISA Ready-Set-Go kit (eBioscience). Experiments were performed following the manufacturer’s instructions.
Mouse ige elisa ready set go kit
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Mouse IgE ELISA Ready-SET-Go kit is a laboratory product used for the quantitative measurement of mouse immunoglobulin E (IgE) levels in biological samples. It is an enzyme-linked immunosorbent assay (ELISA) kit that provides all the necessary reagents and materials to perform the IgE detection and quantification.
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6 protocols using mouse ige elisa ready set go kit
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Measurement of Serum Ig Levels
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Measuring Mouse and Human Immune Responses
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Total mouse serum IgE was measured by ELISA using Mouse IgE ELISA Ready-SET-Go kit (eBioscience). OVA-specific IgE was measured by a modified assay such that the plates were coated overnight with 200 μg/ml OVA rather than anti-IgE capture antibody and the rest of the test was similar to the total serum IgE ELISA assay. Mouse IL-4, IL-5, IL-13, IL-17 IFN-γ and Eotaxin cytokines were analyzed in BAL fluids collected from mice using eBioscience ELISA kits, according to the manufacturer's protocol. Human IL-13 and IL-17 were analyzed in culture supernatants of human peripheral blood cells activated with anti-CD3 + anti-CD28 mAbs in the absence or added presence of TGF β or TGF β + IL-4 using eBioscience ELISA kits.
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Measurement of Serum Ig Levels
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Serum Immunoglobulin and Cytokine Profiling
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Peripheral blood samples were collected for serum preparation. The level of IgE in serum was determined using the mouse IgE ELISA Ready-SET-Go kit (eBioscience) and the concentration of IgE was calculated using GraphPad Prism 6 (GraphPad Software, Inc). The levels of IL-2 (171-G5003M), IL-4 (171-G5005M), IL-6 (171-G5007M), IL-17 (171-G5013M), IFN-γ (171-G5017M), and TNF (171-G5023M) was determined by Bio-Plex (Biorad) according to the manufacturer's conditions.
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Measuring Mouse and Human Immune Responses
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Evaluating Serum IgE and Skin Pathology in AD Mouse Model
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Level of serum IgE in an AD-like mouse model. Blood samples were collected directly from the inferior vena cava using a capillary tube at the end of the experiment. Serum was obtained by centrifugation at 3,000 x g for 10 min at 4˚C and stored at -70˚C until use. Serum IgE levels were measured using a mouse IgE ELISA Ready-Set-Go kit (eBiosciences, San Diego, CA, USA) according to the manufacturer's instructions.
Histopathological alterations in an AD-like mouse model. Dermis tissue was fixed by inflating the tissue with 10% formalin. The tissues were then embedded in paraffin, cut into sections (5 µm) and stained with hematoxylin and eosin (H&E). All tissue samples were examined and images were captured and scored in a blinded manner under a light microscope (BX51; Olympus, Tokyo, Japan). Images were captured on an Olympus DP controller and manager under a microscope at (BX51; Olympus) x100 magnification. For examination of the distribution of mast cells beneath the dermis and hypodermis, the prepared tissues were stained with toluidine blue and images were captured on an Olympus DP controller and manager under a microscope (BX51; Olympus) at x400 magnification. The number of mast cells in a 1 mm 2 area was counted under a microscope at x400 magnification.
Histopathological alterations in an AD-like mouse model. Dermis tissue was fixed by inflating the tissue with 10% formalin. The tissues were then embedded in paraffin, cut into sections (5 µm) and stained with hematoxylin and eosin (H&E). All tissue samples were examined and images were captured and scored in a blinded manner under a light microscope (BX51; Olympus, Tokyo, Japan). Images were captured on an Olympus DP controller and manager under a microscope at (BX51; Olympus) x100 magnification. For examination of the distribution of mast cells beneath the dermis and hypodermis, the prepared tissues were stained with toluidine blue and images were captured on an Olympus DP controller and manager under a microscope (BX51; Olympus) at x400 magnification. The number of mast cells in a 1 mm 2 area was counted under a microscope at x400 magnification.
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